##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C5B16ACXX l03n02 tsrexp_lib#39.3420000000ce5a.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8835098 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.63888448096444 33.0 33.0 33.0 33.0 33.0 2 32.60312845426276 33.0 33.0 33.0 33.0 33.0 3 32.59435797995676 33.0 33.0 33.0 33.0 33.0 4 36.48904981020018 37.0 37.0 37.0 37.0 37.0 5 36.48644893356022 37.0 37.0 37.0 37.0 37.0 6 36.48376090451968 37.0 37.0 37.0 37.0 37.0 7 36.519177376413936 37.0 37.0 37.0 37.0 37.0 8 36.519434193033284 37.0 37.0 37.0 37.0 37.0 9 36.50421761026307 37.0 37.0 37.0 37.0 37.0 10-11 36.49021867103228 37.0 37.0 37.0 37.0 37.0 12-13 36.490348380968726 37.0 37.0 37.0 37.0 37.0 14-15 38.87994151281627 40.0 40.0 40.0 37.0 40.0 16-17 38.86722914675083 40.0 40.0 40.0 37.0 40.0 18-19 38.85708890835167 40.0 40.0 40.0 37.0 40.0 20-21 38.82819590682526 40.0 40.0 40.0 37.0 40.0 22-23 38.809512752433534 40.0 40.0 40.0 37.0 40.0 24-25 38.78888740113579 40.0 40.0 40.0 37.0 40.0 26-27 38.77116546981143 40.0 40.0 40.0 37.0 40.0 28-29 38.736034676695155 40.0 40.0 40.0 37.0 40.0 30-31 38.723482580498825 40.0 40.0 40.0 37.0 40.0 32-33 38.6895805230457 40.0 40.0 40.0 37.0 40.0 34-35 38.65146945738462 40.0 40.0 40.0 37.0 40.0 36-37 38.62563810837186 40.0 40.0 40.0 37.0 40.0 38-39 38.58782109717402 40.0 40.0 40.0 37.0 40.0 40-41 38.530689416235106 40.0 40.0 40.0 37.0 40.0 42-43 38.47484017721139 40.0 38.5 40.0 37.0 40.0 44-45 38.41595362043522 40.0 37.0 40.0 37.0 40.0 46-47 38.34157136683713 40.0 37.0 40.0 37.0 40.0 48-49 38.24220551939548 40.0 37.0 40.0 37.0 40.0 50-51 36.88983738493902 38.5 37.0 38.5 35.0 40.0 52-53 37.204142783702 38.5 37.0 40.0 33.0 40.0 54-55 37.85841368143285 40.0 37.0 40.0 37.0 40.0 56-57 37.79631748283947 40.0 37.0 40.0 37.0 40.0 58-59 37.618310176072754 40.0 37.0 40.0 35.0 40.0 60-61 37.394119001283286 37.0 37.0 40.0 33.0 40.0 62-63 37.164875930068916 37.0 37.0 40.0 33.0 40.0 64-65 36.90714919064848 37.0 37.0 40.0 33.0 40.0 66-67 36.63868329474104 37.0 37.0 40.0 33.0 40.0 68-69 36.36677131368548 37.0 37.0 37.0 33.0 40.0 70-71 36.115265557891945 37.0 37.0 37.0 33.0 40.0 72-73 35.893995742888194 37.0 37.0 37.0 33.0 38.5 74-75 35.68857549740818 37.0 37.0 37.0 33.0 37.0 76-77 35.51621210087313 37.0 37.0 37.0 33.0 37.0 78-79 35.38916240657433 37.0 37.0 37.0 33.0 37.0 80-81 35.29104272527594 37.0 37.0 37.0 33.0 37.0 82-83 35.20897419587196 37.0 37.0 37.0 33.0 37.0 84-85 35.144243957452424 37.0 37.0 37.0 33.0 37.0 86-87 35.08377581097572 37.0 35.0 37.0 33.0 37.0 88-89 35.01718922642397 37.0 33.0 37.0 33.0 37.0 90-91 34.946831263218584 37.0 33.0 37.0 33.0 37.0 92-93 34.903195980395466 37.0 33.0 37.0 33.0 37.0 94-95 34.84389561949398 37.0 33.0 37.0 33.0 37.0 96-97 34.788691195049566 37.0 33.0 37.0 33.0 37.0 98-99 34.72825281621098 37.0 33.0 37.0 33.0 37.0 100-101 32.87902714831233 35.0 30.0 37.0 27.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.17493262153195843 2306 2 0.20349621073690827 2306 3 0.19708356706161112 2306 4 0.29018046231472283 2306 5 0.288255997225626 2306 6 0.2485344328397474 2306 7 0.20573521367013825 2306 8 0.25123280783604685 2306 9 0.2564769783066012 2306 10-11 0.26000402226137 2306 12-13 0.27373743775560655 2306 14-15 0.5304165187936007 2306 16-17 0.5295113710590655 2306 18-19 0.528427883474123 2306 20-21 0.5081925606639714 2306 22-23 0.5210446353989653 2306 24-25 0.5486312962156958 2306 26-27 