##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C5B16ACXX l03n02 tsrexp_lib#30.3420000000cd74.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 10816036 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.59008910473301 33.0 33.0 33.0 33.0 33.0 2 32.52873612846703 33.0 33.0 33.0 33.0 33.0 3 32.51354488834912 33.0 33.0 33.0 33.0 33.0 4 36.392339670467074 37.0 37.0 37.0 37.0 37.0 5 36.3949132565757 37.0 37.0 37.0 37.0 37.0 6 36.41995764437175 37.0 37.0 37.0 37.0 37.0 7 36.416613073403234 37.0 37.0 37.0 37.0 37.0 8 36.446786142353815 37.0 37.0 37.0 37.0 37.0 9 36.39747131019165 37.0 37.0 37.0 37.0 37.0 10-11 36.38986353225896 37.0 37.0 37.0 37.0 37.0 12-13 36.38974028932596 37.0 37.0 37.0 37.0 37.0 14-15 38.76059653462692 40.0 40.0 40.0 37.0 40.0 16-17 38.748027188518975 40.0 40.0 40.0 37.0 40.0 18-19 38.74169589487313 40.0 40.0 40.0 37.0 40.0 20-21 38.716555862055195 40.0 40.0 40.0 37.0 40.0 22-23 38.73701797959992 40.0 40.0 40.0 37.0 40.0 24-25 38.68493679200033 40.0 40.0 40.0 37.0 40.0 26-27 38.67623707058667 40.0 40.0 40.0 37.0 40.0 28-29 38.62202215303277 40.0 40.0 40.0 37.0 40.0 30-31 38.60221346341673 40.0 40.0 40.0 37.0 40.0 32-33 38.5690610682139 40.0 40.0 40.0 37.0 40.0 34-35 38.53992826022399 40.0 40.0 40.0 37.0 40.0 36-37 38.51373053861877 40.0 40.0 40.0 37.0 40.0 38-39 38.48517885850232 40.0 40.0 40.0 37.0 40.0 40-41 38.43705845653621 40.0 40.0 40.0 37.0 40.0 42-43 38.36429603229871 40.0 37.0 40.0 37.0 40.0 44-45 38.31815116924537 40.0 37.0 40.0 37.0 40.0 46-47 38.22662669576913 40.0 37.0 40.0 37.0 40.0 48-49 38.143077417641734 40.0 37.0 40.0 37.0 40.0 50-51 36.78059790111645 38.5 37.0 38.5 35.0 40.0 52-53 37.10229537882455 38.5 37.0 40.0 33.0 40.0 54-55 37.747624453173046 40.0 37.0 40.0 37.0 40.0 56-57 37.70401184870316 40.0 37.0 40.0 35.0 40.0 58-59 37.515471148579756 40.0 37.0 40.0 33.0 40.0 60-61 37.321124162308635 37.0 37.0 40.0 33.0 40.0 62-63 37.09380118557298 37.0 37.0 40.0 33.0 40.0 64-65 36.828228983335485 37.0 37.0 40.0 33.0 40.0 66-67 36.54465665609841 37.0 37.0 40.0 33.0 40.0 68-69 36.243009684879006 37.0 37.0 37.0 33.0 40.0 70-71 35.976593735449846 37.0 37.0 37.0 33.0 40.0 72-73 35.75279589491011 37.0 37.0 37.0 33.0 38.5 74-75 35.546171998687875 37.0 37.0 37.0 33.0 37.0 76-77 35.37639921871562 37.0 37.0 37.0 33.0 37.0 78-79 35.249965560395694 37.0 37.0 37.0 33.0 37.0 80-81 35.153384289771225 37.0 37.0 37.0 33.0 37.0 82-83 35.07274384071947 37.0 37.0 37.0 33.0 37.0 84-85 35.00821280550471 37.0 37.0 37.0 33.0 37.0 86-87 34.949056659944546 37.0 33.0 37.0 33.0 37.0 88-89 34.8820169422513 37.0 33.0 37.0 33.0 37.0 90-91 34.810061884039584 37.0 33.0 37.0 33.0 37.0 92-93 34.76677943749448 37.0 33.0 37.0 33.0 37.0 94-95 34.707838111855395 37.0 33.0 37.0 33.0 37.0 96-97 34.65228661406083 37.0 33.0 37.0 33.0 37.0 98-99 34.587571685227374 37.0 33.0 37.0 33.0 37.0 100-101 32.732903718145906 35.0 30.0 37.0 27.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.16727127595132885 2306 2 0.18251745133238018 2306 3 0.1787522642972661 2306 4 0.2653382994202502 2306 5 0.25847848410273144 2306 6 0.27932745749686916 2306 7 0.2069494971570478 2306 8 0.2392550797583155 2306 9 0.2453483247520225 2306 10-11 0.24051553581355023 2306 12-13 0.22846344965609688 2306 14-15 0.41017567634136753 2306 16-17 0.44766241229585546 2306 18-19 0.45131171270418236 2306 20-21 0.43210334026817065 2306 22-23 0.5057713546279601 2306 24-25 0.5238843466210241 2306 26-27 