##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C5B16ACXX l03n02 tsrexp_lib#8.3420000000cb26.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 10593195 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.62705774792213 33.0 33.0 33.0 33.0 33.0 2 32.59015821005844 33.0 33.0 33.0 33.0 33.0 3 32.579476258107206 33.0 33.0 33.0 33.0 33.0 4 36.46913070136064 37.0 37.0 37.0 37.0 37.0 5 36.46873639161745 37.0 37.0 37.0 37.0 37.0 6 36.466044474778386 37.0 37.0 37.0 37.0 37.0 7 36.496389804964416 37.0 37.0 37.0 37.0 37.0 8 36.4989848671718 37.0 37.0 37.0 37.0 37.0 9 36.48206409869732 37.0 37.0 37.0 37.0 37.0 10-11 36.47028790652867 37.0 37.0 37.0 37.0 37.0 12-13 36.46967968587381 37.0 37.0 37.0 37.0 37.0 14-15 38.85935834278516 40.0 40.0 40.0 37.0 40.0 16-17 38.847386553348635 40.0 40.0 40.0 37.0 40.0 18-19 38.83976637832117 40.0 40.0 40.0 37.0 40.0 20-21 38.81552817634339 40.0 40.0 40.0 37.0 40.0 22-23 38.79757825660719 40.0 40.0 40.0 37.0 40.0 24-25 38.77316668861472 40.0 40.0 40.0 37.0 40.0 26-27 38.75723414890408 40.0 40.0 40.0 37.0 40.0 28-29 38.720905874006846 40.0 40.0 40.0 37.0 40.0 30-31 38.70620015963078 40.0 40.0 40.0 37.0 40.0 32-33 38.67232591300359 40.0 40.0 40.0 37.0 40.0 34-35 38.63605276783822 40.0 40.0 40.0 37.0 40.0 36-37 38.607430524973815 40.0 40.0 40.0 37.0 40.0 38-39 38.56655650160315 40.0 40.0 40.0 37.0 40.0 40-41 38.511726584849995 40.0 40.0 40.0 37.0 40.0 42-43 38.452813433529734 40.0 38.5 40.0 37.0 40.0 44-45 38.390684160916514 40.0 37.0 40.0 37.0 40.0 46-47 38.308438672185304 40.0 37.0 40.0 37.0 40.0 48-49 38.20471826488609 40.0 37.0 40.0 37.0 40.0 50-51 36.847491007198485 38.5 37.0 38.5 35.0 40.0 52-53 37.164219576813224 38.5 37.0 40.0 33.0 40.0 54-55 37.796837356434956 40.0 37.0 40.0 37.0 40.0 56-57 37.735706507809965 40.0 37.0 40.0 37.0 40.0 58-59 37.55618158638636 40.0 37.0 40.0 33.0 40.0 60-61 37.34047154800794 37.0 37.0 40.0 33.0 40.0 62-63 37.116958906165706 37.0 37.0 40.0 33.0 40.0 64-65 36.86430859622617 37.0 37.0 40.0 33.0 40.0 66-67 36.60421147727385 37.0 37.0 40.0 33.0 40.0 68-69 36.336178178538205 37.0 37.0 37.0 33.0 40.0 70-71 36.09371884497548 37.0 37.0 37.0 33.0 40.0 72-73 35.88224577193189 37.0 37.0 37.0 33.0 40.0 74-75 35.67929859688225 37.0 37.0 37.0 33.0 37.0 76-77 35.5067595753689 37.0 37.0 37.0 33.0 37.0 78-79 35.38079063965121 37.0 37.0 37.0 33.0 37.0 80-81 35.28436770020754 37.0 37.0 37.0 33.0 37.0 82-83 35.20051098842228 37.0 37.0 37.0 33.0 37.0 84-85 35.134220365055114 37.0 37.0 37.0 33.0 37.0 86-87 35.07608568519696 37.0 35.0 37.0 33.0 37.0 88-89 35.01032233429102 37.0 33.0 37.0 33.0 37.0 90-91 34.93839441263943 37.0 33.0 37.0 33.0 37.0 92-93 34.896509174049946 37.0 33.0 37.0 33.0 37.0 94-95 34.83733915971527 37.0 33.0 37.0 33.0 37.0 96-97 34.78526148154546 37.0 33.0 37.0 33.0 37.0 98-99 34.726477752934784 37.0 33.0 37.0 33.0 37.0 100-101 32.87351044703699 35.0 30.0 37.0 27.