##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C5B16ACXX l03n02 tsrexp_lib#5.3420000000caba.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 13499843 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.60388013401341 33.0 33.0 33.0 33.0 33.0 2 32.549258906196165 33.0 33.0 33.0 33.0 33.0 3 32.534143026700384 33.0 33.0 33.0 33.0 33.0 4 36.415120161027055 37.0 37.0 37.0 37.0 37.0 5 36.41620706255621 37.0 37.0 37.0 37.0 37.0 6 36.4341006039848 37.0 37.0 37.0 37.0 37.0 7 36.43600936692375 37.0 37.0 37.0 37.0 37.0 8 36.4626634546787 37.0 37.0 37.0 37.0 37.0 9 36.42105993380812 37.0 37.0 37.0 37.0 37.0 10-11 36.4115021930255 37.0 37.0 37.0 37.0 37.0 12-13 36.41239979605689 37.0 37.0 37.0 37.0 37.0 14-15 38.78496538811599 40.0 40.0 40.0 37.0 40.0 16-17 38.7716117142992 40.0 40.0 40.0 37.0 40.0 18-19 38.76716284774571 40.0 40.0 40.0 37.0 40.0 20-21 38.74496229326519 40.0 40.0 40.0 37.0 40.0 22-23 38.75753677283506 40.0 40.0 40.0 37.0 40.0 24-25 38.70919869216257 40.0 40.0 40.0 37.0 40.0 26-27 38.699917213852046 40.0 40.0 40.0 37.0 40.0 28-29 38.64954010946646 40.0 40.0 40.0 37.0 40.0 30-31 38.62619976395281 40.0 40.0 40.0 37.0 40.0 32-33 38.593409678912565 40.0 40.0 40.0 37.0 40.0 34-35 38.56107256210313 40.0 40.0 40.0 37.0 40.0 36-37 38.53379061519456 40.0 40.0 40.0 37.0 40.0 38-39 38.5004737832877 40.0 40.0 40.0 37.0 40.0 40-41 38.45062390725581 40.0 40.0 40.0 37.0 40.0 42-43 38.377087977986115 40.0 37.0 40.0 37.0 40.0 44-45 38.32436929081324 40.0 37.0 40.0 37.0 40.0 46-47 38.2268662680003 40.0 37.0 40.0 37.0 40.0 48-49 38.13179619940765 40.0 37.0 40.0 37.0 40.0 50-51 36.75970120541402 38.5 37.0 38.5 35.0 40.0 52-53 37.0758979567392 38.5 37.0 40.0 33.0 40.0 54-55 37.70576517075051 40.0 37.0 40.0 35.0 40.0 56-57 37.65665215513988 40.0 37.0 40.0 33.0 40.0 58-59 37.464510957645956 38.5 37.0 40.0 33.0 40.0 60-61 37.27053310916283 37.0 37.0 40.0 33.0 40.0 62-63 37.04307435279062 37.0 37.0 40.0 33.0 40.0 64-65 36.7804801137317 37.0 37.0 40.0 33.0 40.0 66-67 36.506528446293785 37.0 37.0 40.0 33.0 40.0 68-69 36.213889524492984 37.0 37.0 37.0 33.0 40.0 70-71 35.96008327652403 37.0 37.0 37.0 33.0 40.0 72-73 35.74558959685679 37.0 37.0 37.0 33.0 38.5 74-75 35.54316783535927 37.0 37.0 37.0 33.0 37.0 76-77 35.37155443215154 37.0 37.0 37.0 33.0 37.0 78-79 35.24420058070305 37.0 37.0 37.0 33.0 37.0 80-81 35.14509183551246 37.0 37.0 37.0 33.0 37.0 82-83 35.06139304731174 37.0 37.0 37.0 33.0 37.0 84-85 34.99395211485052 37.0 33.0 37.0 33.0 37.0 86-87 34.93236262080974 37.0 33.0 37.0 33.0 37.0 88-89 34.86448568327795 37.0 33.0 37.0 33.0 37.0 90-91 34.789931742169145 37.0 33.0 37.0 33.0 37.0 92-93 34.745569004024716 37.0 33.0 37.0 33.0 37.0 94-95 34.68391662036366 37.0 33.0 37.0 33.0 37.0 96-97 34.62631095043105 37.0 33.0 37.0 33.0 37.0 98-99 34.557005737029684 37.0 33.0 37.0 33.0 37.0 100-101 32.664599099411745 35.0 30.0 37.0 27.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.17836866731100542 2306 2 0.1831493645660629 2306 3 0.19727871369504157 2306 4 0.26720910570168144 2306 5 0.25920601380789066 2306 6 0.2855168143568889 2306 7 0.22269367030839504 2306 8 0.2520945827351966 2306 9 0.25575446495867027 2306 10-11 0.2629360832187544 2306 12-13 0.2451268821649748 2306 14-15 0.44092140996573903 2306 16-17 0.4529905628044659 2306 18-19 0.4735203362760956 2306 20-21 0.475953131279951 2306 22-23 0.4905436774345304 2306 24-25 0.5256677708024 2306 26-27 0.49837085048591234 2306 28-29 0.5205420457987557 2306 30-31 0.5115786402597493 2306 32-33 0.5230369763341969 2306 34-35 