0.5183692644967266 2306 28-29 0.5098946778071678 2306 30-31 0.5362082232867849 2306 32-33 0.506969073482658 2306 34-35 0.5143588405511821 2306 36-37 0.5460033991104609 2306 38-39 0.5386861851421116 2306 40-41 0.5230100241258029 2306 42-43 0.5879679494430547 2306 44-45 0.5957720393752624 2306 46-47 0.5789852191951894 2306 48-49 0.5960340679239451 2306 50-51 0.2381174017177088 2306 52-53 0.4103985992868502 2306 54-55 0.6044362836611086 2306 56-57 0.6316158308644688 2306 58-59 0.572994391859801 2306 60-61 0.644173786170164 2306 62-63 0.6224428916036047 2306 64-65 0.6207869336454692 2306 66-67 0.6119455954132818 2306 68-69 0.6321000321876937 2306 70-71 0.6047212187494537 2306 72-73 0.6932905886227871 2306 74-75 0.5974772287382066 2306 76-77 0.59465560235666 2306 78-79 0.5827609807399341 2306 80-81 0.6445655564554755 2306 82-83 0.6695639461557192 2306 84-85 0.6591202569298531 2306 86-87 0.7255025879260586 2306 88-89 0.7574758402318693 2306 90-91 0.7546274600356284 2306 92-93 0.786840514769878 2306 94-95 0.736901404777889 2306 96-97 0.7168963540430795 2306 98-99 0.7424717825831451 2306 100-101 0.5565339882141487 2307 1 0.1382246309626538 2307 2 0.18095191063640215 2307 3 0.18398834924349217 2307 4 0.24613731790042692 2307 5 0.21529969454712727 2307 6 0.24183572145441445 2307 7 0.21149835243053872 2307 8 0.19996112742088457 2307 9 0.21312977079433182 2307 10-11 0.2034392255488342 2307 12-13 0.22341077793675623 2307 14-15 0.3990991754098019 2307 16-17 0.3956961603296065 2307 18-19 0.42344647686792314 2307 20-21 0.4184712817085767 2307 22-23 0.4601764843954115 2307 24-25 0.4359954225002127 2307 26-27 0.37608391496499394 2307 28-29 0.4050795106731613 2307 30-31 0.4227033105065132 2307 32-33 0.48017965466426205 2307 34-35 0.49204315011954947 2307 36-37 0.48371668175128235 2307 38-39 0.47152969117401256 2307 40-41 0.48369130582157993 2307 42-43 0.46002986643250665 2307 44-45 0.46669876320054016 2307 46-47 0.46754614036481 2307 48-49 0.484396446866036 2307 50-51 0.40778893660663584 2307 52-53 0.4890167619629793 2307 54-55 0.5342248714207116 2307 56-57 0.5279279815244493 2307 58-59 0.5319437138525984 2307 60-61 0.5283194224897159 2307 62-63 0.5220192795857841 2307 64-65 0.4928031527631376 2307 66-67 0.48119724872289993 2307 68-69 0.480440297919273 2307 70-71 0.5123671669751175 2307 72-73 0.5924496856079671 2307 74-75 0.5294036686356591 2307 76-77 0.5634980472860533 2307 78-79 0.5711401466553312 2307 80-81 0.5796137516485942 2307 82-83 0.612226069977261 2307 84-85 0.540297396802643 2307 86-87 0.5725247653830152 2307 88-89 0.589890760327954 2307 90-91 0.6088856519749868 2307 92-93 0.6161751288870931 2307 94-95 0.625699623286259 2307 96-97 0.6104756476223727 2307 98-99 0.6204694489866185 2307 100-101 0.6867010082362413 2201 1 0.19030860911972525 2201 2 0.2048352602601753 2201 3 0.17247259259074355 2201 4 0.20990213353420728 2201 5 0.23632298514949923 2201 6 0.2646429029965205 2201 7 0.22574709137086302 2201 8 0.26186414700075034 2201 9 0.24280403155947994 2201 10-11 0.19754331405989234 2201 12-13 0.2229915793241446 2201 14-15 0.4090945157313399 2201 16-17 0.4311810151555875 2201 18-19 0.45237122573676913 2201 20-21 0.4346486642977325 2201 22-23 0.49200724387483064 2201 24-25 0.47802400067211437 2201 26-27 0.4587974741952081 2201 28-29 0.5016974618168248 2201 30-31 0.48838525736251626 2201 32-33 0.4539266985841053 2201 34-35 0.475926050187816 2201 36-37 0.5060741145301009 