0.5373046340205718 2306 28-29 0.5481634813658971 2306 30-31 0.5272856549944507 2306 32-33 0.5418115439287305 2306 34-35 0.5905952245760631 2306 36-37 0.6065767350644293 2306 38-39 0.5600293083622603 2306 40-41 0.5710456251714646 2306 42-43 0.6382339961023789 2306 44-45 0.614187154071395 2306 46-47 0.637836891683591 2306 48-49 0.671215661908299 2306 50-51 0.3277614494396275 2306 52-53 0.4603736831513814 2306 54-55 0.6249119200458964 2306 56-57 0.6606752073592972 2306 58-59 0.6860793982249831 2306 60-61 0.6916390602773035 2306 62-63 0.6786258852880707 2306 64-65 0.6562464665812939 2306 66-67 0.6331507362660815 2306 68-69 0.6434446926465895 2306 70-71 0.6548324916853474 2306 72-73 0.6824899058294776 2306 74-75 0.6920077225474373 2306 76-77 0.6651391299801119 2306 78-79 0.6760203840567982 2306 80-81 0.711299213971536 2306 82-83 0.7740946312370767 2306 84-85 0.796449111517255 2306 86-87 0.8016845272751638 2306 88-89 0.7941452670046729 2306 90-91 0.8090806517492197 2306 92-93 0.8483354856273522 2306 94-95 0.8484436377728244 2306 96-97 0.8258467160502576 2306 98-99 0.7909283342705251 2306 100-101 0.5840794585157667 2307 1 0.154929589134845 2307 2 0.16533101632185776 2307 3 0.18122157221993263 2307 4 0.23585922682286764 2307 5 0.19315131166165855 2307 6 0.2159180669205938 2307 7 0.18087875080708926 2307 8 0.19943252795994937 2307 9 0.21487438079281418 2307 10-11 0.18341333044445207 2307 12-13 0.18852378716594131 2307 14-15 0.3640499645096895 2307 16-17 0.3851186897854646 2307 18-19 0.3982832054180676 2307 20-21 0.38501636365884906 2307 22-23 0.43099785539519786 2307 24-25 0.4268330041025834 2307 26-27 0.3936797675289796 2307 28-29 0.3977967080626428 2307 30-31 0.35567900771084027 2307 32-33 0.3855884681987334 2307 34-35 0.4273011909856024 2307 36-37 0.4595596498152119 2307 38-39 0.4649504486363796 2307 40-41 0.4780331950114345 2307 42-43 0.418559866846401 2307 44-45 0.4328164113904833 2307 46-47 0.39336772474711523 2307 48-49 0.38892649094177045 2307 50-51 0.37299864576923625 2307 52-53 0.4101135425729794 2307 54-55 0.45008577245354076 2307 56-57 0.4524165625623908 2307 58-59 0.42369661214807053 2307 60-61 0.5101173937767172 2307 62-63 0.4653735007555184 2307 64-65 0.41380306910398446 2307 66-67 0.41652885565320474 2307 68-69 0.4561005974743324 2307 70-71 0.49219737223453564 2307 72-73 0.5314667705187901 2307 74-75 0.5184110356558946 2307 76-77 0.4809116667867883 2307 78-79 0.5090495109619155 2307 80-81 0.5749324520368617 2307 82-83 0.5283726138879103 2307 84-85 0.49202349961745284 2307 86-87 0.563297787481396 2307 88-89 0.5792423344539372 2307 90-91 0.5820490152860742 2307 92-93 0.5460206755924091 2307 94-95 0.5022069365568527 2307 96-97 0.4997626224811995 2307 98-99 0.5331048599206696 2307 100-101 0.626199486338642 2201 1 0.2279203360443134 2201 2 0.21836277292856465 2201 3 0.23456932556955223 2201 4 0.28011188801170306 2201 5 0.30938499291119825 2201 6 0.29543151721298955 2201 7 0.27926853539513985 2201 8 0.30313441559041365 2201 9 0.2721107121274855 2201 10-11 0.25352695839618633 2201 12-13 0.29299430311827024 2201 14-15 0.5234832164158263 2201 16-17 0.5046621052862434 2201 18-19 0.5437296189201746 2201 20-21 0.5543247950749475 2201 22-23 0.5597662172280451 2201 24-25 0.5487288217056587 2201 26-27 0.5450697741912052 2201 28-29 0.5737155621599044 2201 30-31 0.5867342745699773 2201 32-33 0.5921496792432208 2201 34-35 0.5931707890313476 2201 36-37 