0.5426816443052118 2306 36-37 0.5751965172129445 2306 38-39 0.5587761811938137 2306 40-41 0.5274202960944407 2306 42-43 0.5649627272894762 2306 44-45 0.5871909658718124 2306 46-47 0.5884162651966705 2306 48-49 0.6440224449130625 2306 50-51 0.28191890774398587 2306 52-53 0.38578479846010794 2306 54-55 0.5711794170484623 2306 56-57 0.6276754838214131 2306 58-59 0.632711569656486 2306 60-61 0.6674472468525252 2306 62-63 0.6224843620208063 2306 64-65 0.6210115712001425 2306 66-67 0.6095908923746691 2306 68-69 0.64724315686653 2306 70-71 0.6158684647494752 2306 72-73 0.6489525305353183 2306 74-75 0.6302023703895756 2306 76-77 0.6263429760490169 2306 78-79 0.7008412528794921 2306 80-81 0.659397067225683 2306 82-83 0.6987068364979478 2306 84-85 0.6982864063890659 2306 86-87 0.7581912465974483 2306 88-89 0.7688241933756714 2306 90-91 0.7633203894060259 2306 92-93 0.8118410131262976 2306 94-95 0.7915129608390146 2306 96-97 0.7811907808209497 2306 98-99 0.7922622836414277 2306 100-101 0.6959153949065779 2307 1 0.1360393483649176 2307 2 0.1763919685719273 2307 3 0.18640271037008915 2307 4 0.20389791478709896 2307 5 0.19853658026320176 2307 6 0.2046895369674715 2307 7 0.19181249947662593 2307 8 0.1891258043938322 2307 9 0.21116432915502514 2307 10-11 0.19248598489258484 2307 12-13 0.2304223076165144 2307 14-15 0.367685718805177 2307 16-17 0.37464685313483415 2307 18-19 0.4028000204057136 2307 20-21 0.3921951696003987 2307 22-23 0.4307021215452025 2307 24-25 0.4195116683813751 2307 26-27 0.3741163546897326 2307 28-29 0.40480710170830747 2307 30-31 0.3980098243365262 2307 32-33 0.39690885152585054 2307 34-35 0.42586038197202214 2307 36-37 0.43054898097840777 2307 38-39 0.4435599451744636 2307 40-41 0.4643008477388406 2307 42-43 0.4470318700966587 2307 44-45 0.44702061431171103 2307 46-47 0.466780005479869 2307 48-49 0.4322660732858097 2307 50-51 0.3907699432679905 2307 52-53 0.44440314937296677 2307 54-55 0.4524929424755939 2307 56-57 0.4300425542756656 2307 58-59 0.43330948673365555 2307 60-61 0.4609052125773445 2307 62-63 0.4868125143630806 2307 64-65 0.44103287085420106 2307 66-67 0.4313106673532374 2307 68-69 0.453237839945686 2307 70-71 0.5102851432692219 2307 72-73 0.5077361503216551 2307 74-75 0.49167165830453285 2307 76-77 0.5234636405956152 2307 78-79 0.5553969614738676 2307 80-81 0.5253633910013065 2307 82-83 0.5575742325312305 2307 84-85 0.5333205693508276 2307 86-87 0.591648537023076 2307 88-89 0.5944459416632881 2307 90-91 0.614085206514126 2307 92-93 0.5640980456985503 2307 94-95 0.5780175959360392 2307 96-97 0.5801923493419565 2307 98-99 0.5664296266947204 2307 100-101 0.6214208090444018 2201 1 0.195165866776442 2201 2 0.2043114313225729 2201 3 0.23303963573094677 2201 4 0.28404521700490903 2201 5 0.3045332400086238 2201 6 0.31079152005555244 2201 7 0.260266402960319 2201 8 0.2791308699450781 2201 9 0.27560089942575416 2201 10-11 0.2684648650546819 2201 12-13 0.2689762933527149 2201 14-15 0.4874791095146591 2201 16-17 0.4769321189007414 2201 18-19 0.49382760007530635 2201 20-21 0.5161789247286492 2201 22-23 0.5304057115665657 2201 24-25 0.5183958678738136 2201 26-27 0.5337075496582884 2201 28-29 0.5417581664771447 2201 30-31 0.5428833360173897 2201 32-33 0.5566536430357871 2201 34-35 0.516988269587003 2201 36-37 0.5683652017762597 2201 38-39 0.5871564113255445 2201 40-41 0.5883786415658889 2201 42-43 0.6165284746727053 2201 44-45 0.6173209363002172 2201 46-47 0.5889939522965904 2201 48-49 0.6115101024553624 2201 50-51 0.5733034461217486 2201 52-53 0.6251969362988348 2201 