FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l03n02 tsrexp_lib#30.3420000000cd74.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l03n02 tsrexp_lib#30.3420000000cd74.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10816036
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCCC5550.032.09564244-45
GGGCGCC20100.030.01689342-43
TCGGGGG35100.026.73563438-39
GGGGCGC19250.026.28312142-43
GGGGGCG21300.023.98045240-41
CTCGGGG25350.022.77371236-37
GTAGGGG19850.021.30606718-19
GAGCGGC27700.020.9206319
GGGCCCG5000.020.90068244-45
CGGGTAG14350.020.86061914-15
GGGGGGC27250.020.22646340-41
GGGTAGA35800.019.63683128-29
CGGGGGT14450.019.23616638-39
GGGCGGG17450.018.1029412-13
AGGGTAG35700.018.095228-29
GATCGGG40600.017.900211
GGGGTCG16050.017.46648240-41
GCGCCCG8850.017.44407344-45
GGGGGTC17050.017.27809140-41
AAGGGGC21450.016.6084339
Produced by FastQC (version 0.11.2)