0.6109775786034177 2201 38-39 0.6303194470818596 2201 40-41 0.6376317145169494 2201 42-43 0.6402191606505312 2201 44-45 0.6506241691815831 2201 46-47 0.6490703082099856 2201 48-49 0.6311413995212334 2201 50-51 0.6520837742021328 2201 52-53 0.6874224033324765 2201 54-55 0.7392284329021308 2201 56-57 0.7281046963990931 2201 58-59 0.7292156142947874 2201 60-61 0.7508571228462273 2201 62-63 0.7038606870757462 2201 64-65 0.6747546717392652 2201 66-67 0.6916726856924384 2201 68-69 0.6813989171130928 2201 70-71 0.6933349545870371 2201 72-73 0.6890534904832606 2201 74-75 0.7130525191113719 2201 76-77 0.7177640762915658 2201 78-79 0.7105724068091348 2201 80-81 0.7125940850222818 2201 82-83 0.7431222474655215 2201 84-85 0.7569848439064444 2201 86-87 0.781014850180334 2201 88-89 0.7942111404351309 2201 90-91 0.7715222405433124 2201 92-93 0.8250078249002755 2201 94-95 0.8274338519466014 2201 96-97 0.8274647321858382 2201 98-99 0.9028971465444044 2201 100-101 0.7650443897363814 1101 1 0.1930056042521926 1101 2 0.18265022090007932 1101 3 0.20114162050627726 1101 4 0.23854088020534192 1101 5 0.22667737878285266 1101 6 0.24468450686580212 1101 7 0.22113793010954907 1101 8 0.23928951312814917 1101 9 0.2715703982013977 1101 10-11 0.2322495232959696 1101 12-13 0.23761796960106096 1101 14-15 0.45692196739788926 1101 16-17 0.4244410531825409 1101 18-19 0.4495821639279427 1101 20-21 0.47007747882533835 1101 22-23 0.4471113921607639 1101 24-25 0.47031009980940297 1101 26-27 0.49649134783345517 1101 28-29 0.45568601499577 1101 30-31 0.4095873043411373 1101 32-33 0.47825487624103147 1101 34-35 0.5187293066583436 1101 36-37 0.5218395280208696 1101 38-39 0.5351138305129837 1101 40-41 0.5038861517735214 1101 42-43 0.5281109614629216 1101 44-45 0.546019251340617 1101 46-47 0.5473845619251136 1101 48-49 0.5573840419831626 1101 50-51 0.46084809561698137 1101 52-53 0.5381305122537938 1101 54-55 0.5616216529011311 1101 56-57 0.5600219857359647 1101 58-59 0.5479979154164241 1101 60-61 0.5397407294858141 1101 62-63 0.5471673617660855 1101 64-65 0.5196572276124201 1101 66-67 0.5268582163411892 1101 68-69 0.5496108088604146 1101 70-71 0.5525281210949018 1101 72-73 0.5251832733426909 1101 74-75 0.5221441795095458 1101 76-77 0.5501836741051207 1101 78-79 0.5816487219387625 1101 80-81 0.5930077720657252 1101 82-83 0.6289260085607609 1101 84-85 0.602012983585162 1101 86-87 0.6019745880737943 1101 88-89 0.6392774326638744 1101 90-91 0.6286889891182739 1101 92-93 0.636721165922971 1101 94-95 0.6646621955602541 1101 96-97 0.5915093036717209 1101 98-99 0.6592027815008592 1101 100-101 0.672854469471595 2304 1 0.15582587748753696 2304 2 0.15925693042220956 2304 3 0.18588282636925157 2304 4 0.20864559767395008 2304 5 0.19329271706209994 2304 6 0.23634381376017188 2304 7 0.2315532658495556 2304 8 0.20288107096721575 2304 9 0.20818996699623682 2304 10-11 0.21015599217170688 2304 12-13 0.2290735956199157 2304 14-15 0.3920651338116272 2304 16-17 0.39140384957075014 2304 18-19 0.443678475821109 2304 20-21 0.4146215680545282 2304 22-23 0.40575541658829195 2304 24-25 0.4246744798108395 2304 26-27 0.437529497589459 2304 28-29 0.45008993416103493 2304 30-31 0.42245413532828024 2304 32-33 0.3973776028484508 2304 34-35 0.4594691802252484 2304 36-37 0.41463090441018124 2304 38-39 0.4871019477874725 2304 40-41 0.4904148173488281 2304 42-43 0.4884182010118039 2304 44-45 0.5110117879603919 2304 46-47 0.511514827920216 