2201 38-39 0.5371391799110796 2201 40-41 0.4935539499655164 2201 42-43 0.4941063964777044 2201 44-45 0.5221420686322915 2201 46-47 0.4814052496704875 2201 48-49 0.4951792228962333 2201 50-51 0.47088396211444916 2201 52-53 0.4982012670930587 2201 54-55 0.555341358565471 2201 56-57 0.5967109396365231 2201 58-59 0.544902864777832 2201 60-61 0.563500574625543 2201 62-63 0.5135244645484391 2201 64-65 0.548441666967932 2201 66-67 0.5563552725363792 2201 68-69 0.5468912314634906 2201 70-71 0.5607646413532734 2201 72-73 0.5721617096299312 2201 74-75 0.5881680207396656 2201 76-77 0.5958540909621561 2201 78-79 0.6328802766969872 2201 80-81 0.5866018719065949 2201 82-83 0.6133285067286423 2201 84-85 0.6110719125478212 2201 86-87 0.6689079486534197 2201 88-89 0.6469932585661482 2201 90-91 0.6352567204381856 2201 92-93 0.7059255250876149 2201 94-95 0.6920173552002566 2201 96-97 0.6694875971762997 2201 98-99 0.6813554235744803 2201 100-101 0.6193664097252807 1101 1 0.18123354226917598 1101 2 0.1738542034209445 1101 3 0.1854651407994936 1101 4 0.24309561373718225 1101 5 0.24636001736929813 1101 6 0.24242176246873726 1101 7 0.226098572164247 1101 8 0.22927522739279027 1101 9 0.25712525527987395 1101 10-11 0.2684384632160288 1101 12-13 0.23882908002073577 1101 14-15 0.4039340075670168 1101 16-17 0.3945179170320827 1101 18-19 0.40214390214570983 1101 20-21 0.466748475406618 1101 22-23 0.4183997895986593 1101 24-25 0.4447855532604734 1101 26-27 0.48770730024983777 1101 28-29 0.4421869325699461 1101 30-31 0.38512540865259126 1101 32-33 0.4829431825064461 1101 34-35 0.5557899359805987 1101 36-37 0.5076208895227836 1101 38-39 0.48549618216916457 1101 40-41 0.49177005846473776 1101 42-43 0.5454121467076831 1101 44-45 0.5128117987834315 1101 46-47 0.5343215625365687 1101 48-49 0.5424140973610534 1101 50-51 0.48905223473038717 1101 52-53 0.5329430688146957 1101 54-55 0.5709523194189146 1101 56-57 0.546311506052966 1101 58-59 0.5192407301662243 1101 60-61 0.5373296198617439 1101 62-63 0.4898482265635735 1101 64-65 0.5412861402948437 1101 66-67 0.49915849031696524 1101 68-69 0.47397156184966605 1101 70-71 0.5149093860142315 1101 72-73 0.5311248001361477 1101 74-75 0.5227121082896033 1101 76-77 0.5440175808809826 1101 78-79 0.5262995124709633 1101 80-81 0.5640338901633655 1101 82-83 0.5454133820663216 1101 84-85 0.5759527715942454 1101 86-87 0.5742997201181836 1101 88-89 0.6507496128875232 1101 90-91 0.6474875914977147 1101 92-93 0.6718803284688519 1101 94-95 0.6226617202284004 1101 96-97 0.60309534188481 1101 98-99 0.6593724087285082 1101 100-101 0.6815416163345986 2304 1 0.12465856031794686 2304 2 0.16017391039311235 2304 3 0.12269798626456208 2304 4 0.16736555066125192 2304 5 0.20282328079713352 2304 6 0.23631450619172512 2304 7 0.18895698331520094 2304 8 0.22034401682640947 2304 9 0.18236925368189105 2304 10-11 0.2006860882587418 2304 12-13 0.1817704972674008 2304 14-15 0.3465789585254129 2304 16-17 0.3389218776227949 2304 18-19 0.3617796846596235 2304 20-21 0.33373870012715656 2304 22-23 0.3924116146408707 2304 24-25 0.3870530386523896 2304 26-27 0.3886737996302969 2304 28-29 0.3794124310696674 2304 30-31 0.37254559767499273 2304 32-33 0.371455824685448 2304 34-35 0.38436631896970397 2304 36-37 0.44824719071701935 2304 38-39 0.48181554533582727 2304 40-41 0.5034977127914004 2304 42-43 0.5185394438013446 2304 44-45 0.5121097475350638 2304 46-47 0.48715522212657447 2304 48-49 