FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l04n01 tsrexp_lib#23.3410000000cc78.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l04n01 tsrexp_lib#23.3410000000cc78.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10468369
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC261950.250230002400565TruSeq Adapter, Index 1 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGAT22850.022.0617581
CGGGGGA14300.019.6216931
CCGGGGT8300.018.9084661
CCCCCGT5400.018.4946441
CCCCCCT5650.017.6762981
GCGGGTT24100.017.5627861
CCCCCAT27250.016.0561641
GCCGGGT17300.015.6692871
CCCGGGT12150.015.6568421
CGCGCCT15900.015.5534481
CGGGTGT13900.015.0542231
CCCCCTT13150.014.8278631
CCCGTGT15250.014.6571151
CGGGGAT26300.014.46620751
CTCGGGA42600.014.4012641
GCGGATG38400.013.8513712
CGCGGGT7950.013.758821
GCGGGAT18750.013.6966071
CCCGGGA18500.013.6246261
CGCGCAT11250.013.5275121
Produced by FastQC (version 0.11.2)