54-55 0.6579298838857213 2201 56-57 0.6478900962294674 2201 58-59 0.6441216801675012 2201 60-61 0.6413372639751316 2201 62-63 0.6240713991393463 2201 64-65 0.621367122005239 2201 66-67 0.625399018591537 2201 68-69 0.6488627835224676 2201 70-71 0.660768376796149 2201 72-73 0.684693158336259 2201 74-75 0.6961769896718266 2201 76-77 0.6975311528582537 2201 78-79 0.7054206592259717 2201 80-81 0.7199106510897764 2201 82-83 0.7270934063949923 2201 84-85 0.73615326572871 2201 86-87 0.7731494949639597 2201 88-89 0.7344877449995266 2201 90-91 0.7108460121875453 2201 92-93 0.7608282574486296 2201 94-95 0.7962118272261023 2201 96-97 0.8011469299042844 2201 98-99 0.8045889006658129 2201 100-101 0.7899350226802682 1101 1 0.16889252590189585 1101 2 0.1650923216072897 1101 3 0.16773536417232293 1101 4 0.2287395519612403 1101 5 0.21664145407603286 1101 6 0.24763222012370534 1101 7 0.19741841227207857 1101 8 0.2428167081556083 1101 9 0.2301690712904687 1101 10-11 0.22009995581856856 1101 12-13 0.21691618964932502 1101 14-15 0.4106796402099704 1101 16-17 0.3810969119740619 1101 18-19 0.3874417791955054 1101 20-21 0.4089178137346323 1101 22-23 0.38102715905794327 1101 24-25 0.4304038154225651 1101 26-27 0.4166351329336564 1101 28-29 0.3885688422120026 1101 30-31 0.3592675292129641 1101 32-33 0.42000226371979466 1101 34-35 0.4993214846497622 1101 36-37 0.4926681665035204 1101 38-39 0.4782896893878785 1101 40-41 0.48676184761197305 1101 42-43 0.5168905465858984 1101 44-45 0.49380157271303204 1101 46-47 0.4652636923589384 1101 48-49 0.4927643912276167 1101 50-51 0.4528970578710485 1101 52-53 0.5102043252825723 1101 54-55 0.5195140480406053 1101 56-57 0.5476986969596993 1101 58-59 0.508591324442385 1101 60-61 0.5253597576341917 1101 62-63 0.4710935274476995 1101 64-65 0.5076862312521229 1101 66-67 0.4939248460757497 1101 68-69 0.45132890075788623 1101 70-71 0.483541347636006 1101 72-73 0.47179938171073843 1101 74-75 0.5199655066524329 1101 76-77 0.5304304565841349 1101 78-79 0.5328122133013906 1101 80-81 0.5879710077282638 1101 82-83 0.5765812713651215 1101 84-85 0.5503865152187402 1101 86-87 0.5567871701156548 1101 88-89 0.6430414011998309 1101 90-91 0.6230098485572668 1101 92-93 0.6632706327434263 1101 94-95 0.6670805539086473 1101 96-97 0.6204751822866754 1101 98-99 0.645019639774965 1101 100-101 0.6469431985732115 2304 1 0.11827814196369246 2304 2 0.14761156349820226 2304 3 0.14038367666920237 2304 4 0.16332988532075632 2304 5 0.15762070210721646 2304 6 0.18925030607319826 2304 7 0.1767578335159854 2304 8 0.1873939041944226 2304 9 0.17205499079582154 2304 10-11 0.18049722589760364 2304 12-13 0.17115092286889677 2304 14-15 0.34753549202955725 2304 16-17 0.3771097083670085 2304 18-19 0.3554448172009259 2304 20-21 0.35767008063643857 2304 22-23 0.3850062410876589 2304 24-25 0.39448939721309273 2304 26-27 0.3698531963926257 2304 28-29 0.38569358071506343 2304 30-31 0.3943270844718185 2304 32-33 0.38807960077060955 2304 34-35 0.38810768368016824 2304 36-37 0.4240162055442056 2304 38-39 0.4319583665168025 2304 40-41 0.44482041578681475 2304 42-43 0.4315500383001023 2304 44-45 0.4941999316189225 2304 46-47 0.4663693871018708 2304 48-49 0.5027548238310189 2304 50-51 0.3727414627290031 2304 52-53 0.41227135596250264 2304 54-55 0.47313823499039387 2304 56-57 0.49639826285692834 2304 58-59 0.4830270533137906 2304 60-61 0.5016922318057908 2304 62-63 0.46209151758043276 2304 64-65 0.4885554095983906 2304 66-67 0.4654787537146703 2304 68-69 0.49484373200757403 2304 70-71 0.49119410468839675 2304 72-73 