0.49716835923011615 2304 50-51 0.3932508813300686 2304 52-53 0.47964500592126313 2304 54-55 0.5081155107815434 2304 56-57 0.5428909254089049 2304 58-59 0.4960831867121698 2304 60-61 0.52850538679359 2304 62-63 0.5168733079667263 2304 64-65 0.5494779153305984 2304 66-67 0.5250622867983878 2304 68-69 0.5115579871256273 2304 70-71 0.509297420738342 2304 72-73 0.4871657348292544 2304 74-75 0.511046761100026 2304 76-77 0.5316840048677776 2304 78-79 0.5203461771475801 2304 80-81 0.4963410520095408 2304 82-83 0.5355962533107217 2304 84-85 0.5542262221537584 2304 86-87 0.5861550533371656 2304 88-89 0.5785338042638699 2304 90-91 0.5910051772507998 2304 92-93 0.6056788735424021 2304 94-95 0.6219963023293644 2304 96-97 0.6141380946474086 2304 98-99 0.6083586330467057 2304 100-101 0.5808271422202296 2202 1 0.11069728717809824 2202 2 0.11217284769891478 2202 3 0.08533678331229311 2202 4 0.12667631076937624 2202 5 0.11373283944602974 2202 6 0.16542883254643215 2202 7 0.11586949438035532 2202 8 0.19746778593633252 2202 9 0.1619664581813609 2202 10-11 0.1477905210182655 2202 12-13 0.15178387608970922 2202 14-15 0.31233114036029974 2202 16-17 0.2972704402227677 2202 18-19 0.3459849987161334 2202 20-21 0.2936866650714194 2202 22-23 0.32755648357341727 2202 24-25 0.3144347782432746 2202 26-27 0.38404220577562853 2202 28-29 0.3442715051570886 2202 30-31 0.3462674279535136 2202 32-33 0.38863451064064947 2202 34-35 0.38429741217976954 2202 36-37 0.36259141251857585 2202 38-39 0.37413350863701567 2202 40-41 0.3881085083592666 2202 42-43 0.408054944535607 2202 44-45 0.4124222341553221 2202 46-47 0.4007004014507345 2202 48-49 0.41398648299666974 2202 50-51 0.41859327452179684 2202 52-53 0.4561746781071818 2202 54-55 0.4237778082283441 2202 56-57 0.4179525774080233 2202 58-59 0.49357583769140945 2202 60-61 0.5150834931706001 2202 62-63 0.47569520678609223 2202 64-65 0.5159837903388791 2202 66-67 0.4659560880050435 2202 68-69 0.4354701801875933 2202 70-71 0.43079068540153287 2202 72-73 0.44837404814254 2202 74-75 0.48623981355371626 2202 76-77 0.4601177623151429 2202 78-79 0.5203264711962419 2202 80-81 0.5180223465789311 2202 82-83 0.4985202285920991 2202 84-85 0.5083599617065815 2202 86-87 0.5516830045000702 2202 88-89 0.5705317516978354 2202 90-91 0.5860213463772652 2202 92-93 0.5993294403675407 2202 94-95 0.6324957045638726 2202 96-97 0.6269556175379662 2202 98-99 0.6429799402849952 2202 100-101 0.7696003122655739 1102 1 0.1323564192444664 1102 2 0.1553851919588638 1102 3 0.15905446487744967 1102 4 0.1816652990433525 1102 5 0.18357773149038792 1102 6 0.145912835201635 1102 7 0.17331136304733263 1102 8 0.19554026612541264 1102 9 0.18340062021309933 1102 10-11 0.16466617122007676 1102 12-13 0.1899267034995944 1102 14-15 0.298004066018855 1102 16-17 0.28451645067633535 1102 18-19 0.2871630340109448 1102 20-21 0.33572030510380557 1102 22-23 0.3091792531835367 1102 24-25 0.33600119523575955 1102 26-27 0.3439229552651071 1102 28-29 0.33050865241717275 1102 30-31 0.34370913269884085 1102 32-33 0.3502287214581159 1102 34-35 0.38332851937442314 1102 36-37 0.3481383834370533 1102 38-39 0.40081971178295817 1102 40-41 0.39385204119517425 1102 42-43 0.3768748295884521 1102 44-45 0.35996589319434236 1102 46-47 0.3413558495851845 1102 48-49 0.3519824763543582 1102 50-51 0.3656428422736724 1102 52-53 0.36748853324449016 1102 54-55 0.38616248562806277 1102 56-57 0.36185473373926413 1102 58-59 0.3770926556378811 