2304 48-49 0.5160696900471535 2304 50-51 0.38914596255475686 2304 52-53 0.4852291208035453 2304 54-55 0.5248969623969089 2304 56-57 0.5279281981396124 2304 58-59 0.4891039198738625 2304 60-61 0.5245359261202864 2304 62-63 0.5294218499320849 2304 64-65 0.5568478884106369 2304 66-67 0.5361634185929987 2304 68-69 0.5313772782006865 2304 70-71 0.5072604790954642 2304 72-73 0.4738183789958157 2304 74-75 0.5022837920439116 2304 76-77 0.5828647254589399 2304 78-79 0.6004889746894619 2304 80-81 0.5522472979303572 2304 82-83 0.5941988492154593 2304 84-85 0.5002328634755528 2304 86-87 0.5907308347508504 2304 88-89 0.583357664096809 2304 90-91 0.5956164356698466 2304 92-93 0.6162634086805028 2304 94-95 0.6532817774334703 2304 96-97 0.6573728326656578 2304 98-99 0.6432213539016303 2304 100-101 0.6139318862082987 2202 1 0.14527495573419458 2202 2 0.1713019416596353 2202 3 0.18055218638247084 2202 4 0.22663072460221656 2202 5 0.20566274316351496 2202 6 0.24882547996186588 2202 7 0.2052164572228321 2202 8 0.2116712721827625 2202 9 0.24113708151917024 2202 10-11 0.23486102574180734 2202 12-13 0.22180160654060188 2202 14-15 0.38607549093642035 2202 16-17 0.39562164285849377 2202 18-19 0.42011629659702976 2202 20-21 0.4180477421343056 2202 22-23 0.4045974127505758 2202 24-25 0.390063659764202 2202 26-27 0.443145414158181 2202 28-29 0.4416253887495003 2202 30-31 0.45308425163209165 2202 32-33 0.46493209964876314 2202 34-35 0.4609718349988654 2202 36-37 0.44611265521595556 2202 38-39 0.4464169973158292 2202 40-41 0.43552506253052314 2202 42-43 0.4757449520075383 2202 44-45 0.44905613541701683 2202 46-47 0.47422278091390524 2202 48-49 0.44919505581997754 2202 50-51 0.4484783058105464 2202 52-53 0.4466366064703706 2202 54-55 0.44645155428941763 2202 56-57 0.47922351463930113 2202 58-59 0.43000621893741453 2202 60-61 0.45443928634746555 2202 62-63 0.4962441717991197 2202 64-65 0.5284717834008816 2202 66-67 0.479616717254963 2202 68-69 0.46227514428989736 2202 70-71 0.4955128057660474 2202 72-73 0.5294146245322509 2202 74-75 0.5634181673359748 2202 76-77 0.5124680674834181 2202 78-79 0.5413586440786062 2202 80-81 0.5460461448442828 2202 82-83 0.5714559661553125 2202 84-85 0.5927128189500266 2202 86-87 0.5525330812107043 2202 88-89 0.6065728616939623 2202 90-91 0.5667542067787465 2202 92-93 0.5681387176427322 2202 94-95 0.5868132078508026 2202 96-97 0.6213228109086586 2202 98-99 0.6618370492877759 2202 100-101 0.8285044502923924 1102 1 0.12055790149312884 1102 2 0.13260107892526207 1102 3 0.12580260394034326 1102 4 0.15174291002564644 1102 5 0.14688180219118863 1102 6 0.14374662034006747 1102 7 0.1460196245212373 1102 8 0.1360048833505445 1102 9 0.12426699087433946 1102 10-11 0.13187490019375048 1102 12-13 0.1473532605657013 1102 14-15 0.2558517894147485 1102 16-17 0.23024016655501356 1102 18-19 0.27490091111838666 1102 20-21 0.3005737001633122 1102 22-23 0.2679999295136213 1102 24-25 0.251762765412451 1102 26-27 0.27107539772674727 1102 28-29 0.297158103164854 1102 30-31 0.3459193941858203 1102 32-33 0.34348334813731896 1102 34-35 0.30622877740242416 1102 36-37 0.33565180117278004 1102 38-39 0.35793156198248965 1102 40-41 0.3543535635724666 1102 42-43 0.32197141266823337 1102 44-45 0.2614795598495263 1102 46-47 0.3120235553389179 1102 48-49 0.31496681535622884 1102 50-51 0.30656391536907535 1102 52-53 0.30702157021610077 1102 54-55 0.328668648123859 1102 56-57 