FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l03n02 tsrexp_lib#33.3420000000cdee.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l03n02 tsrexp_lib#33.3420000000cdee.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6495659
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCCC3850.031.4617444-45
GGGCGCC11100.028.88856342-43
TCGGGGG19250.026.65672938-39
GGGGCGC10800.025.95215442-43
GGGGGCG12750.021.98630540-41
CGGGTAG8650.020.32495314-15
CTCGGGG15000.020.27227836-37
GGGGGGC16150.018.82859440-41
GGGTAGA22000.018.4600728-29
GCGCCCG5150.018.44701444-45
AGGGTAG21150.018.41591828-29
GGGCCCG3250.018.2696444-45
GGGCGGG10200.018.1624512-13
GAGCGGC17000.018.161619
GTAGGGG13450.017.31242618-19
GGGGCCC5100.017.23077644-45
CGGGGGG19550.017.0123838-39
GGGGGTC8450.016.58730140-41
AGGGGCG11250.015.622148510-11
AAGGGGC14650.015.5630079
Produced by FastQC (version 0.11.2)