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.17752912473950033 2306 2 0.1924253164374008 2306 3 0.1778933225689272 2306 4 0.2375669865251524 2306 5 0.22882770072901337 2306 6 0.24991442265110209 2306 7 0.18003479513996012 2306 8 0.2278871962760789 2306 9 0.22165496414054786 2306 10-11 0.20176806330383812 2306 12-13 0.2058339474911861 2306 14-15 0.3942591254537646 2306 16-17 0.43715729191956143 2306 18-19 0.43526560564273353 2306 20-21 0.42839325020248253 2306 22-23 0.46182496992920363 2306 24-25 0.48327597704547287 2306 26-27 0.4611554404115381 2306 28-29 0.4750837547893312 2306 30-31 0.44176235038946743 2306 32-33 0.4813829327626351 2306 34-35 0.5320222820935712 2306 36-37 0.5425259343809827 2306 38-39 0.49416506033802676 2306 40-41 0.4897125744956199 2306 42-43 0.5448862827982595 2306 44-45 0.5070736928516553 2306 46-47 0.495303078705831 2306 48-49 0.529419068794688 2306 50-51 0.18748888402629404 2306 52-53 0.2637155517816936 2306 54-55 0.47630345961238874 2306 56-57 0.5087429733945399 2306 58-59 0.5362346517917231 2306 60-61 0.5716693682873526 2306 62-63 0.5804861721536554 2306 64-65 0.6178761974512028 2306 66-67 0.5768949156593663 2306 68-69 0.5445582898790775 2306 70-71 0.5592738000793958 2306 72-73 0.5705194890305876 2306 74-75 0.5597183993085153 2306 76-77 0.5214192284087531 2306 78-79 0.524623209794953 2306 80-81 0.554728423010431 2306 82-83 0.6088871093946508 2306 84-85 0.6146372318889277 2306 86-87 0.6475804104493932 2306 88-89 0.6861567123756345 2306 90-91 0.7186755080860294 2306 92-93 0.7124366577593193 2306 94-95 0.7034718927269736 2306 96-97 0.6782241970527494 2306 98-99 0.6645526984588486 2306 100-101 0.5598886025965797 2307 1 0.1287993545453432 2307 2 0.18705058173509315 2307 3 0.19194587390551732 2307 4 0.2154362233269893 2307 5 0.18749930194796605 2307 6 0.22966176601806865 2307 7 0.17513764198298531 2307 8 0.18365411911536 2307 9 0.22222040697975132 2307 10-11 0.20909351296172218 2307 12-13 0.222629736364496 2307 14-15 0.3705995300407423 2307 16-17 0.3786292736498993 2307 18-19 0.3765202763445501 2307 20-21 0.41018699621502464 2307 22-23 0.44063497382298067 2307 24-25 0.4285834070260748 2307 26-27 0.40228031861681757 2307 28-29 0.4235903527269116 2307 30-31 0.41835091457367923 2307 32-33 0.4347077914990365 2307 34-35 0.4926487114105882 2307 36-37 0.4540057950642833 2307 38-39 0.474861568462849 2307 40-41 0.5276613411926974 2307 42-43 0.48166391214745374 2307 44-45 0.45158128799777586 2307 46-47 0.4716634777354969 2307 48-49 0.4560507752735319 2307 50-51 0.4172995812130935 2307 52-53 0.45723445030041177 2307 54-55 0.4671067014999011 2307 56-57 0.48922411280813805 2307 58-59 0.45679838767678405 2307 60-61 0.49366373511019646 2307 62-63 0.4832018973372314 2307 64-65 0.4601328982173385 2307 66-67 0.48658293151598286 2307 68-69 0.5008147909293044 2307 70-71 0.5750640398664615 2307 72-73 0.5872607216388488 2307 74-75 0.5307401678866697 2307 76-77 0.5397613303525333 2307 78-79 0.5524939775998305 2307 80-81 0.5504449278630261 2307 82-83 0.585226828457877 2307 84-85 0.5678362398903545 2307 86-87 0.5750229772696756 2307 88-89 0.5793843889802588 2307 90-91 0.5965300289780444 2307 92-93 0.5983771026550926 2307 94-95 0.6334073095345545 2307 96-97 0.6142596326893894 2307 98-99 0.6179154895093788 2307 100-101 0.6602934386467183 2201 1 0.22031806586080904 2201 2 0.21222388094746947 2201 3 0.21233195175045694 2201 4 0.27386811309205683 2201 5 0.29221613365149324 2201 6 0.2903529350471743 2201 7 0.2649755168085264 2201 8 0.27659046494564166 2201 9 0.2830567999545224 2201 10-11 0.28804667346463475 2201 12-13 0.2978842418403076 2201 14-15 0.48772820528997585 2201 16-17 0.496027268794343 