FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l04n01 tsrexp_lib#40.3410000000ce69.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l04n01 tsrexp_lib#40.3410000000ce69.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7484917
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGAT12850.025.5351871
CGCGCCT9150.018.710031
CCGGGGT5900.018.5382581
CGCGTAT6350.016.475631
CGCGCGT7500.015.8515531
GCCGGGT11250.015.6402011
CGGGCAT12000.015.4552631
GCGGGGT5207.4578566E-1114.6322031
CCGGCCT13950.014.3175331
GCGGGTT17400.014.2117381
GCGGATG25600.013.3568772
CGGGGAT21550.013.2402751
CCGGGTT27900.013.1244051
CGCGGCT12400.013.0391811
CCGGGAT21900.013.0286741
CGGATGG23350.013.0163153
CCCGGGT7800.012.8031771
CGGGCGA6058.3127816E-1012.5764391
CCCGTGT9900.012.4891021
GCGGGAT12950.012.4853931
Produced by FastQC (version 0.11.2)