0.4726104238429869 2304 74-75 0.4927334116067783 2304 76-77 0.5183230143633537 2304 78-79 0.549672295481038 2304 80-81 0.5196700595564252 2304 82-83 0.5345106975355236 2304 84-85 0.4930442076912058 2304 86-87 0.5749742823326542 2304 88-89 0.550933923279814 2304 90-91 0.5936645369385971 2304 92-93 0.6268814146282011 2304 94-95 0.645100084963417 2304 96-97 0.628364312938757 2304 98-99 0.5429003989550196 2304 100-101 0.5585205516886802 2202 1 0.13749791914550258 2202 2 0.1605001338103733 2202 3 0.15637856287703045 2202 4 0.21481487307338654 2202 5 0.17182267646706606 2202 6 0.2257070050004799 2202 7 0.19260611200456879 2202 8 0.19741217150027524 2202 9 0.18635838146445138 2202 10-11 0.20857702351351293 2202 12-13 0.19445843770764526 2202 14-15 0.36683383043816065 2202 16-17 0.3521004725595489 2202 18-19 0.38129929200051293 2202 20-21 0.3277744263063198 2202 22-23 0.3345123418682121 2202 24-25 0.33207457254698625 2202 26-27 0.38774571282274906 2202 28-29 0.36327104936719223 2202 30-31 0.40243854360888776 2202 32-33 0.41803024297147573 2202 34-35 0.40965046895229307 2202 36-37 0.4082999697728198 2202 38-39 0.42106487227624 2202 40-41 0.4476749965717417 2202 42-43 0.46288832615498876 2202 44-45 0.4614340740427707 2202 46-47 0.4645350348302628 2202 48-49 0.45417008712315976 2202 50-51 0.4436929747597773 2202 52-53 0.4263437516842714 2202 54-55 0.41162430558109264 2202 56-57 0.4426756119403663 2202 58-59 0.4492925432932324 2202 60-61 0.3958331784080187 2202 62-63 0.405759636331247 2202 64-65 0.4562802728789137 2202 66-67 0.42037661873277443 2202 68-69 0.41051279911959426 2202 70-71 0.40952781897301094 2202 72-73 0.4764390541038992 2202 74-75 0.4890185978584398 2202 76-77 0.45825378351677415 2202 78-79 0.500074143887538 2202 80-81 0.5003534350870282 2202 82-83 0.555336914634978 2202 84-85 0.5366503972504333 2202 86-87 0.5181121053205544 2202 88-89 0.5316749901849747 2202 90-91 0.504001107400164 2202 92-93 0.5764325970829063 2202 94-95 0.5990841956235897 2202 96-97 0.5714810466017681 2202 98-99 0.5848752683807774 2202 100-101 0.6870530834387907 1102 1 0.13129285291140036 1102 2 0.14523148869960067 1102 3 0.1398106390427003 1102 4 0.19233736019681658 1102 5 0.178014497670695 1102 6 0.16921438237396558 1102 7 0.16420823324185818 1102 8 0.18485786258085568 1102 9 0.169992568658337 1102 10-11 0.1776908600932643 1102 12-13 0.16862130702859446 1102 14-15 0.3138600462501415 1102 16-17 0.29285799626575937 1102 18-19 0.29643183606022205 1102 20-21 0.3010071238076648 1102 22-23 0.33157317919535245 1102 24-25 0.3427696408263472 1102 26-27 0.3405149150836948 1102 28-29 0.3548952446909013 1102 30-31 0.37810917204951267 1102 32-33 0.367421144252134 1102 34-35 0.3811143174959781 1102 36-37 0.3905444549173325 1102 38-39 0.4017387499533882 1102 40-41 0.37604946553416596 1102 42-43 0.38605213684049033 1102 44-45 0.37304160015659704 1102 46-47 0.3761772150067557 1102 48-49 0.39937190621824925 1102 50-51 0.3701032689891335 1102 52-53 0.39007000711922757 1102 54-55 0.41918228161096494 1102 56-57 0.39616234677563256 1102 58-59 0.4073625920915376 1102 60-61 0.43124404314106357 1102 62-63 0.38584117183477673 1102 64-65 0.38120740396499286 1102 66-67 0.42465225922673966 1102 68-69 0.40047184548535597 1102 70-71 0.3984642450146296 1102 72-73 0.4446244004856794 1102 74-75 0.422666342900591 1102 76-77 0.4350600716099109 1102 78-79 0.43028478995915265 1102 80-81 0.44642617596264245 1102 82-83 0.490064256234767 1102 84-85 0.4979562928838206 1102 86-87 0.5243371385511182 1102 88-89 0.5323811582519156 1102 