0.3184736396719714 1102 58-59 0.34114713675710817 1102 60-61 0.3659299337461661 1102 62-63 0.31836777724323895 1102 64-65 0.34087277755661916 1102 66-67 0.3150986453672928 1102 68-69 0.30966036523134477 1102 70-71 0.3285904841077141 1102 72-73 0.3805914580264371 1102 74-75 0.4170098566102851 1102 76-77 0.4125161026735853 1102 78-79 0.37454953082198017 1102 80-81 0.4141131917363836 1102 82-83 0.4294494539221745 1102 84-85 0.4510007309728863 1102 86-87 0.4572667868866347 1102 88-89 0.4627989618936681 1102 90-91 0.43146436209730155 1102 92-93 0.4690826202371383 1102 94-95 0.45730378165240637 1102 96-97 0.42412344953375936 1102 98-99 0.49439721886353283 1102 100-101 0.503904269975898 2305 1 0.12907804872696715 2305 2 0.11533674849746234 2305 3 0.133213723712025 2305 4 0.17987067108697374 2305 5 0.13829884128585945 2305 6 0.1953402097270498 2305 7 0.15940201580531976 2305 8 0.20098274667674332 2305 9 0.17014573275909584 2305 10-11 0.18285026871471644 2305 12-13 0.21519358135225986 2305 14-15 0.35550784906416055 2305 16-17 0.33908478780453066 2305 18-19 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70-71 -1.1082973782530914 1213 72-73 -1.167890284617144 1213 74-75 -1.203103938297211 1213 76-77 -1.1930180627910758 1213 78-79 -1.2271966060845472 1213 80-81 -1.2764527690607181 1213 82-83 -1.2333399592560994 1213 84-85 -1.1963097802040394 1213 86-87 -1.2913096830402608 1213 88-89 -1.342358626351313 1213 90-91 -1.4895543535523714 1213 92-93 -1.3477449264560235 1213 94-95 -1.2803903078504888 1213 96-97 -1.4038458152671254 1213 98-99 -1.464429916092108 1213 100-101 -1.2929628391792818 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7547.0 3 674.0 4 470.0 5 380.0 6 337.0 7 804.0 8 1440.0 9 2401.0 10 4080.0 11 5808.0 12 6195.0 13 6247.0 14 6370.0 15 6839.0 16 7435.0 17 8504.0 18 9700.0 19 11195.0 20 12454.0 21 14219.0 22 16220.0 23 18316.0 24 21273.0 25 24256.0 26 28482.0 27 33692.0 28 41196.0 29 50647.0 30 64226.0 31 83364.0 32 111995.0 33 153467.0 34 218951.0 35 334776.0 36 699715.0 37 3308647.0 38 3511497.0 39 1279.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 24.253068082412085 14.923067176926738 20.703758172175263 40.120106568485916 2 26.397161117656363 25.028830344753807 30.497813018895208 18.07619551869462 3 23.11842378282648 27.801233795204773 29.808890591759113 19.271451830209635 4 25.130170289553668 31.622727275818214 21.3561555683145 21.890946866313623 5 27.205381191533363 34.36889068910703 21.293817890204274 17.131910229155327 6 18.711643337415342 38.00591077452314 22.26162083857938 21.02082504948214 7 17.88552171917198 15.722528182622419 43.87489513989638 22.51705495830922 8 22.11277131959285 21.199225433291524 28.19060895089361 28.497394296222023 9 24.37886379300302 22.911310871914885 26.77581212602926 25.934013209052832 10-11 24.168596810169884 31.454828355004576 21.84901488907969 22.52755994574585 12-13 22.700293357455976 25.50307206828052 28.225626379626867 23.57100819463664 14-15 23.13744446010538 26.910498981878405 27.737696920483256 22.214359637532958 16-17 23.898591266398018 27.282250790876038 26.755226798277388 22.063931144448553 18-19 23.731041086395376 27.561246752157416 26.79366632085355 21.91404584059366 20-21 23.93213329110883 27.649896712101096 26.609018424239046 21.808951572551035 22-23 23.901106141461305 27.62192763279266 26.74000135458617 21.73696487115987 24-25 23.46743274933113 28.051902255429866 26.684879058207546 