FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l04n01 tsrexp_lib#12.3410000000cbbc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l04n01 tsrexp_lib#12.3410000000cbbc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9082215
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC312150.34369369146182954TruSeq Adapter, Index 6 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGT5950.015.98374751
TGAAAAA114300.015.06425262-63
GCCGTCT111750.015.0510248-49
TGCCGTC113150.014.86479448-49
TATGCCG114750.014.42777246-47
ATGCCGT117050.014.30661646-47
GCCGTAC11750.013.7403858
CTGCTTG130300.013.23454656-57
GTATGCC130950.012.64268444-45
GGCGCGC4700.012.63689238-39
GAAAAAA138900.012.61856462-63
CCGTCTT136000.012.57770250-51
TTGAAAA141900.012.10113460-61
GCCCCGG5202.3732537E-711.8879131
CGTATGC140150.011.84666444-45
TGCTTGA146350.011.78209358-59
GGCCGTA13150.011.5553247
CCGGGTT40550.011.4921381
CGCGCAT6352.2839231E-811.2326741
CGCGTAT8207.094059E-1111.0180661
Produced by FastQC (version 0.11.2)