FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l03n02 tsrexp_lib#36.3420000000ce16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l03n02 tsrexp_lib#36.3420000000ce16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7528959
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCCC2950.024.15354244-45
GGGCGCC9000.023.2255842-43
GGGGGCG9050.019.42586140-41
GGGGCGC9900.017.7550942-43
GGGGCCC3000.017.41738944-45
GGGGGGC10300.016.837740-41
TCGGGGG15850.016.33799438-39
GGGCCCG2253.645073E-813.72279144-45
GGGTAGA18000.012.53490828-29
CGGGTAG7250.012.45297514-15
CTCGGGG12900.011.78688336-37
GCGGGCT9300.011.746941
GGGCGGG7700.011.72208312-13
CGGGGGG15150.011.44752938-39
AGGGTAG17750.011.23960428-29
GAGCGGC17000.011.1770099
GTAGGGG12200.011.10134918-19
CACGAAT17400.010.9191941
GGGCCCC2259.522757E-510.55599344-45
GAGGGGC10051.8189894E-1210.3985099
Produced by FastQC (version 0.11.2)