21.795785937031457 26-27 23.84334116832632 28.08321367770128 26.443690677242376 21.629754476730024 28-29 23.887745037669635 27.47090140923138 26.832155795350904 21.809197757748088 30-31 23.467592237743013 27.720480979266256 26.86643058327395 21.945496199716782 32-33 23.83089482154046 27.79407116216764 26.5782383779015 21.7967956383904 34-35 23.8429517512015 27.608895867088417 26.71610414282809 21.832048238881992 36-37 23.54589491118611 27.739316510059385 26.867106759022064 21.847681819732436 38-39 23.969365600545878 27.72112580006944 26.54125589205345 21.768252707331232 40-41 24.12130990976038 27.494377879933555 26.655362339346134 21.72894987095993 42-43 23.835691219136503 27.521846047784248 26.848601871644767 21.79386086143448 44-45 24.169214745841852 27.6148958933048 26.524427560507906 21.691461800345436 46-47 24.135384782754045 27.501011408231555 26.644867089059453 21.718736719954943 48-49 23.878442887414984 27.545785677406997 26.832909126871453 21.742862308306567 50-51 24.38116831578151 27.685825151974065 26.443056696944385 21.48994983530005 52-53 24.456720448186825 27.468469334697392 26.53508673769481 21.539723479420964 54-55 24.256845007020555 27.585316684167303 26.525840094472297 21.631998214339838 56-57 24.5498571707522 27.67416022423968 26.332641039031724 21.443341565976397 58-59 24.757274017853064 27.580245713352703 26.32225847706761 21.34022179172662 60-61 24.523556940686777 27.73146268856274 26.347163652051687 21.397816718698788 62-63 24.93683877987041 27.79935438784753 25.954872858181655 21.308933974100402 64-65 25.058856402841243 27.705261457041708 26.078001036339533 21.157881103777516 66-67 24.852846245336607 27.870926515037997 26.087757755940295 21.1884694836851 68-69 25.38951205403908 27.798988495223764 25.778262108568782 21.033237342168373 70-71 25.546134625484708 27.733125819357713 25.740214495556607 20.98052505960098 72-73 25.444773795317328 27.849692282958966 25.81978853793251 20.885745383791203 74-75 25.952331342440694 27.942415544500477 25.46458805091552 20.64066506214331 76-77 26.14578943913718 27.738201438465595 25.525423801829444 20.59058532056778 78-79 26.102621685833792 27.88904266512296 25.44460497011034 20.563730678932902 80-81 26.66354967350894 27.90282641626771 25.02661833589734 20.40700557432601 82-83 26.855469779049677 27.780954125866554 25.132474676933107 20.231101418150665 84-85 26.822201534043614 27.905196147708306 25.14487846626684 20.127723851981237 86-87 27.225940639618905 28.018117551971933 24.75476791921739 20.001173889191772 88-89 27.559139348123928 27.90959201638985 24.806732036375735 19.724536599110486 90-91 27.53252296467041 28.03947467292862 24.766919178209967 19.661083184191 92-93 27.96896629594659 28.095923182047528 24.493274384033334 19.441836137972544 94-95 28.30065183218814 27.994921721103477 24.47809631053713 19.226330136171256 96-97 28.21149718425216 28.162123941173384 24.435149420769427 19.19122945380503 98-99 28.69137877892579 28.185062529170303 24.224348305971386 18.899210385932523 100-101 28.888645569517717 28.124342039339457 24.116917311738355 18.87009507940447 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 6362.0 1 3493.0 2 502.5 3 332.5 4 270.5 5 237.5 6 236.5 7 235.5 8 211.5 9 213.5 10 236.0 11 229.5 12 259.0 13 308.0 14 331.0 15 364.5 16 400.0 17 448.5 18 522.5 19 628.5 20 769.5 21 951.5 22 1230.0 23 1652.0 24 2364.5 25 3504.5 26 5213.5 27 7679.0 28 11245.0 29 16206.0 30 21689.5 31 