FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l03n02 tsrexp_lib#5.3420000000caba.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l03n02 tsrexp_lib#5.3420000000caba.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13499843
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGCGC23300.028.23778242-43
TCGGGGG38800.027.37017838-39
GGCGCCC9900.027.34846144-45
GGGGGCG29350.023.23128340-41
GGGGGGC30750.022.55989840-41
GGGCGCC36350.021.8900742-43
AGGGTAG39400.019.04856128-29
CTCGGGG36150.018.79581536-37
CGGGTAG19900.018.38644214-15
AAGGGGC22350.018.277329
CGGGGGG37150.018.22585338-39
GGGCCCG6050.018.05783744-45
GCGCCCG12500.018.04998444-45
GTAGGGG26300.017.7986918-19
GATCGGG45700.017.1505131
GGGTAGG23100.016.76679216-17
GAGCGGC40200.016.6603749
GAGGGGC17550.016.5098979
GGGTAGA48150.016.376228-29
GGGGCCC11250.016.0444344-45
Produced by FastQC (version 0.11.2)