FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l04n02 tsrexp_lib#34.3420000000cdc4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l04n02 tsrexp_lib#34.3420000000cdc4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7233938
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGCC8700.027.57700742-43
GGCGCCC2950.027.37611644-45
GGGGCGC8900.023.48754742-43
GGGGGCG10050.020.80727240-41
TCGGGGG14800.019.91079738-39
GGGCCCG2400.017.81460444-45
CGGGTAG6800.016.7690714-15
CTCGGGG12500.016.72951136-37
GGGGGGC12600.015.84190140-41
GGGTAGA18250.015.48432228-29
GTAGGGG11400.015.41998418-19
CGGGGGG16100.013.87492538-39
AGGGTAG19250.013.56969428-29
GAGCGGC17650.013.4570289
GGGGCCC4600.012.90913444-45
GGGCGGG8000.012.1744112-13
GCGCCCG5400.011.87640344-45
TAGGGGA15550.011.76298118-19
CGCGAAT9700.011.257821
CGAAAAT50250.011.1492121
Produced by FastQC (version 0.11.2)