1102 60-61 0.38140445903002984 1102 62-63 0.36677984813961473 1102 64-65 0.36363961151923974 1102 66-67 0.3929228620817824 1102 68-69 0.3484495537122001 1102 70-71 0.3370113875126961 1102 72-73 0.4423186668020165 1102 74-75 0.40855925179590713 1102 76-77 0.4237653897315212 1102 78-79 0.4208265398280773 1102 80-81 0.43004766303462816 1102 82-83 0.469765789668557 1102 84-85 0.46840354830547426 1102 86-87 0.4971663589196922 1102 88-89 0.4642670142729841 1102 90-91 0.4087272340286816 1102 92-93 0.4322773674293643 1102 94-95 0.48821397856502813 1102 96-97 0.5029659999042408 1102 98-99 0.49011571591832137 1102 100-101 0.5655239039178923 2305 1 0.10762939657443837 2305 2 0.11577219441975473 2305 3 0.11914359206655689 2305 4 0.14572468301444275 2305 5 0.13106635390119692 2305 6 0.1926671370848112 2305 7 0.13825571870036413 2305 8 0.18325676225371978 2305 9 0.17751719415938538 2305 10-11 0.15435645882462268 2305 12-13 0.17564656123948197 2305 14-15 0.3168025313294507 2305 16-17 0.3204364265970696 2305 18-19 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-1.0594599707827896 1213 62-63 -1.0258797997205065 1213 64-65 -0.9403754589838513 1213 66-67 -1.0800406917045038 1213 68-69 -1.1204262299859664 1213 70-71 -1.0600216432808267 1213 72-73 -1.0184550218561483 1213 74-75 -1.1038346261737573 1213 76-77 -1.0936002369797322 1213 78-79 -1.1320959516660238 1213 80-81 -1.1746464570608168 1213 82-83 -1.1692058755096966 1213 84-85 -1.1139358279450704 1213 86-87 -1.2139044594894628 1213 88-89 -1.2689437432535087 1213 90-91 -1.4761667086794645 1213 92-93 -1.2985803611483675 1213 94-95 -1.1996645699347752 1213 96-97 -1.3819341920886714 1213 98-99 -1.3784980636288324 1213 100-101 -1.2728341366749518 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10209.0 3 777.0 4 540.0 5 425.0 6 442.0 7 1345.0 8 2468.0 9 4635.0 10 10782.0 11 17320.0 12 15597.0 13 12504.0 14 11534.0 15 10838.0 16 11258.0 17 12070.0 18 13459.0 19 14795.0 20 16488.0 21 18473.0 22 21111.0 23 23637.0 24 26810.0 25 30620.0 26 35144.0 27 40743.0 28 50107.0 29 62290.0 30 78367.0 31 102320.0 32 136521.0 33 189502.0 34 267815.0 35 406909.0 36 843938.0 37 4038339.0 38 4274421.0 39 1483.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 28.052788261852353 14.269724543695878 19.487994705953803 38.18949248849797 2 25.359836512685373 24.97166153222883 30.279018646545154 19.389483308540644 3 23.412480341159345 27.560463317260638 29.530365248020786 19.49669109355923 4 25.592825707640237 31.37803912230261 21.231114301792314 21.798020868264842 5 27.076111248597478 34.02518139173421 21.46019593797689 17.438511421691423 6 19.187060371958673 37.59114242703468 22.427419774391307 20.794377426615338 7 18.147270994359143 16.18597754469843 43.15386199046601 22.51288947047641 8 21.98376749034448 21.77692467265007 27.85171633254771 28.387591504457742 9 24.283084144368523 23.258509991072227 26.74602610346517 25.712379761094084 10-11 24.261251683768712 31.58704755714264 21.66384758409034 22.487853174998307 12-13 22.94170899856709 25.60568728993501 27.799548498804334 23.653055212693562 14-15 22.97547445241957 26.938401628585623 27.669700279317066 22.416423639677745 16-17 23.92603116536824 27.314584134113883 26.680467794431134 22.07891690608674 18-19 23.80587831485178 27.517085370855643 26.772805877898158 21.904230436394418 20-21 23.86415197033376 27.7706076676443 26.578770024070543 21.786470337951396 22-23 24.09849200581816 