2201 18-19 0.5311373611284651 2201 20-21 0.5376670170445692 2201 22-23 0.5274146478672108 2201 24-25 0.5553427105865794 2201 26-27 0.5295822189041175 2201 28-29 0.5744700453906404 2201 30-31 0.5587129097153252 2201 32-33 0.5405595776864018 2201 34-35 0.49870739426648214 2201 36-37 0.5746836962374715 2201 38-39 0.5830167442641354 2201 40-41 0.5931573114837292 2201 42-43 0.6175698274723942 2201 44-45 0.6271231488220153 2201 46-47 0.5846443783774404 2201 48-49 0.5705563667312603 2201 50-51 0.552780425202215 2201 52-53 0.599223432554183 2201 54-55 0.6459359295871749 2201 56-57 0.640634969426749 2201 58-59 0.628055806275448 2201 60-61 0.6815617065422188 2201 62-63 0.6555655789806423 2201 64-65 0.6014027255348822 2201 66-67 0.6250167305393575 2201 68-69 0.6309358550771051 2201 70-71 0.6357926756806691 2201 72-73 0.6572982766154425 2201 74-75 0.6822802600917512 2201 76-77 0.7288005640046364 2201 78-79 0.7152266512997087 2201 80-81 0.6955906984791511 2201 82-83 0.6711538192144744 2201 84-85 0.7312508645636981 2201 86-87 0.7890064458611192 2201 88-89 0.7769272833387575 2201 90-91 0.7120167946465799 2201 92-93 0.6961284852174217 2201 94-95 0.7826351637735129 2201 96-97 0.8630925408735095 2201 98-99 0.7950435269764 2201 100-101 0.7155161653969841 1101 1 0.18403576417871648 1101 2 0.19409747945596934 1101 3 0.18606947702515697 1101 4 0.2565743666553715 1101 5 0.22007603061445025 1101 6 0.23110744127994565 1101 7 0.2086597418926459 1101 8 0.23040696960315188 1101 9 0.2212816458838347 1101 10-11 0.23155059892420837 1101 12-13 0.23306593301171574 1101 14-15 0.40372062236205153 1101 16-17 0.40187159703349096 1101 18-19 0.3960793295253282 1101 20-21 0.4030838761870541 1101 22-23 0.385469857632458 1101 24-25 0.4269262791624655 1101 26-27 0.46221376669269176 1101 28-29 0.41420248938033666 1101 30-31 0.35156547197360766 1101 32-33 0.4083302031576679 1101 34-35 0.4617139313059013 1101 36-37 0.4944299823959497 1101 38-39 0.4915161469729341 1101 40-41 0.49135249934433745 1101 42-43 0.5075425785918242 1101 44-45 0.5185151704684969 1101 46-47 0.4808082679395156 1101 48-49 0.5045545215684797 1101 50-51 0.44047544257056614 1101 52-53 0.5110885219011436 1101 54-55 0.5679753373462617 1101 56-57 0.5461588856055997 1101 58-59 0.5036732134092645 1101 60-61 0.4575027923022432 1101 62-63 0.414995080780308 1101 64-65 0.45728400887591647 1101 66-67 0.4335648993226897 1101 68-69 0.41790340132347126 1101 70-71 0.4677069555769364 1101 72-73 0.43004018376227293 1101 74-75 0.479795979258931 1101 76-77 0.5071335473122716 1101 78-79 0.4916955977637514 1101 80-81 0.5065225664703519 1101 82-83 0.4932942223464991 1101 84-85 0.5066289082707556 1101 86-87 0.5296436673094433 1101 88-89 0.5575091401983485 1101 90-91 0.6302832677098351 1101 92-93 0.6246312495009292 1101 94-95 0.5704909784229955 1101 96-97 0.5552923237375467 1101 98-99 0.6153032794953432 1101 100-101 0.6782980015232027 2304 1 0.1399673309788625 2304 2 0.15751064227192302 2304 3 0.17261533937865892 2304 4 0.2189219134160041 2304 5 0.20077194069254034 2304 6 0.22523050170762104 2304 7 0.1922914465455463 2304 8 0.1869103848779119 2304 9 0.22832979886500482 2304 10-11 0.20443844900795227 2304 12-13 0.211192581867941 2304 14-15 0.36110044863441004 2304 16-17 0.3776779851089387 2304 18-19 0.3794777616280456 