FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l04n02 tsrexp_lib#31.3420000000cd5b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l04n02 tsrexp_lib#31.3420000000cd5b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10000442
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGCC15200.024.37856342-43
GGGGGCG14400.021.78029640-41
TCGGGGG22500.021.1212438-39
GGCGCCC4450.020.81780644-45
GGGGCGC15400.019.12617342-43
GGGGGGC17050.017.69830540-41
GGGGCCC5600.016.96689444-45
GGGCCCG3650.016.92040844-45
GGGTAGA27100.016.91273728-29
CGGGTAG10000.016.15325214-15
AGGGTAG27350.015.88984428-29
CTCGGGG21900.014.97248336-37
CGGGGGG23200.014.44119338-39
GGGCGGG12250.013.57351612-13
CGGGCAT12100.013.3415341
GAGGGGC13200.013.3150399
CGGGGGT9000.013.20077538-39
GTAGGGG18650.012.86481118-19
GCGGGCT11100.012.4047281
CGAAAAT69050.012.1021131
Produced by FastQC (version 0.11.2)