90-91 0.47353850920544716 1102 92-93 0.513018293307077 1102 94-95 0.5245978193715288 1102 96-97 0.512700491876025 1102 98-99 0.4936820491709639 1102 100-101 0.5831389165523859 2305 1 0.11694291340706542 2305 2 0.1271032914351906 2305 3 0.13087824315673657 2305 4 0.17661656607341314 2305 5 0.13802559376797063 2305 6 0.1865531146744388 2305 7 0.20003621808861993 2305 8 0.19420753635469623 2305 9 0.19694933551749472 2305 10-11 0.18382055672110909 2305 12-13 0.20253018465088957 2305 14-15 0.3368045528236152 2305 16-17 0.33743382171471126 2305 18-19 0.32174322275604794 2305 20-21 0.26155764222636435 2305 22-23 0.2890010766974527 2305 24-25 0.343172698669143 2305 26-27 0.3926542897497498 2305 28-29 0.37200875778017206 2305 30-31 0.37184207761195154 2305 32-33 0.40660746693132666 2305 34-35 0.42269456304222075 2305 36-37 0.4083503115890892 2305 38-39 0.38628143177685814 2305 40-41 0.41418777911008675 2305 42-43 0.41418865077635303 2305 44-45 0.39870747813329643 2305 46-47 0.40392339986024695 2305 48-49 0.4111870023261446 2305 50-51 0.38398340685522925 2305 52-53 0.3739381350945621 2305 54-55 0.39936047815087505 2305 56-57 0.42265163699153163 2305 58-59 0.4442892075232976 2305 60-61 0.4447241644456881 2305 62-63 0.4592945153032062 2305 64-65 0.41431885527688195 2305 66-67 0.4509643214157961 2305 68-69 0.43412033541168427 2305 70-71 0.440745359124989 2305 72-73 0.4382211881598792 2305 74-75 0.43243263698798273 2305 76-77 0.46094808054690617 2305 78-79 0.4864021018788591 2305 80-81 0.4803266929969112 2305 82-83 0.45254846593738307 2305 84-85 0.4874640367258323 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-1.088808689441123 1213 62-63 -1.0694756928803173 1213 64-65 -0.9544846218249603 1213 66-67 -1.1025922458472408 1213 68-69 -1.129291465483604 1213 70-71 -1.1325928420902187 1213 72-73 -1.1712164263579439 1213 74-75 -1.17942200418927 1213 76-77 -1.1532055952208609 1213 78-79 -1.1997168939298533 1213 80-81 -1.255625830167972 1213 82-83 -1.2105483587182349 1213 84-85 -1.1474302096787241 1213 86-87 -1.2340188159775494 1213 88-89 -1.3041288761029008 1213 90-91 -1.513601829616718 1213 92-93 -1.3521089005608289 1213 94-95 -1.256071038903734 1213 96-97 -1.3827496318729615 1213 98-99 -1.3991417877534218 1213 100-101 -1.2558823099458003 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 12117.0 3 994.0 4 640.0 5 512.0 6 625.0 7 1962.0 8 3389.0 9 5661.0 10 11714.0 11 18884.0 12 17004.0 13 14505.0 14 13575.0 15 13150.0 16 13601.0 17 15023.0 18 16351.0 19 18479.0 20 20163.0 21 22576.0 22 25751.0 23 28964.0 24 33462.0 25 37918.0 26 43442.0 27 51659.0 28 63118.0 29 77980.0 30 98187.0 31 128426.0 32 174110.0 33 240321.0 34 342466.0 35 521754.0 36 1109390.0 37 5224400.0 38 5075235.0 39 2335.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 24.087777969305407 14.679121665503162 20.807100064351307 40.42600030084012 2 25.947761138678118 24.81664849469304 29.927215682784098 19.308374683844747 3 24.13198491593332 26.456014954417817 29.811250391338568 19.6007497383103 4 25.623836510629317 29.901731531056143 22.010524467647866 22.463907490666678 5 28.3422043005369 32.96135278707995 21.73701096285246 16.95943194953069 6 19.867075224043667 37.36764831631756 22.170220160136555 20.59505629950221 7 18.75865627793417 16.42148811999901 42.78907342348111 22.030782178585703 8 22.547431901567776 21.904623964736004 28.479068083117916 27.068876050578304 9 24.7794793596923 22.757512030707833 27.023352385430616 25.439656224169255 10-11 24.51313526845046 31.083160501178895 22.075631014681303 22.32807321568934 