27.582146315647893 26.56989952030047 21.749462158233477 24-25 23.45099915883312 28.15519991880943 26.559821002947974 21.833979919409472 26-27 23.718678350178806 28.275444370955572 26.369555365864034 21.636321913001588 28-29 23.843554854120853 27.67096629801528 26.658095553543316 21.827383294320555 30-31 23.65898461530345 27.633735940261438 26.726432255276773 21.980847189158336 32-33 23.643873202571424 27.874916050186137 26.589001613965547 21.892209133276896 34-35 23.80892246053358 27.761496790261187 26.547182061107133 21.8823986880981 36-37 23.37041725639829 27.703043204972367 26.90889031820463 22.017649220424715 38-39 23.682693682698776 27.68652734139066 26.632743394991515 21.998035580919055 40-41 24.26655579832959 27.498539782312836 26.474375303985298 21.76052911537227 42-43 23.98250008345486 27.481187006340207 26.748363069421988 21.787949840782947 44-45 24.237956553221366 27.613022275554126 26.459359827677176 21.689661343547336 46-47 24.193406612532037 27.47951899179593 26.43581600644213 21.891258389229904 48-49 23.798313065432396 27.541913863573882 26.56371923042627 22.09605384056745 50-51 24.38903932508391 27.684516182724572 26.34399036278508 21.58245412940644 52-53 24.37616933029777 27.60233950068737 26.45918512203387 21.56230604698099 54-55 24.173784466720353 27.690884773091202 26.330963818621058 21.804366941567395 56-57 24.43930772795857 27.62971308199056 26.46994893594769 21.461030254103182 58-59 24.680574827620536 27.883752086809615 26.096890909963538 21.338782175606312 60-61 24.521690812051066 28.087133966837946 26.04017932784963 21.350995893261356 62-63 24.873038896638658 28.12946249565883 25.725497473040793 21.272001134661718 64-65 25.093422831775957 28.06373223408783 25.74042751813299 21.10241741600322 66-67 24.96055454930744 28.15709614101456 25.80138800227061 21.080961307407396 68-69 25.42505293564365 28.11554569820923 25.509618525973444 20.949782840173672 70-71 25.685328637346238 28.029151373951965 25.432090721791457 20.85342926691034 72-73 25.641395498902963 28.094404717412992 25.51047399775243 20.753725785931614 74-75 26.039353678762055 28.194106535172647 25.224890299362578 20.54164948670272 76-77 26.348103902033454 27.99197611913759 25.187859434832248 20.472060543996708 78-79 26.34539644319826 28.10410992791353 25.160366780655686 20.39012684823253 80-81 26.85677670024423 28.0924663072639 24.768538468367332 20.282218524124534 82-83 27.08978323158432 27.97127890343118 24.84672160778605 20.09221625719845 84-85 27.090385391380746 28.037757139743803 24.866130743808284 20.00572672506717 86-87 27.471152273886123 28.09042051248633 24.537216145775602 19.90121106785195 88-89 27.838156508965874 27.999360516089805 24.559287925223707 19.603195049720615 90-91 27.870544456150064 28.08046791089035 24.545341378377607 19.503646254581977 92-93 28.253589171110356 28.05929447714058 24.312703520348087 19.374412831400978 94-95 28.57212544582854 28.000089416787333 24.24857385546628 19.179211281917848 96-97 28.50274704931471 28.166109012632095 24.20878629996394 19.12235763808926 98-99 28.89596904705568 28.238115320060906 24.05112596527225 18.814789667611162 100-101 29.084889003256215 28.27090717510542 23.865862798665418 18.778341022972945 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 8640.0 1 4743.0 2 677.5 3 441.5 4 361.0 5 311.5 6 278.0 7 286.5 8 284.5 9 283.0 10 311.0 11 344.5 12 353.5 13 374.5 14 415.5 15 488.5 16 553.5 17 592.5 18 724.0 19 880.0 20 1052.0 