FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l04n02 tsrexp_lib#12.3420000000cbb9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l04n02 tsrexp_lib#12.3420000000cbb9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9082215
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG97830.10771601421018992Illumina Single End PCR Primer 1 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCCC4850.024.48773644-45
GGGGCGC13600.024.10414342-43
TCGGGGG23300.023.15236338-39
GTAGGGG13200.020.51772318-19
AGGGTAG23850.020.01276228-29
GGGGGCG18050.019.2209440-41
GGGCGCC21500.018.67238442-43
GGGTAGA27850.017.73525628-29
GGGGGGC18600.017.6305240-41
CTCGGGG23500.017.19035336-37
CGGGTAG10750.016.35453814-15
CGGGGGG21750.015.40583338-39
CGGGGGT10600.015.02084338-39
GGGGTCG12000.013.86167740-41
ATTAAAA81400.013.82631954-55
TTAAAAA120700.013.67080956-57
TAGGGGA21400.013.43290318-19
CATTAAA81750.013.41858954-55
GCGGGCT8150.013.3987841
GGGTAGG14100.013.31130516-17
Produced by FastQC (version 0.11.2)