FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l04n01 tsrexp_lib#31.3410000000cd5e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l04n01 tsrexp_lib#31.3410000000cd5e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10000442
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC288160.28814726389093603TruSeq Adapter, Index 8 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGAT18300.026.5068341
CCGGGGT7800.022.5587941
CCCGGGT10550.017.5800841
CGGGGGT7900.015.6514671
CCGGGAT28850.015.0004531
CGCGCGT9850.014.9669841
CCGCCCT9600.013.8706111
CCCGTGT12350.013.862591
CCGGCCT20800.013.7181861
CCCGACT23600.013.7026861
CGGGACT16450.013.2984411
CGGATGG30500.012.7676863
GCGGATG35050.012.4654742
CGCGGCT18800.012.3950141
CGCGCCT12700.012.357171
CCCGGCT20500.012.2950651
CCGGCAT34050.012.2906361
CGCGGGT10500.012.2287841
CGGGGTG6301.6079866E-912.0611422
GCCGGGT13550.011.9329661
Produced by FastQC (version 0.11.2)