FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l04n02 tsrexp_lib#3.3420000000ca27.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l04n02 tsrexp_lib#3.3420000000ca27.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8566489
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG179580.20963080674007753Illumina Single End PCR Primer 1 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGCGC19700.032.9172942-43
GGCGCCC7100.031.44811444-45
TCGGGGG34650.031.4071438-39
GGGCGCC26800.029.24871442-43
GGGCCCG3950.028.26349644-45
GGGGGCG24800.027.40076640-41
AGGGTAG32350.026.57397328-29
AAGGGGC15950.026.5062079
CTCGGGG29600.025.92741236-37
GTAGGGG19200.024.74590918-19
GATCGGG31150.024.5413271
GGGGGGC28900.023.92453640-41
CGGGTAG15950.023.532814-15
GGGTAGA38700.023.44091628-29
CGGGGGG31100.022.7678538-39
CGGGGGT13750.022.29221538-39
GAGGGGC14150.021.8210289
GGGTAGG19050.020.82583816-17
GAGCGGC29300.020.427789
GGGCGGG17550.020.16738112-13
Produced by FastQC (version 0.11.2)