21 1282.0 22 1617.0 23 2154.5 24 2970.0 25 4344.0 26 6474.5 27 9321.0 28 13455.5 29 19381.5 30 25754.0 31 33793.0 32 43829.0 33 54964.0 34 69289.0 35 88977.0 36 117213.5 37 153523.5 38 198477.0 39 253358.5 40 320743.0 41 397077.5 42 478734.5 43 562736.5 44 639461.5 45 703865.0 46 751329.0 47 775702.5 48 770278.5 49 737838.0 50 682090.0 51 605029.0 52 514803.5 53 426967.5 54 341083.0 55 261043.0 56 197857.0 57 148046.5 58 109356.5 59 79574.0 60 55656.5 61 37376.5 62 26668.0 63 19393.5 64 13629.5 65 10262.0 66 7714.0 67 5572.0 68 4513.5 69 3662.5 70 2822.0 71 2037.5 72 1234.5 73 809.5 74 766.5 75 627.0 76 535.5 77 293.5 78 114.0 79 77.0 80 67.5 81 57.0 82 43.0 83 34.0 84 28.0 85 28.5 86 26.5 87 19.5 88 16.5 89 17.0 90 14.5 91 12.0 92 12.0 93 8.0 94 4.0 95 2.5 96 3.0 97 3.5 98 2.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.13376434767783688 2 0.13372736555240755 3 0.132026187782659 4 0.13995885368724734 5 0.13835937676242943 6 0.12424145037978794 7 0.12407503081535601 8 0.12155100075480517 9 0.12751436848028244 10-11 0.0917156710647043 12-13 0.0993802165599301 14-15 0.15237560230014027 16-17 0.15418772644617676 18-19 0.15408140283556748 20-21 0.18546998179369967 22-23 0.15799226259971766 24-25 0.15488114129797645 26-27 0.14293129201862864 28-29 0.15331864649858784 30-31 0.10933303106609484 32-33 0.12436164228743322 34-35 0.13797568721110026 36-37 0.140707741727191 38-39 0.14226561376090094 40-41 0.1742274156631875 42-43 0.15518162106708963 44-45 0.1378786091318483 46-47 0.1295899902700028 48-49 0.15206125423399108 50-51 0.12707982850648794 52-53 0.12615989813643372 54-55 0.11220376855254549 56-57 0.10603237637152835 58-59 0.11799609394791215 60-61 0.11736739781561378 62-63 0.10219548085823678 64-65 0.11897612027178903 66-67 0.10979068486828261 68-69 0.11637812596037957 70-71 0.09968531909472195 72-73 0.10604624466856434 74-75 0.13809587911874555 76-77 0.16890198960136596 78-79 0.1359370475468092 80-81 0.12467599035358241 82-83 0.114732421378775 84-85 0.11376164058625544 86-87 0.11835666967084799 88-89 0.12550808817574202 90-91 0.14268166267198076 92-93 0.11650756339938219 94-95 0.1173766433469711 96-97 0.11505139221060284 98-99 0.10638370656310685 100-101 0.0988948261636703 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.0816036E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.72902157029717 #Duplication Level Percentage of deduplicated Percentage of total 1 70.24306940052173 32.82389905180861 2 14.529758976959187 13.579228412910895 3 5.638531501675816 7.904491804998279 4 2.574204291864909 4.811601915236275 5 1.7567009572828547 4.104445846271611 6 1.051750782909543 2.948837101269418 7 0.6621079193400395 2.16577586712937 8 0.6594996708172268 2.465421947457766 9 0.3939220550538505 1.6566832986864455 >10 2.3043287810809248 19.100952744424276 >50 0.13791637363135015 4.449375500861754 >100 0.04743628149289035 3.7148518020161223 >500 7.515198587428947E-4 0.2400546529275327 >1k 2.1487510939749768E-5 0.03438005400157609 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 2.0340168986123937E-4 0.0 0.0 2 2.3113828393322655E-4 0.0 0.0 3 2.496293466478847E-4 0.0 0.0 4 3.235935975065172E-4 0.0 0.0 5 3.513301915785044E-4 0.0 9.245531357329063E-6 6 3.7906678565049156E-4 0.0 9.245531357329063E-6 7 3.7906678565049156E-4 0.0 9.245531357329063E-6 8 4.2529444243713684E-4 0.0 9.245531357329063E-6 9 4.715220992237822E-4 0.0 9.245531357329063E-6 10-11 5.593546471184083E-4 