FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l04n01 tsrexp_lib#34.3410000000cdc7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l04n01 tsrexp_lib#34.3410000000cdc7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7233938
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC174450.2411549559866286TruSeq Adapter, Index 9 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGAT14100.029.3415241
CGGATGG24200.017.2689953
GCGGATG26850.015.5652512
CGCGTAT5651.8189894E-1215.149791
CCCGGGT7350.014.8806891
ATGGACG26150.014.1637296
CGCGGGT7000.013.5867171
CGCGGCT15550.013.4556551
CGGGAGT14600.012.7026481
CGCGCCT7501.8189894E-1212.6809351
CGGGCAT12400.012.6553681
GCGGGTT17850.012.2546851
GGGGGTC2800.003518165611.8729272
GCGGGAT12650.011.6534291
CCCGACT13600.011.5387181
CCGGGCT11500.011.1647361
CCGGGTT30800.011.1164051
CGGGGGT5656.9429916E-710.9415151
CCGCGGT10900.010.9067671
CGGGTGT9255.456968E-1210.7959311
Produced by FastQC (version 0.11.2)