2304 20-21 0.3645167652471528 2304 22-23 0.36997523927575315 2304 24-25 0.38715261850556004 2304 26-27 0.40096268617188713 2304 28-29 0.40484104292707457 2304 30-31 0.3874890625282177 2304 32-33 0.4004442386569522 2304 34-35 0.42267480725124074 2304 36-37 0.4361239861948647 2304 38-39 0.46426056457119813 2304 40-41 0.49972517592328103 2304 42-43 0.4840213266540587 2304 44-45 0.522876970284841 2304 46-47 0.4897166041450234 2304 48-49 0.4762007208104606 2304 50-51 0.37264898652946243 2304 52-53 0.42899419359614654 2304 54-55 0.500221788163941 2304 56-57 0.502193668527191 2304 58-59 0.5092404458213977 2304 60-61 0.5109560727052056 2304 62-63 0.5063302358443735 2304 64-65 0.5159750885218344 2304 66-67 0.4927846712865005 2304 68-69 0.5180598453314147 2304 70-71 0.5093642002080188 2304 72-73 0.4852961744424249 2304 74-75 0.47410556953295924 2304 76-77 0.5257129783353918 2304 78-79 0.5663738580320654 2304 80-81 0.48566350260148283 2304 82-83 0.5373588926117137 2304 84-85 0.5228723212242627 2304 86-87 0.5842570487048562 2304 88-89 0.60306719575361 2304 90-91 0.6256929578074804 2304 92-93 0.6418008441423311 2304 94-95 0.6624410499246309 2304 96-97 0.6024422182401494 2304 98-99 0.6262788779224806 2304 100-101 0.5791667514210488 2202 1 0.19179314846004303 2202 2 0.18311024107124751 2202 3 0.18949135694253272 2202 4 0.24065904054511122 2202 5 0.22875948984663808 2202 6 0.2584123636237621 2202 7 0.22617043809812287 2202 8 0.2439180597524384 2202 9 0.244916834207757 2202 10-11 0.23338105993378377 2202 12-13 0.218019678630327 2202 14-15 0.4097200598721358 2202 16-17 0.42068126742543654 2202 18-19 0.43462853536791357 2202 20-21 0.39409560700669743 2202 22-23 0.4130929784804067 2202 24-25 0.43308553604511957 2202 26-27 0.43862177612293607 2202 28-29 0.46478619725686343 2202 30-31 0.471481982719979 2202 32-33 0.49930049483318584 2202 34-35 0.4732826575114899 2202 36-37 0.46253319279353633 2202 38-39 0.4799333405167161 2202 40-41 0.4664703750146657 2202 42-43 0.5429366428319184 2202 44-45 0.5105873639879164 2202 46-47 0.5074197995283427 2202 48-49 0.49747089528349875 2202 50-51 0.5033872058088988 2202 52-53 0.4970732337052297 2202 54-55 0.4794829897540325 2202 56-57 0.4746824356396715 2202 58-59 0.5136188259302017 2202 60-61 0.533524273269073 2202 62-63 0.5047778463905459 2202 64-65 0.5300144444481845 2202 66-67 0.48627540726593566 2202 68-69 0.4747933748906945 2202 70-71 0.49859471424303337 2202 72-73 0.5399744126942991 2202 74-75 0.576438498959007 2202 76-77 0.5393648044567456 2202 78-79 0.5256410922607344 2202 80-81 0.5453400361462286 2202 82-83 0.5740930963509427 2202 84-85 0.5609143386773923 2202 86-87 0.60394323595537 2202 88-89 0.6338790836804549 2202 90-91 0.5946408756037371 2202 92-93 0.6170037332649159 2202 94-95 0.684626570327076 2202 96-97 0.6530604391407238 2202 98-99 0.6211318778943209 2202 100-101 0.6825114454640158 1102 1 0.15582490712137087 1102 2 0.16850751629755223 1102 3 0.14337295511032977 1102 4 0.20099056996360076 1102 5 0.20336018943328327 1102 6 0.1738745951498828 1102 7 0.1906273397015994 1102 8 0.1564157750147359 1102 9 0.19573477229407388 1102 10-11 0.17757800272529067 1102 12-13 0.20628169005176744 1102 14-15 0.3421287423001118 1102 16-17 0.30652660971871626 1102 18-19 0.32056253128990164 1102 20-21 