FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l03n02 tsrexp_lib#8.3420000000cb26.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l03n02 tsrexp_lib#8.3420000000cb26.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10593195
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGGGG16750.015.0356438-39
GGGGCGC9300.013.53743842-43
GGCGCCC4000.013.06295944-45
GGGGCCC3953.6379788E-1111.42445244-45
CGAAAAT61450.011.285381
CAAAAAT87650.011.2718891
GTACGGC11300.010.30034934-35
GGGGGGC12850.09.98429440-41
GGGCGCC16500.09.93364642-43
CGCGAAC13350.09.6065561
CGGGAAT28300.09.5669531
GGGGGCG14800.09.47146240-41
CCGGGAT29250.09.4186221
CGGATAT38550.09.3642821
AGGGTAG20750.09.27132228-29
CTCGGCT40750.09.2084121
CTCGGGG18650.09.17243236-37
GGCCGTA24350.09.1655587
AAAAATC85250.09.1377842
CCGGTAT33900.09.1074941
Produced by FastQC (version 0.11.2)