FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l04n02 tsrexp_lib#26.3420000000ccef.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l04n02 tsrexp_lib#26.3420000000ccef.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9130119
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG110550.12108275916228473Illumina Single End PCR Primer 1 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCCC4650.033.20433844-45
GGGCGCC14700.028.60262142-43
GGGGCGC14700.025.37068742-43
TCGGGGG23850.025.30665638-39
GGGGGCG15750.023.68581440-41
GGGGGGC17800.019.7565440-41
CTCGGGG21200.019.05419536-37
AGGGTAG26700.018.4109228-29
GGGGCCC5250.018.09818844-45
GTAGGGG16750.018.01158518-19
GCGCCCG6650.017.86005444-45
CGGGGGT10500.017.65203538-39
GGGTAGA31550.016.70975128-29
CGGGGGG22200.016.59083238-39
GGGGGTC11900.016.37329540-41
GGGCGGG13800.015.83608912-13
GGGGCGG12900.015.83582810-11
CGGGTAG12100.015.70650514-15
GGGGTCG11950.015.50943140-41
GGGCCCG3250.015.34865644-45
Produced by FastQC (version 0.11.2)