0.34404417963078515 1102 22-23 0.3408319759622387 1102 24-25 0.3433424149248836 1102 26-27 0.32981582637406603 1102 28-29 0.3682195144182856 1102 30-31 0.363249732576989 1102 32-33 0.3504993436896342 1102 34-35 0.3752672310643135 1102 36-37 0.3968361854305016 1102 38-39 0.407782051536536 1102 40-41 0.3899365411808233 1102 42-43 0.3952738720416846 1102 44-45 0.36530729765492964 1102 46-47 0.4174619436835272 1102 48-49 0.4208402240419886 1102 50-51 0.391280839014982 1102 52-53 0.4193822739719124 1102 54-55 0.4345525640433934 1102 56-57 0.40660516543275094 1102 58-59 0.39462516097042766 1102 60-61 0.3829974343472955 1102 62-63 0.3061415136918839 1102 64-65 0.349819498085175 1102 66-67 0.3726764273544916 1102 68-69 0.39207644553744814 1102 70-71 0.35834213657933134 1102 72-73 0.44337815213570053 1102 74-75 0.4449697088299587 1102 76-77 0.4538431055685024 1102 78-79 0.4486759040840056 1102 80-81 0.4906140724100254 1102 82-83 0.5094865834976474 1102 84-85 0.49034658308775647 1102 86-87 0.5296984027074672 1102 88-89 0.5197594754526662 1102 90-91 0.5026269268177757 1102 92-93 0.5615967423262234 1102 94-95 0.523809253698964 1102 96-97 0.5685790504617572 1102 98-99 0.5537905910731027 1102 100-101 0.6128330097597185 2305 1 0.1638191170790506 2305 2 0.15558865995967608 2305 3 0.16070788093941957 2305 4 0.20024570235726458 2305 5 0.19359739446025515 2305 6 0.20156412971611815 2305 7 0.1744693105434436 2305 8 0.220508461511848 2305 9 0.2133737572858081 2305 10-11 0.20053511750693787 2305 12-13 0.2122544061663234 2305 14-15 0.3735181789595501 2305 16-17 0.3366479277777614 2305 18-19 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18 12081.0 19 13618.0 20 15310.0 21 16919.0 22 19308.0 23 21829.0 24 24902.0 25 28855.0 26 33783.0 27 40223.0 28 48221.0 29 59636.0 30 75502.0 31 98753.0 32 132584.0 33 183575.0 34 261596.0 35 396745.0 36 833818.0 37 4012438.0 38 4171994.0 39 2079.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 23.12889366303284 14.678053907432966 21.732428199499605 40.46062423003459 2 25.795541024800727 24.47828281162166 30.596553800095606 19.129622363482014 3 24.04443736994864 26.385054682302734 30.099341422834442 19.47116652491419 4 25.522767128200684 29.926692311611703 22.169419419253824 22.381121140933793 5 28.094713860438596 33.05563338502218 21.87525404861323 16.974398705925992 6 19.33195466656333 37.365114500858674 22.344306903320994 20.958623929257 7 18.456426046312398 15.785938307664345 43.49608238045185 22.2615532655714 8 22.485181710591156 21.470627208188343 28.59116567770955 27.45302540351095 9 24.639498385194354 22.653423076999417 26.96764651422871 25.739432023577514 10-11 24.377578100190135 30.938553426489264 22.134880731228275 22.54898774209233 12-13 22.84658209389749 25.00793974093341 28.174783722968854 23.970694442200244 14-15 23.245308391719924 26.547867455938608 27.992937356763413 22.21388679557806 16-17 24.06285691676294 26.541283609590444 27.068122946543127 22.327736527103493 18-19 23.823207589437764 26.827185920699893 26.958081003398497 22.391525486463845 20-21 23.855812054342255 27.0926618187269 26.821905714609283 22.22962041232156 22-23 23.95817969553053 26.930300627106966 26.967967889037393 22.143551788325116 24-25 23.54023064358195 27.330816323076867 26.982578115228 22.146374918113178 