FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l04n02 tsrexp_lib#23.3420000000cc75.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l04n02 tsrexp_lib#23.3420000000cc75.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10468369
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCCC3950.025.85800744-45
GGGCGCC14250.024.33780742-43
GGGGGCG13400.022.87518540-41
GGGGCGC13350.020.9963742-43
GGGGCCC4650.020.94375844-45
TCGGGGG23300.018.96966438-39
GGGGGGC15650.017.46076440-41
CTCGGGG19500.014.622836-37
GGGCCCG3300.014.39593144-45
AGGGTAG28000.014.33299528-29
GTAGGGG15400.014.03778218-19
GCGCCCG7400.013.80258644-45
CGGGGGG20350.013.7790838-39
GAGCGGC22950.013.6613029
GGGTAGA31100.013.43880228-29
CGGGTAG10850.012.91845114-15
CGGGGGT9500.012.50688238-39
GGGGTCG11900.010.78267640-41
GAGGGGC18550.010.7556399
GCGGGCT12600.010.5508011
Produced by FastQC (version 0.11.2)