##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C5B16ACXX l03n02 tsrexp_lib#25.3420000000cd24.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 11408286 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.628202781732504 33.0 33.0 33.0 33.0 33.0 2 32.58240747120119 33.0 33.0 33.0 33.0 33.0 3 32.571900984950766 33.0 33.0 33.0 33.0 33.0 4 36.46267081663275 37.0 37.0 37.0 37.0 37.0 5 36.461447232301154 37.0 37.0 37.0 37.0 37.0 6 36.47614383089625 37.0 37.0 37.0 37.0 37.0 7 36.48744509034924 37.0 37.0 37.0 37.0 37.0 8 36.50277535117896 37.0 37.0 37.0 37.0 37.0 9 36.471947319693776 37.0 37.0 37.0 37.0 37.0 10-11 36.459827795341035 37.0 37.0 37.0 37.0 37.0 12-13 36.45933389993905 37.0 37.0 37.0 37.0 37.0 14-15 38.842883716274294 40.0 40.0 40.0 37.0 40.0 16-17 38.830558551915686 40.0 40.0 40.0 37.0 40.0 18-19 38.82554566040858 40.0 40.0 40.0 37.0 40.0 20-21 38.79967455233854 40.0 40.0 40.0 37.0 40.0 22-23 38.80143651728226 40.0 40.0 40.0 37.0 40.0 24-25 38.7645332085819 40.0 40.0 40.0 37.0 40.0 26-27 38.75091700891791 40.0 40.0 40.0 37.0 40.0 28-29 38.705878034614486 40.0 40.0 40.0 37.0 40.0 30-31 38.68923947032884 40.0 40.0 40.0 37.0 40.0 32-33 38.65478521488679 40.0 40.0 40.0 37.0 40.0 34-35 38.61949025471486 40.0 40.0 40.0 37.0 40.0 36-37 38.59706957732301 40.0 40.0 40.0 37.0 40.0 38-39 38.562610281684734 40.0 40.0 40.0 37.0 40.0 40-41 38.51207644163198 40.0 40.0 40.0 37.0 40.0 42-43 38.44928414312194 40.0 38.5 40.0 37.0 40.0 44-45 38.39902462122706 40.0 37.0 40.0 37.0 40.0 46-47 38.31595464910329 40.0 37.0 40.0 37.0 40.0 48-49 38.226649910424754 40.0 37.0 40.0 37.0 40.0 50-51 36.870637228063885 38.5 37.0 38.5 35.0 40.0 52-53 37.193659108826694 38.5 37.0 40.0 33.0 40.0 54-55 37.85073379997661 40.0 37.0 40.0 37.0 40.0 56-57 37.80509438490585 40.0 37.0 40.0 37.0 40.0 58-59 37.62828412611675 40.0 37.0 40.0 35.0 40.0 60-61 37.42703303546212 38.5 37.0 40.0 33.0 40.0 62-63 37.20512993801172 37.0 37.0 40.0 33.0 40.0 64-65 36.9476512510293 37.0 37.0 40.0 33.0 40.0 66-67 36.67060262163834 37.0 37.0 40.0 33.0 40.0 68-69 36.38461868855672 37.0 37.0 37.0 33.0 40.0 70-71 36.125381455198436 37.0 37.0 37.0 33.0 40.0 72-73 35.90392242094912 37.0 37.0 37.0 33.0 40.0 74-75 35.693789978617296 37.0 37.0 37.0 33.0 37.0 76-77 35.51721577632258 37.0 37.0 37.0 33.0 37.0 78-79 35.38877067072126 37.0 37.0 37.0 33.0 37.0 80-81 35.28952701571472 37.0 37.0 37.0 33.0 37.0 82-83 35.20844257410798 37.0 37.0 37.0 33.0 37.0 84-85 35.142476266811684 37.0 37.0 37.0 33.0 37.0 86-87 35.084027039644695 37.0 37.0 37.0 33.0 37.0 88-89 35.01702810571194 37.0 33.0 37.0 33.0 37.0 90-91 34.94922396756182 37.0 33.0 37.0 33.0 37.0 92-93 34.909566827128984 37.0 33.0 37.0 33.0 37.0 94-95 34.8534124232159 37.0 33.0 37.0 33.0 37.0 96-97 34.80649371868833 37.0 33.0 37.0 33.0 37.0 98-99 34.754887938468585 37.0 33.0 37.0 33.0 37.0 100-101 32.942409315474734 35.0 30.0 37.0 27.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.1875945112357158 2306 2 0.22527049984599756 2306 3 0.21590198583106712 2306 4 0.2871903207344815 2306 5 0.27639874226199623 2306 6 0.27099719141024536 2306 7 0.2548023511807287 2306 8 0.2703483938968887 2306 9 0.2893292860955583 2306 10-11 0.28090017466372075 2306 12-13 0.2749509365197653 2306 14-15 0.5070194091621048 2306 16-17 0.4964172971921741 2306 18-19 0.4683884533494833 2306 20-21 0.47355886099174427 2306 22-23 0.5183448710573373 2306 24-25 0.5607247278984246 2306 26-27 0.5102491744836115 2306 28-29 0.5400685430913015 2306 30-31 0.5227627866528977 2306 32-33 0.5362370268076333 2306 34-35 0.5691501711579008 2306 36-37 0.5699467927762996 2306 38-39 0.5278694022155648 2306 40-41 0.5380403561813836 2306 42-43 0.6146515443064686 2306 44-45 0.621212343984169 2306 46-47 0.6225301251552224 2306 48-49 0.6325537638716696 2306 50-51 0.2840947930489435 2306 52-53 0.41713644528803684 2306 54-55 0.611918021818326 2306 56-57 0.620517940567602 2306 58-59 0.6520838242553495 2306 60-61 0.6721388683059146 2306 62-63 0.6719643951970937 2306 64-65 0.6571192808828741 2306 66-67 0.6715476578932709 2306 68-69 0.6723860047668282 2306 70-71 0.6829793286324488 2306 72-73 0.6946063289662234 2306 74-75 