26-27 23.9332345434581 27.457632902766594 26.58320023515967 22.02593231861564 28-29 24.016976452928784 26.816941547789554 26.98211697778598 22.183965021495684 30-31 23.58444130623202 27.096653535410187 27.035968863182784 22.28293629517501 32-33 23.79870534017641 27.145640770981277 26.922479291803743 22.133174597038572 34-35 24.0736764417779 26.775928984534904 27.009513582442658 22.140880991244536 36-37 23.673095471068113 26.997717180942978 27.148739240883497 22.18044810710542 38-39 24.068044285940353 27.089565797904513 26.88940690728021 21.95298300887492 40-41 24.15844139228111 26.64572824346229 26.953421598620103 22.2424087656365 42-43 23.85993424904386 26.824210854982244 27.134869822892192 22.1809850730817 44-45 24.26264554924942 26.942185259164024 26.80821985337632 21.986949338210234 46-47 24.21116582857575 26.849734394495066 26.87633747967787 22.062762297251314 48-49 24.04682844185904 26.812331937187377 27.00491830052615 22.135921320427432 50-51 24.31852035673489 27.06258654676511 26.534164802952088 22.08472829354791 52-53 24.45122031905422 26.868337678000607 26.76500611530035 21.915435887644826 54-55 24.093932344163623 26.990662069824534 26.909468224018468 22.00593736199338 56-57 24.486531452786448 27.093244922392053 26.742161455869955 21.67806216895155 58-59 24.7714151442916 27.025373744762682 26.636688170000376 21.566522940945337 60-61 24.32416289224131 27.133725587837148 26.56931138783536 21.97280013208618 62-63 25.033568281775974 27.19009326168509 26.23811904416659 21.53821941237234 64-65 25.073566086850303 27.111348228405387 26.38190853851448 21.43317714622983 66-67 24.971250005079504 27.29949636831973 26.3787594120854 21.35049421451536 68-69 25.491397643930007 27.295627739145782 26.098705056980847 21.11426955994336 70-71 25.618484126616654 27.327859991816915 25.95697645521369 21.096679426352736 72-73 25.60372545106239 27.345506499259887 25.89193307811056 21.158834971567156 74-75 26.012605671744065 27.460566333102037 25.664801885316113 20.862026109837785 76-77 26.325349448895015 27.352778913645366 25.70996688467287 20.611904752786753 78-79 26.320928753541452 27.371113478065407 25.697433858562757 20.610523909830384 80-81 26.77534272897475 27.502664295379585 25.23416995508445 20.487823020561212 82-83 27.081032872226736 27.442914857012603 25.22986969734658 20.246182573414085 84-85 27.074405072705854 27.4047414622068 25.311111499635913 20.209741965451432 86-87 27.44325372110233 27.55896254277767 25.01815540688053 19.979628329239468 88-89 27.800222085939613 27.391059200217466 24.95617141495832 19.852547298884605 90-91 27.887121404480496 27.413215868525732 24.967116874483736 19.732545852510036 92-93 28.396158328258576 27.51733974396384 24.531662584887684 19.554839342889895 94-95 28.6133542223966 27.462434819927523 24.625442143779654 19.29876881389622 96-97 28.56858998073613 27.62676449041988 24.64498731784509 19.159658210998906 98-99 29.136691616272042 27.615452542365432 24.35448678280122 18.893369058561305 100-101 29.348325563661465 27.555532247327392 24.236344488682832 18.859797700328315 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 8387.0 1 4597.5 2 642.5 3 417.5 4 339.5 5 298.5 6 288.0 7 286.5 8 266.5 9 270.0 10 287.5 11 306.5 12 345.0 13 401.0 14 420.0 15 460.0 16 506.0 17 556.5 18 683.0 19 