1.3868297035993594E-5 9.245531357329063E-6 12-13 6.564327263703634E-4 1.8491062714658126E-5 9.245531357329063E-6 14-15 7.211514458716668E-4 1.8491062714658126E-5 9.245531357329063E-6 16-17 8.320978221596155E-4 1.8491062714658126E-5 9.245531357329063E-6 18-19 0.0010863499344861648 2.7736594071987187E-5 9.245531357329063E-6 20-21 0.0024223292156202144 4.1604891107980784E-5 9.245531357329063E-6 22-23 0.00895891988525186 4.622765678664531E-5 9.245531357329063E-6 24-25 0.01710885577673743 5.085042246530984E-5 9.245531357329063E-6 26-27 0.03267833058247957 5.5473188143974374E-5 9.245531357329063E-6 28-29 0.06941082666514793 6.009595382263891E-5 9.245531357329063E-6 30-31 0.12795815398543423 7.858701653729703E-5 1.8491062714658126E-5 32-33 0.2112696370463264 8.320978221596155E-5 1.8491062714658126E-5 34-35 0.30152913692225136 8.320978221596155E-5 1.8491062714658126E-5 36-37 0.42140669650137996 9.245531357329062E-5 1.8491062714658126E-5 38-39 0.5784882742624007 1.0170084493061968E-4 1.8491062714658126E-5 40-41 0.7780068409535619 1.1094637628794875E-4 1.8491062714658126E-5 42-43 1.0283758301100328 1.1094637628794875E-4 2.3113828393322655E-5 44-45 1.3184728675089468 1.1094637628794875E-4 2.7736594071987187E-5 46-47 1.65513040082337 1.1094637628794875E-4 2.7736594071987187E-5 48-49 2.0379277583765436 1.1094637628794875E-4 2.7736594071987187E-5 50-51 2.479753210880585 1.1556914196661329E-4 2.7736594071987187E-5 52-53 2.9899123856466456 1.2019190764527781E-4 2.7736594071987187E-5 54-55 3.5569916742141023 1.2019190764527781E-4 2.7736594071987187E-5 56-57 4.184527492327134 1.2019190764527781E-4 2.7736594071987187E-5 58-59 4.877184210555512 1.2943743900260688E-4 2.7736594071987187E-5 60-61 5.639945170300838 1.2943743900260688E-4 2.7736594071987187E-5 62-63 6.468030431851373 1.2943743900260688E-4 2.7736594071987187E-5 64-65 7.361245839048612 1.2943743900260688E-4 2.7736594071987187E-5 66-67 8.317922573482559 1.2943743900260688E-4 2.7736594071987187E-5 68-69 9.350366437389816 1.4792850171726498E-4 2.7736594071987187E-5 70-71 10.449156234317268 1.5717403307459406E-4 2.7736594071987187E-5 72-73 11.622881062895871 1.5717403307459406E-4 2.7736594071987187E-5 74-75 12.863247681498102 1.5717403307459406E-4 2.7736594071987187E-5 76-77 14.164038470286156 1.756650957892522E-4 3.235935975065172E-5 78-79 15.539140217358744 1.756650957892522E-4 3.698212542931625E-5 80-81 16.988086023382316 1.756650957892522E-4 3.698212542931625E-5 82-83 18.494733190606986 1.756650957892522E-4 3.698212542931625E-5 84-85 20.063320795160074 1.756650957892522E-4 3.698212542931625E-5 86-87 21.68662345428584 1.756650957892522E-4 3.698212542931625E-5 88-89 23.3636611416604 1.756650957892522E-4 3.698212542931625E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGCGCCC 555 0.0 32.095642 44-45 GGGCGCC 2010 0.0 30.016893 42-43 TCGGGGG 3510 0.0 26.735634 38-39 GGGGCGC 1925 0.0 26.283121 42-43 GGGGGCG 2130 0.0 23.980452 40-41 CTCGGGG 2535 0.0 22.773712 36-37 GTAGGGG 1985 0.0 21.306067 18-19 GAGCGGC 2770 0.0 20.920631 9 GGGCCCG 500 0.0 20.900682 44-45 CGGGTAG 1435 0.0 20.860619 14-15 GGGGGGC 2725 0.0 20.226463 40-41 GGGTAGA 3580 0.0 19.636831 28-29 CGGGGGT 1445 0.0 19.236166 38-39 GGGCGGG 1745 0.0 18.10294 12-13 AGGGTAG 3570 0.0 18.0952 28-29 GATCGGG 4060 0.0 17.90021 1 GGGGTCG 1605 0.0 17.466482 40-41 GCGCCCG 885 0.0 17.444073 44-45 GGGGGTC 1705 0.0 17.278091 40-41 AAGGGGC 2145 0.0 16.608433 9 >>END_MODULE