FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l04n01 tsrexp_lib#37.3410000000ce0c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l04n01 tsrexp_lib#37.3410000000ce0c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8416836
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC149580.1777152364617773TruSeq Adapter, Index 10 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGAT15600.028.6568151
CGCGCCT11850.017.658711
CGCGGCT17850.017.3180831
CCGCCCT9000.015.3242821
CCGGGGT5950.015.1866251
CGGGCAT14750.015.1541131
CGGGGGT7000.014.9468371
CCGGGTT30900.014.3136081
GCGGGTT19300.014.0456611
TCGGGCG11600.013.9178367
CCCGGGT8950.013.8157651
CGCGCGT10750.013.2720331
CGCGACT9700.013.2378271
CGGGTGT11550.013.1762751
CCGGAAC38100.013.1065681
CCGGCCT17500.013.0445121
CGCGCAT9150.012.9940211
GTCCTAT20550.012.94616542-43
GCGGATG31700.012.8832692
CGGATGG28250.012.6072973
Produced by FastQC (version 0.11.2)