12-13 23.10415904365018 25.07430981347753 27.909697514998577 23.911833627873715 14-15 23.30303004675945 26.606914689375177 27.916631058505985 22.173424205359385 16-17 24.272473866122454 26.52186997058964 26.978272298121333 22.227383865166576 18-19 24.051690326296583 26.799435424110335 26.87514470669673 22.27372954289635 20-21 24.017887674121496 27.19029541288107 26.67007924220045 22.121737670796982 22-23 24.26090636888617 26.921938708892462 26.74722611296746 22.06992880925391 24-25 23.63053563121131 27.45392882548708 26.807380794630998 22.108154748670618 26-27 24.04249513652356 27.60552732294073 26.39353115445484 21.958446386080865 28-29 24.17403147448284 26.93658367082417 26.764371353081096 22.125013501611885 30-31 23.809367029998977 27.079736011246265 26.89035799778225 22.22053896097251 32-33 23.849268323921855 27.262259830805135 26.799541657447275 22.08893018782574 34-35 24.210933565633873 26.950880029651476 26.766822970590958 22.071363434123693 36-37 23.69361323426718 27.048479833605537 27.05017472390441 22.207732208222875 38-39 24.12360613953214 27.13116776928844 26.815554047134587 21.92967204404484 40-41 24.500553674851737 26.721652767661947 26.63133743269385 22.146456124792465 42-43 24.196061774580933 26.86493144921848 26.8675909937954 22.071415782405186 44-45 24.613481965050667 26.974013914178258 26.5454685573995 21.867035563371573 46-47 24.557424369121062 26.906038844593922 26.497410505031343 22.039126281253672 48-49 24.317407675128976 26.854004834418493 26.586412263256815 22.24217522719571 50-51 24.790077058426284 27.104225395776364 26.16230377034534 21.943393775452012 52-53 24.829539565573466 27.022772248038933 26.396917841428436 21.750770344959168 54-55 24.536599232773717 27.138741612598967 26.399156475994417 21.925502678632906 56-57 24.942966396916656 27.15325973647663 26.49157984813803 21.412194018468682 58-59 25.33016937699422 27.312238692540543 26.08057758357426 21.277014346890976 60-61 24.949431540510012 27.48660888756063 25.929023952348924 21.63493561958043 62-63 25.69279065294469 27.55033000694166 25.605652863868855 21.151226476244794 64-65 25.808010399547005 27.492111552183484 25.70545140068361 20.9944266475859 66-67 25.790073545223862 27.669633045074814 25.681075637829444 20.85921777187188 68-69 26.32696945666237 27.68972874406273 25.393877242354467 20.58942455692043 70-71 26.524784323361512 27.706847192472324 25.240370837844782 20.52799764632138 72-73 26.583514210767344 27.684380049639213 25.16060967015549 20.571496069437952 74-75 27.01064807617221 27.745935430925318 24.959879458876333 20.283537034026136 76-77 27.376316225766562 27.603801617750566 24.98599454947672 20.033887607006147 78-79 27.455251389825463 27.533235819418945 24.999902649263987 20.011610141491598 80-81 27.935500531532792 27.58349633326179 24.5638753791074 19.917127756098015 82-83 28.264042182108227 27.481188485630547 24.557534806001264 19.69723452625996 84-85 28.29312375878608 27.373482062652492 24.66699288014428 19.666401298417146 86-87 28.62942501579861 27.432827764147177 24.466960219459715 19.470787000594495 88-89 28.93678492206826 27.291025897224312 24.421334275315324 19.350854905392108 90-91 28.971757313873535 27.264273831715872 24.500451772899872 19.26351708151072 92-93 29.399152245302616 27.39037524999449 24.124438061618577 19.086034443084312 94-95 29.48352287700037 27.40040081688066 24.258909803150033 18.857166502968937 96-97 29.343890880935746 27.642209169542515 24.291830493600564 18.72206945592118 98-99 29.70504243538297 27.775565828278282 24.07147585558975 