28424.5 32 37038.0 33 47141.5 34 59480.0 35 76413.5 36 101472.0 37 132217.5 38 169194.5 39 215346.0 40 271143.0 41 333186.5 42 401251.5 43 469564.0 44 530383.0 45 579754.5 46 613389.5 47 628843.5 48 619726.5 49 590846.5 50 543464.0 51 478774.0 52 406128.5 53 337498.5 54 269594.5 55 206507.5 56 157241.0 57 118383.5 58 88825.0 59 65232.5 60 46837.0 61 32661.5 62 23400.5 63 17205.5 64 12336.0 65 9579.0 66 7455.0 67 5359.5 68 4429.5 69 3823.5 70 3209.5 71 2219.5 72 1161.5 73 850.0 74 892.5 75 798.0 76 603.5 77 252.0 78 75.0 79 54.5 80 47.5 81 41.5 82 40.5 83 30.5 84 21.0 85 17.0 86 16.5 87 14.5 88 11.5 89 9.5 90 7.0 91 7.0 92 6.0 93 3.0 94 2.0 95 2.0 96 1.5 97 2.0 98 1.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1256465972420453 2 0.12520517599238853 3 0.12202467929614362 4 0.13192836117946855 5 0.12989103233489882 6 0.11541467904487307 7 0.11595796673675833 8 0.11358108308476035 9 0.1193421963174602 10-11 0.08360405283563352 12-13 0.09035553425666586 14-15 0.14401085307712488 16-17 0.1462236185721992 18-19 0.14595763397304706 20-21 0.1762855375231831 22-23 0.14957955191894873 24-25 0.1460029079473708 26-27 0.1342486523635618 28-29 0.14495028804434312 30-31 0.10163441311007529 32-33 0.11638241024604368 34-35 0.1293420853962231 36-37 0.13182649473724004 38-39 0.13445804449480922 40-41 0.16504627339730696 42-43 0.14673861003013208 44-45 0.12968729945044186 46-47 0.12187753887959137 48-49 0.14423156370195328 50-51 0.11959120317624095 52-53 0.11855556101358468 54-55 0.10447535499889192 56-57 0.09789931022836419 58-59 0.10878204180644062 60-61 0.10888956749545958 62-63 0.09373410459057727 64-65 0.11097782956114352 66-67 0.1023248412185128 68-69 0.10903104866522137 70-71 0.09124969524956032 72-73 0.09784837700724995 74-75 0.13017965392121286 76-77 0.1603943725355395 78-79 0.12801216240046234 80-81 0.11719168253708108 82-83 0.10694844584632791 84-85 0.10569775230563373 86-87 0.1101685572701061 88-89 0.11735580069400477 90-91 0.1343618372993712 92-93 0.10934796648548777 94-95 0.10941587744697343 96-97 0.10715783797757535 98-99 0.09844259792024944 100-101 0.09176468670749323 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 8835098.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.78336651536606 #Duplication Level Percentage of deduplicated Percentage of total 1 70.47742332999228 34.38125973365624 2 15.18003229010494 14.810661578465616 3 5.469341108376616 8.004386156624847 4 2.6341169662103248 5.140043736279296 5 1.4004110240343526 3.4158382128813463 6 1.1548048001453797 3.3801159491517665 7 0.6480536400160544 2.2129966769764198 8 0.5035633650998649 1.9652412962702241 9 0.4166389397499839 1.8292545092157282 >10 1.9609029239419546 17.064044235708334 >50 0.10861154438673488 3.569062409987416 >100 0.04462104344836674 3.6787842689377404 >500 0.00131621551389546 0.43524004337014294 >1k 1.6280897904787984E-4 0.11307119247481896 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 1.8109589729508376E-4 0.0 1.1318493580942735E-5 2 2.263698716188547E-4 0.0 1.1318493580942735E-5 3 2.4900685878074017E-4 0.0 1.1318493580942735E-5 4 3.508733010092248E-4 0.0 1.1318493580942735E-5 5 3.84828781752053E-4 0.0 1.1318493580942735E-5 6 4.0746576891393846E-4 0.0 1.1318493580942735E-5 7 4.187842624948812E-4 0.0 1.1318493580942735E-5 8 4.753767303995949E-4 0.0 1.1318493580942735E-5 9 4.753767303995949E-4 0.0 1.1318493580942735E-5 10-11 5.093322111424231E-4 