825.0 20 984.0 21 1196.0 22 1509.5 23 2078.5 24 2938.0 25 4103.0 26 6198.0 27 9422.5 28 13988.0 29 20124.0 30 28139.5 31 38126.0 32 49617.5 33 63133.0 34 78975.5 35 97974.0 36 124259.0 37 157253.5 38 196612.0 39 245093.5 40 305648.0 41 375094.0 42 447651.5 43 523796.0 44 597393.5 45 657677.5 46 698937.5 47 716614.5 48 709941.0 49 682108.5 50 633058.5 51 569130.5 52 494436.0 53 418354.0 54 345137.5 55 280700.0 56 228422.0 57 186370.5 58 152576.0 59 121314.5 60 94182.0 61 66997.0 62 42825.0 63 27813.5 64 18271.5 65 11647.0 66 7972.0 67 5152.5 68 3666.5 69 2956.5 70 2552.5 71 1985.0 72 1181.5 73 876.5 74 779.0 75 616.5 76 470.5 77 225.0 78 106.0 79 90.0 80 68.0 81 47.0 82 42.5 83 39.5 84 27.5 85 20.0 86 20.5 87 18.0 88 15.5 89 10.0 90 8.5 91 12.0 92 8.5 93 4.5 94 3.5 95 2.0 96 1.0 97 1.0 98 0.5 99 0.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1320281558113487 2 0.13166943495328842 3 0.1295265498275072 4 0.13812641039837367 5 0.13698416766612906 6 0.12201229185340211 7 0.12194621169533837 8 0.1197844465243961 9 0.12540125995981383 10-11 0.09041653627635476 12-13 0.09776087384401023 14-15 0.14964323794662515 16-17 0.15162092267724706 18-19 0.1512433217740257 20-21 0.18207443552204977 22-23 0.15454260966592234 24-25 0.15194660345627548 26-27 0.14018905533222037 28-29 0.1506250002950007 30-31 0.10804577844550205 32-33 0.12238045273404294 34-35 0.1356767245387251 36-37 0.13789984985644085 38-39 0.14017489529834953 40-41 0.1719830513834589 42-43 0.152574364957881 44-45 0.13541240390647016 46-47 0.12833710698235992 48-49 0.14974235818372075 50-51 0.1254909401743289 52-53 0.12437229749853562 54-55 0.1107125848245029 56-57 0.10474649055360541 58-59 0.11589987723250633 60-61 0.11539955603573804 62-63 0.10063536071978285 64-65 0.1170798800550731 66-67 0.10812601863743657 68-69 0.11542787610347965 70-71 0.09803463449884572 72-73 0.10447272989876992 74-75 0.13579944483227205 76-77 0.16594143693191715 78-79 0.13403888062100244 80-81 0.12277221367113511 82-83 0.11311979058253908 84-85 0.11195866780513339 86-87 0.11634827830508171 88-89 0.12325365482274234 90-91 0.1399105746660946 92-93 0.11543731612606016 94-95 0.11535235592283538 96-97 0.113171710706732 98-99 0.10507217133263383 100-101 0.09798743438594304 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.0593195E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.251866389236724 #Duplication Level Percentage of deduplicated Percentage of total 1 71.83468072142695 33.22488054840919 2 14.033295497090533 12.981322166642173 3 5.147069043907048 7.1418464914489554 4 2.645788010652432 4.894905342517629 5 1.5078751063379734 3.4871018975000028 6 0.9940455302036247 2.758587662867762 7 0.7185861606418035 2.3265165763811506 8 0.4349494229598013 1.6093778077449876 9 0.3837946596606276 1.597609738757334 >10 2.0664371199792595 17.505731769499675 >50 0.15650929425884424 4.984793430571861 >100 0.07318862749203447 6.0302613459650445 >500 0.003130810308984072 0.9703194837457919 >1k 6.499950802052978E-4 0.4867457379483465 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 3.020807225770884E-4 0.0 0.0 2 4.720011290267006E-4 0.0 0.0 3 6.796816257984489E-4 0.0 0.0 4 0.0019068845612678705 0.0 0.0 5 0.002161765170942289 0.0 0.0 6 0.0027187265031937955 9.440022580534013E-6 0.0 7 