18.447915880749 100-101 29.806770017212486 27.845579430254062 23.938101483786827 18.40954906874662 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 10272.0 1 5638.0 2 782.5 3 513.5 4 434.5 5 387.5 6 349.0 7 313.5 8 321.0 9 345.0 10 349.0 11 380.0 12 425.0 13 459.5 14 518.0 15 539.0 16 574.0 17 662.5 18 775.0 19 930.0 20 1110.0 21 1331.0 22 1676.5 23 2268.5 24 3194.0 25 4486.5 26 6606.5 27 9808.0 28 14774.5 29 21460.0 30 30214.0 31 41442.5 32 54517.0 33 70331.5 34 89138.0 35 112154.0 36 144539.0 37 185100.0 38 232767.0 39 292175.0 40 366189.0 41 452353.0 42 546114.0 43 644308.0 44 739470.5 45 822752.0 46 885413.5 47 919699.0 48 923766.0 49 898352.5 50 841037.5 51 762094.5 52 667549.0 53 566471.5 54 465975.5 55 376023.0 56 304895.0 57 247727.5 58 200764.5 59 159280.0 60 123028.5 61 86973.5 62 54894.5 63 34822.0 64 22663.5 65 14232.5 66 9432.5 67 5962.5 68 4208.0 69 3450.0 70 3111.5 71 2435.0 72 1418.5 73 969.5 74 885.0 75 731.5 76 535.5 77 282.5 78 135.5 79 81.5 80 73.0 81 63.5 82 57.5 83 46.5 84 35.0 85 32.5 86 25.0 87 18.5 88 18.5 89 17.5 90 11.5 91 8.0 92 8.0 93 4.5 94 2.5 95 2.0 96 1.5 97 1.0 98 0.5 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1303644790535712 2 0.12994965941455763 3 0.12794222866147406 4 0.13560157699611766 5 0.13499416252470492 6 0.1183421170157312 7 0.11949768601012618 8 0.11646061365306248 9 0.12324587774835605 10-11 0.08744546140277334 12-13 0.0942825779529436 14-15 0.14657207494931607 16-17 0.1486646918782685 18-19 0.1480757961407403 20-21 0.17922430653452784 22-23 0.15232028994707567 24-25 0.14890543541876747 26-27 0.1369534445696887 28-29 0.14793505376321783 30-31 0.10457158649919114 32-33 0.11882730784350604 34-35 0.13246450347607747 36-37 0.1344052667871767 38-39 0.13696455581001943 40-41 0.16755009669371712 42-43 0.14882765673645243 44-45 0.13214598125326346 46-47 0.1245088553992813 48-49 0.14692763463989914 50-51 0.12224216237181425 52-53 0.12174586030370871 54-55 0.10750125019972454 56-57 0.10159007034378104 58-59 0.1129568692021085 60-61 0.11224574982094238 62-63 0.09746039268752978 64-65 0.11414577191749564 66-67 0.10565308055804797 68-69 0.11271612566160956 70-71 0.09533444203758518 72-73 0.10171229398741896 74-75 0.13312006665558998 76-77 0.16336856658258916 78-79 0.13084966988134603 80-81 0.1199791730911241 82-83 0.11025313405496641 84-85 0.10969386829165348 86-87 0.11313094530062313 88-89 0.12012361921542346 90-91 0.13712752066820333 92-93 0.11145314801068427 94-95 0.11208648870953536 96-97 0.10985312940306045 98-99 0.10185674011171833 100-101 0.09504925353576334 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.3499843E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.03065413218064 #Duplication Level Percentage of deduplicated Percentage of total 1 71.45835940500791 31.463583078149604 2 14.234317098341865 12.534925859297516 3 4.80225388925495 6.343391401581119 4 2.691564559662162 4.740453928036789 5 1.7219964104459176 3.791031418260038 6 0.96318593050355 2.5445823942590633 7 0.7817376121327921 2.409429289535502 8 0.47720350109225873 1.6809265845807144 9 0.39310899916600506 1.557796174067345 >10 2.2082491500450185 18.065204348842258 >50 0.1661323700197333 5.077282394706692 >100 0.09663572846718733 7.674700912306919 >500 0.004092899852992911 1.222238935140189 >1k 0.0011455856990151146 0.8216787087984755 >5k 1.6860308717097545E-5 0.07277457243771313 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 3.0370723570637083E-4 0.0 0.0 2 5.481545229822302E-4 0.0 0.0 3 9.407516813343681E-4 0.0 0.0 4 0.0020740981950678984 