FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l04n01 tsrexp_lib#3.3410000000ca2a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l04n01 tsrexp_lib#3.3410000000ca2a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8566489
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC576670.6731696030894337TruSeq Adapter, Index 2 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAAAAA140550.021.66311562-63
CTGCTTG160050.019.41194756-57
GCCGTCT165600.018.2473448-49
GAAAAAA167000.018.2320462-63
TTGAAAA168600.018.08756460-61
TGCTTGA174200.017.71107958-59
TGCCGTC173650.017.45615848-49
CCGTCTT178850.017.06911750-51
ATGCCGT177350.017.04989846-47
TATGCCG179050.016.91454946-47
CTTGAAA184100.016.86143160-61
GCGGGGT4852.5465852E-1115.6855381
GCTTGAA200800.015.47134358-59
GTATGCC198100.015.34785144-45
TTCTGCT211050.014.79097454-55
CGTATGC209950.014.52684244-45
CTTCTGC214950.014.45629654-55
CGTCTTC224600.013.86723350-51
TCTGCTT233350.013.65016756-57
CGCGCTA3901.4900379E-613.4106341
Produced by FastQC (version 0.11.2)