0.0033134479257674386 9.440022580534013E-6 0.0 8 0.004606731019300598 9.440022580534013E-6 0.0 9 0.005201452441874241 9.440022580534013E-6 0.0 10-11 0.006461695456375531 9.440022580534013E-6 0.0 12-13 0.007485937906363472 9.440022580534013E-6 0.0 14-15 0.008835861135379836 1.416003387080102E-5 0.0 16-17 0.010417064917619283 1.8880045161068026E-5 0.0 18-19 0.01289507084500946 1.8880045161068026E-5 0.0 20-21 0.015528837144978451 2.8320067741602038E-5 0.0 22-23 0.019526686707834605 3.776009032213605E-5 0.0 24-25 0.025214300312606346 3.776009032213605E-5 0.0 26-27 0.03344127999154174 3.776009032213605E-5 0.0 28-29 0.050872281686497794 5.1920124192937067E-5 0.0 30-31 0.08198187610064764 6.608015806373809E-5 0.0 32-33 0.12489149874046498 7.55201806442721E-5 0.0 34-35 0.1791197084543426 7.55201806442721E-5 0.0 36-37 0.25407820775507295 7.55201806442721E-5 0.0 38-39 0.3549306889942081 8.496020322480611E-5 0.0 40-41 0.48393331756849567 1.0384024838587413E-4 9.440022580534013E-6 42-43 0.6525368408681234 1.0384024838587413E-4 9.440022580534013E-6 44-45 0.8619543017946899 1.0384024838587413E-4 9.440022580534013E-6 46-47 1.1138141042433374 1.0384024838587413E-4 9.440022580534013E-6 48-49 1.4243814071203258 1.0384024838587413E-4 9.440022580534013E-6 50-51 1.778726814714541 1.0384024838587413E-4 9.440022580534013E-6 52-53 2.18529442722427 1.1328027096640815E-4 9.440022580534013E-6 54-55 2.647954653907532 1.2272029354694217E-4 9.440022580534013E-6 56-57 3.1677034171465737 1.2272029354694217E-4 9.440022580534013E-6 58-59 3.7634349221363337 1.3216031612747617E-4 9.440022580534013E-6 60-61 4.432826923322001 1.3216031612747617E-4 9.440022580534013E-6 62-63 5.183185998180908 1.3216031612747617E-4 9.440022580534013E-6 64-65 6.00645980745186 1.3216031612747617E-4 9.440022580534013E-6 66-67 6.904163474759032 1.3216031612747617E-4 9.440022580534013E-6 68-69 7.884670300131358 1.3216031612747617E-4 2.8320067741602038E-5 70-71 8.943869153735015 1.4160033870801018E-4 2.8320067741602038E-5 72-73 10.080905713526466 1.4160033870801018E-4 2.8320067741602038E-5 74-75 11.294892617383141 1.4160033870801018E-4 2.8320067741602038E-5 76-77 12.578721528301894 1.4160033870801018E-4 2.8320067741602038E-5 78-79 13.940350385318121 1.4160033870801018E-4 2.8320067741602038E-5 80-81 15.373062612365768 1.4160033870801018E-4 3.776009032213605E-5 82-83 16.87073635480136 1.4160033870801018E-4 3.776009032213605E-5 84-85 18.44814524796343 1.4160033870801018E-4 3.776009032213605E-5 86-87 20.074590338420087 1.4160033870801018E-4 3.776009032213605E-5 88-89 21.756184040792228 1.4632034999827718E-4 3.776009032213605E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGGGG 1675 0.0 15.03564 38-39 GGGGCGC 930 0.0 13.537438 42-43 GGCGCCC 400 0.0 13.062959 44-45 GGGGCCC 395 3.6379788E-11 11.424452 44-45 CGAAAAT 6145 0.0 11.28538 1 CAAAAAT 8765 0.0 11.271889 1 GTACGGC 1130 0.0 10.300349 34-35 GGGGGGC 1285 0.0 9.984294 40-41 GGGCGCC 1650 0.0 9.933646 42-43 CGCGAAC 1335 0.0 9.606556 1 CGGGAAT 2830 0.0 9.566953 1 GGGGGCG 1480 0.0 9.471462 40-41 CCGGGAT 2925 0.0 9.418622 1 CGGATAT 3855 0.0 9.364282 1 AGGGTAG 2075 0.0 9.271322 28-29 CTCGGCT 4075 0.0 9.208412 1 CTCGGGG 1865 0.0 9.172432 36-37 GGCCGTA 2435 0.0 9.165558 7 AAAAATC 8525 0.0 9.137784 2 CCGGTAT 3390 0.0 9.107494 1 >>END_MODULE