0.0 0.0 5 0.0022444705468056183 0.0 0.0 6 0.002674105172926826 0.0 0.0 7 0.003133369773263289 0.0 0.0 8 0.004466718612949795 0.0 0.0 9 0.004896353239071003 0.0 0.0 10-11 0.005818586186520836 1.1111240330720883E-5 0.0 12-13 0.006777856601739738 1.4814987107627844E-5 0.0 14-15 0.008074167973657176 2.2222480661441765E-5 0.0 16-17 0.009537147950535424 2.2222480661441765E-5 0.0 18-19 0.011989028316847833 2.2222480661441765E-5 3.703746776906961E-6 20-21 0.014840913335066193 2.2222480661441765E-5 7.407493553813922E-6 22-23 0.020585424586048888 2.9629974215255688E-5 7.407493553813922E-6 24-25 0.028837372404997598 3.703746776906961E-5 7.407493553813922E-6 26-27 0.044615333674621255 3.703746776906961E-5 7.407493553813922E-6 28-29 0.08245651449427968 4.0741214545976574E-5 7.407493553813922E-6 30-31 0.15182769162574705 5.555620165360441E-5 7.407493553813922E-6 32-33 0.25933634931902544 5.9259948430511376E-5 7.407493553813922E-6 34-35 0.4007861424758792 5.9259948430511376E-5 7.407493553813922E-6 36-37 0.5960810062754063 7.407493553813922E-5 7.407493553813922E-6 38-39 0.8445320438171022 8.148242909195315E-5 1.4814987107627844E-5 40-41 1.1554875119658798 9.629741619958099E-5 1.4814987107627844E-5 42-43 1.5422364541572817 9.629741619958099E-5 1.4814987107627844E-5 44-45 1.9895749898721045 9.629741619958099E-5 1.4814987107627844E-5 46-47 2.495554948305695 1.0000116297648794E-4 1.4814987107627844E-5 48-49 3.078524690990851 1.0370490975339491E-4 1.4814987107627844E-5 50-51 3.7193691808119547 1.0370490975339491E-4 1.4814987107627844E-5 52-53 4.422862547364439 1.1111240330720883E-4 2.2222480661441765E-5 54-55 5.189767762484349 1.1851989686102275E-4 2.2222480661441765E-5 56-57 6.021251506406408 1.222236436379297E-4 2.2222480661441765E-5 58-59 6.922424949682748 1.4074237752246453E-4 2.2222480661441765E-5 60-61 7.898073333149133 1.4814987107627844E-4 2.2222480661441765E-5 62-63 8.968263556842846 1.4814987107627844E-4 2.2222480661441765E-5 64-65 10.125502941034203 1.4814987107627844E-4 2.9629974215255688E-5 66-67 11.354809830010616 1.4814987107627844E-4 2.9629974215255688E-5 68-69 12.66329171383697 1.5185361785318541E-4 2.9629974215255688E-5 70-71 14.053359731665028 1.5555736463009236E-4 2.9629974215255688E-5 72-73 15.525047217215786 1.5555736463009236E-4 2.9629974215255688E-5 74-75 17.066016989975367 1.5555736463009236E-4 4.444496132288353E-5 76-77 18.655913257657886 1.5555736463009236E-4 4.444496132288353E-5 78-79 20.286021104097284 1.5555736463009236E-4 4.444496132288353E-5 80-81 21.95023675460522 1.5555736463009236E-4 4.444496132288353E-5 82-83 23.636941555542535 1.5555736463009236E-4 4.444496132288353E-5 84-85 25.358687504736167 1.5555736463009236E-4 4.444496132288353E-5 86-87 27.084533501611833 1.5555736463009236E-4 4.444496132288353E-5 88-89 28.813268420973486 1.5555736463009236E-4 4.444496132288353E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGGCGC 2330 0.0 28.237782 42-43 TCGGGGG 3880 0.0 27.370178 38-39 GGCGCCC 990 0.0 27.348461 44-45 GGGGGCG 2935 0.0 23.231283 40-41 GGGGGGC 3075 0.0 22.559898 40-41 GGGCGCC 3635 0.0 21.89007 42-43 AGGGTAG 3940 0.0 19.048561 28-29 CTCGGGG 3615 0.0 18.795815 36-37 CGGGTAG 1990 0.0 18.386442 14-15 AAGGGGC 2235 0.0 18.27732 9 CGGGGGG 3715 0.0 18.225853 38-39 GGGCCCG 605 0.0 18.057837 44-45 GCGCCCG 1250 0.0 18.049984 44-45 GTAGGGG 2630 0.0 17.79869 18-19 GATCGGG 4570 0.0 17.150513 1 GGGTAGG 2310 0.0 16.766792 16-17 GAGCGGC 4020 0.0 16.660374 9 GAGGGGC 1755 0.0 16.509897 9 GGGTAGA 4815 0.0 16.3762 28-29 GGGGCCC 1125 0.0 16.04443 44-45 >>END_MODULE