FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l04n01 tsrexp_lib#6.3410000000caa1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l04n01 tsrexp_lib#6.3410000000caa1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8903821
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC188770.2120101021797271TruSeq Adapter, Index 4 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGGGT4200.022.6490481
GCCGTAC10850.016.6306938
GGCCGTA12800.014.0971127
CGGGCGT5905.5661076E-1012.898441
CGCGTAT8650.012.6468091
CCGGGTT39100.012.4077381
CGGGCAT15550.012.2348561
CGCGGGT10700.012.0018771
GCCCCGG5202.3658868E-711.8907491
CGGGGGT5202.3658868E-711.8907491
CCCCGAT7301.07320375E-1011.7278631
GCGGGCT7401.364242E-1011.5693791
CGCGCAA5807.847848E-811.4807231
CGCCAAT30150.011.2005741
CGGGGCT12450.011.0789321
CGGGGCA7351.4988473E-911.0009661
CCCGGGA17350.010.9655331
CGGGGTT19150.010.9283131
CGCCCAT19650.010.8922891
CCCCCTT10200.010.724991
Produced by FastQC (version 0.11.2)