FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l04n01 tsrexp_lib#26.3410000000cce2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l04n01 tsrexp_lib#26.3410000000cce2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9130119
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC377800.4137952637857185TruSeq Adapter, Index 3 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGAT19850.022.5219211
CCGGGGT7650.021.1375941
CGGGGGT7950.020.9381831
CCCGTGT14100.018.5516131
CCGGGCT12600.018.4953961
GCCGGGT12700.018.3497641
CGGGGGA13900.017.4499231
GCGGGTT21000.016.9855691
CGCGGCT19350.016.7134481
CCCGGGT10550.016.6796681
CGGGTGT11600.016.3998581
CCGGGTT36650.015.8315691
GCGGGGT5500.015.5649571
CCGCGGT15850.015.3030841
CCCCTGT14100.014.1666871
CGCGCGT11100.014.13933851
CCGGGGA20700.014.0151461
CCCCCAT23600.013.9051341
CCCCGGT12350.013.8635251
CCCCCCT5853.274181E-1113.8207351
Produced by FastQC (version 0.11.2)