FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l04n02 tsrexp_lib#17.3420000000cc0b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l04n02 tsrexp_lib#17.3420000000cc0b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12286181
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG335680.2732175278876325Illumina Single End PCR Primer 1 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGCC36150.036.73227342-43
GGCGCCC10250.036.38243544-45
GGGGCGC32800.034.1831842-43
TCGGGGG54500.033.78955538-39
GGGGGCG36900.031.68128240-41
GAGCGGC33300.030.2419139
CTCGGGG43350.029.1601136-37
GTAGGGG32700.028.98545518-19
CGGGTAG22900.028.21738614-15
AGGGTAG54350.028.13875228-29
GGGCCCG7150.027.57325644-45
GGGTAGA58350.027.2679528-29
CGGGGGT19950.026.6797938-39
GGGGGGC45050.025.05317740-41
GCGCCCG12500.024.89300244-45
GATCGGG49400.024.4124741
GAGGGGC24200.023.9476079
AGAGCGG45550.023.5609288
TAGGGGA40850.023.14440318-19
GGGGCCC13600.023.05424944-45
Produced by FastQC (version 0.11.2)