FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l03n02 tsrexp_lib#39.3420000000ce5a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l03n02 tsrexp_lib#39.3420000000ce5a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8835098
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCCC2657.2759576E-1215.2356944-45
CGCGAAT12000.013.8544551
CGGGCAT12850.012.1986971
GGGCGCC8200.012.16581842-43
CGAAAAC57100.011.3137221
GCGGGCT12200.011.2912191
CGAAAAT61600.011.1812391
GTGTGCG14400.010.8834858
GTCGCCC6705.1088136E-810.634612
CAAAAAT93950.010.6175711
CGGGAAT24100.010.2491761
TCGGGGG18400.09.55448338-39
CTCGGCT29950.09.5160581
GGGGCGC9650.09.35324242-43
CGCGACT10254.2382453E-109.2684861
GGGGGCG9600.09.15630240-41
GGGGGGC10900.09.15403240-41
CGCACAC12051.0913936E-119.0665791
CAGGTCC26900.09.0057641
CACTGTA21300.08.9202224
Produced by FastQC (version 0.11.2)