FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l04n01 tsrexp_lib#17.3410000000cc0e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l04n01 tsrexp_lib#17.3410000000cc0e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12286181
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC1097930.8936300059391931TruSeq Adapter, Index 7 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAAAAA249650.021.80431662-63
CCGGGGT10050.021.7671321
GCCGTCT251800.021.46347248-49
CTGCTTG262000.021.11350356-57
TGCCGTC257300.021.05083848-49
ATGCCGT258350.020.97127346-47
TTGAAAA271050.020.21441360-61
CGGGGGA16000.020.2114931
TATGCCG267100.020.13309546-47
CGCGGAT25400.020.0336251
GAAAAAA277100.019.89289562-63
GCTTGAA281100.019.55158-59
CCCGGGT13400.019.519441
CTTGAAA288200.019.05271160-61
TGCTTGA291450.018.8811558-59
GTATGCC291700.018.56458544-45
CGTATGC296650.018.17474444-45
CCGTCTT304050.018.1431550-51
GCGGGTT29500.017.7329161
TCGTATG323400.016.79726642-43
Produced by FastQC (version 0.11.2)