5.6592467904713676E-6 1.1318493580942735E-5 12-13 5.99880159789965E-4 2.263698716188547E-5 1.1318493580942735E-5 14-15 6.960873552279783E-4 2.8296233952356838E-5 1.1318493580942735E-5 16-17 8.319092781992911E-4 3.3955480742828205E-5 1.1318493580942735E-5 18-19 0.001029982915865789 5.093322111424231E-5 1.1318493580942735E-5 20-21 0.0020090326106173353 5.6592467904713676E-5 1.1318493580942735E-5 22-23 0.007164606436736751 5.6592467904713676E-5 1.1318493580942735E-5 24-25 0.013502962842064682 5.6592467904713676E-5 1.1318493580942735E-5 26-27 0.025291173906616542 5.6592467904713676E-5 1.1318493580942735E-5 28-29 0.0525574249431076 5.6592467904713676E-5 1.1318493580942735E-5 30-31 0.09853880511568745 5.6592467904713676E-5 1.1318493580942735E-5 32-33 0.16273730070679465 6.791096148565641E-5 1.1318493580942735E-5 34-35 0.22969750873165187 9.054794864754188E-5 1.1318493580942735E-5 36-37 0.31559355651742627 9.054794864754188E-5 1.1318493580942735E-5 38-39 0.42611864633533214 9.054794864754188E-5 1.1318493580942735E-5 40-41 0.5670904838859738 1.0752568901895598E-4 1.1318493580942735E-5 42-43 0.7457076310868311 1.1318493580942735E-4 1.1318493580942735E-5 44-45 0.9469561062027835 1.1318493580942735E-4 1.1318493580942735E-5 46-47 1.1741861833337899 1.1884418259989872E-4 1.1318493580942735E-5 48-49 1.4370242412704421 1.2450342939037009E-4 2.263698716188547E-5 50-51 1.7465850407092258 1.3016267618084145E-4 2.263698716188547E-5 52-53 2.1101010990483635 1.3582192297131282E-4 2.263698716188547E-5 54-55 2.527408298130932 1.4714041655225556E-4 2.263698716188547E-5 56-57 2.998234994110988 1.4714041655225556E-4 2.263698716188547E-5 58-59 3.5337016069318077 1.584589101331983E-4 3.3955480742828205E-5 60-61 4.145341681552372 1.584589101331983E-4 3.3955480742828205E-5 62-63 4.838022170212486 1.6977740371414103E-4 3.3955480742828205E-5 64-65 5.6072383124669365 1.8109589729508376E-4 3.3955480742828205E-5 66-67 6.441869688372444 1.8675514408555513E-4 3.3955480742828205E-5 68-69 7.351587950693926 2.0373288445696923E-4 3.3955480742828205E-5 70-71 8.329602003282815 2.0373288445696923E-4 3.3955480742828205E-5 72-73 9.382369046727042 2.0373288445696923E-4 3.3955480742828205E-5 74-75 10.51211882426205 2.1505137803791197E-4 3.3955480742828205E-5 76-77 11.70189623250359 2.3768836519979744E-4 3.3955480742828205E-5 78-79 12.957388814476081 2.433476119902688E-4 3.3955480742828205E-5 80-81 14.282795731298057 2.4900685878074017E-4 3.3955480742828205E-5 82-83 15.67302366085809 2.4900685878074017E-4 3.3955480742828205E-5 84-85 17.126069229792357 2.4900685878074017E-4 3.3955480742828205E-5 86-87 18.638525571532995 2.4900685878074017E-4 3.3955480742828205E-5 88-89 20.204161855363687 2.4900685878074017E-4 3.3955480742828205E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGCGCCC 265 7.2759576E-12 15.23569 44-45 CGCGAAT 1200 0.0 13.854455 1 CGGGCAT 1285 0.0 12.198697 1 GGGCGCC 820 0.0 12.165818 42-43 CGAAAAC 5710 0.0 11.313722 1 GCGGGCT 1220 0.0 11.291219 1 CGAAAAT 6160 0.0 11.181239 1 GTGTGCG 1440 0.0 10.883485 8 GTCGCCC 670 5.1088136E-8 10.63461 2 CAAAAAT 9395 0.0 10.617571 1 CGGGAAT 2410 0.0 10.249176 1 TCGGGGG 1840 0.0 9.554483 38-39 CTCGGCT 2995 0.0 9.516058 1 GGGGCGC 965 0.0 9.353242 42-43 CGCGACT 1025 4.2382453E-10 9.268486 1 GGGGGCG 960 0.0 9.156302 40-41 GGGGGGC 1090 0.0 9.154032 40-41 CGCACAC 1205 1.0913936E-11 9.066579 1 CAGGTCC 2690 0.0 9.005764 1 CACTGTA 2130 0.0 8.920222 4 >>END_MODULE