FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l04n01 tsrexp_lib#9.3410000000cb1c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l04n01 tsrexp_lib#9.3410000000cb1c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19586394
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC438260.22375736952907205TruSeq Adapter, Index 5 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAC22700.018.6174268
CGGGCCC4900.017.4693851
GGCCGTA25950.015.3708347
CGCGTAT16900.015.1952651
CGGGGGT11950.014.3263581
GCGGGGT8350.013.6686621
CGGGGCG3902.188337E-512.1937311
GCCCCGG9900.012.0089781
CGCCAAT63300.011.6447241
CGGGCCT13500.011.624691
CCGGGTT82750.011.32138251
CGCGGAT30100.011.21742251
CCGGGAT63650.010.833551
CGGGGCT25900.010.8331181
CGCGACT17650.010.7774611
CGGGGTT38000.010.7625711
CTGGGGT32700.010.76180651
TGAAAAA223500.010.72195262-63
GCGGACT11600.010.6590021
CGGGCAT29950.010.6384711
Produced by FastQC (version 0.11.2)