0.6461749862538895 2306 76-77 0.6532606427061438 2306 78-79 0.6675082114095332 2306 80-81 0.7297492478569936 2306 82-83 0.7212118958085441 2306 84-85 0.721909626706811 2306 86-87 0.7601229425737088 2306 88-89 0.7874835897713197 2306 90-91 0.7567893166749116 2306 92-93 0.8162729858653464 2306 94-95 0.8260335545986806 2306 96-97 0.8089799494757486 2306 98-99 0.7820639239328457 2306 100-101 0.676163046605275 2307 1 0.1553466951898841 2307 2 0.19479536190016944 2307 3 0.20462051074687793 2307 4 0.23957541543730798 2307 5 0.23483944317811734 2307 6 0.2450661549225046 2307 7 0.19059802065210363 2307 8 0.20153428032373455 2307 9 0.2336985975048833 2307 10-11 0.21990650792579913 2307 12-13 0.23095194543200392 2307 14-15 0.4044306104319446 2307 16-17 0.4144817647874319 2307 18-19 0.46463957119956234 2307 20-21 0.4501470612160503 2307 22-23 0.47847050552034176 2307 24-25 0.44340133578656094 2307 26-27 0.41149427952817774 2307 28-29 0.464169794528452 2307 30-31 0.4697125333646568 2307 32-33 0.44920248500213233 2307 34-35 0.5153204149310113 2307 36-37 0.49885470636385065 2307 38-39 0.49157220869118845 2307 40-41 0.5349319523259268 2307 42-43 0.5137375017903523 2307 44-45 0.5073492864475071 2307 46-47 0.5205497818401383 2307 48-49 0.5532928718063914 2307 50-51 0.47796701991727986 2307 52-53 0.5321445821846922 2307 54-55 0.5000915075947887 2307 56-57 0.5179123797806184 2307 58-59 0.5181403506734696 2307 60-61 0.5519461309500144 2307 62-63 0.5182243837885352 2307 64-65 0.5152474993737854 2307 66-67 0.5414353237444587 2307 68-69 0.510345400045658 2307 70-71 0.5598439650948066 2307 72-73 0.6033156431369875 2307 74-75 0.634341471102104 2307 76-77 0.6072054059000109 2307 78-79 0.595401218175887 2307 80-81 0.6355662737831622 2307 82-83 0.6703441161551069 2307 84-85 0.6177500710589001 2307 86-87 0.6232901210956783 2307 88-89 0.6481054101054298 2307 90-91 0.6682260007437435 2307 92-93 0.6568085612240324 2307 94-95 0.6708522961974666 2307 96-97 0.6318427675603644 2307 98-99 0.6691032013558882 2307 100-101 0.751778631330005 2201 1 0.2168662717135703 2201 2 0.21427899757375712 2201 3 0.2335475261883886 2201 4 0.28966012798046137 2201 5 0.28444521745624485 2201 6 0.2971293490174247 2201 7 0.282837068880589 2201 8 0.29226589227647537 2201 9 0.2727385674350842 2201 10-11 0.26225062845490044 2201 12-13 0.2890542108195504 2201 14-15 0.4876348449291541 2201 16-17 0.4937588841422169 2201 18-19 0.5386510075590607 2201 20-21 0.5251884910999749 2201 22-23 0.5375156507588201 2201 24-25 0.5159085138369406 2201 26-27 0.5164353488105107 2201 28-29 0.5432427762620762 2201 30-31 0.554690177096397 2201 32-33 0.5827516098856691 2201 34-35 0.5461170925309844 2201 36-37 0.5879868871767755 2201 38-39 0.5916959375253015 2201 40-41 0.5898945320804287 2201 42-43 0.6007052796181682 2201 44-45 0.6241410763763611 2201 46-47 0.6584206713168541 2201 48-49 0.618196650454415 2201 50-51 0.6022561731723712 2201 52-53 0.6611233133210703 2201 54-55 0.7047909641749541 2201 56-57 0.6814534297209747 2201 58-59 0.6661682499801955 2201 60-61 0.698182425605431 2201 62-63 0.6669014191286919 2201 64-65 0.6941213036067211 2201 66-67 0.6655688397187873 2201 68-69 0.6816666523423152 2201 70-71 0.6418390559879796 2201 72-73 0.6505117086398187 2201 74-75 0.6710519291796189 2201 76-77 0.6728737036182366 2201 78-79 0.7182890335444228 2201 80-81 0.700001208883684 2201 82-83 0.6879375471277598 2201 84-85 0.7224767210633232 2201 86-87 0.752999763785887 2201 88-89 0.7678193091938113 2201 90-91 0.714470358710571 2201 92-93 0.7412420041342145 2201 94-95 0.7836433169337624 2201 96-97 0.7966647249917358 2201 98-99 0.8066494397317925 2201 100-101 0.7915428245443934 1101 1 0.21688611903037014 1101 2 0.2149457571552702 1101 3 0.22762685106206249 1101 4 0.266972990938271 1101 5 0.2546869224907198 1101 6 0.25617551343523814 1101 7 0.2456486000834417 1101 8 0.26802544541487805 1101 9 0.29091338024916524 1101 10-11 0.28615657451988596 1101 12-13 0.25685701140826467 1101 14-15 0.44287164767637677 1101 16-17 0.44557900805710204 1101 18-19 0.4459828011816569 1101 20-21 0.44848789213735785 1101 22-23 0.4354257944577995 1101 24-25 0.4718939831017579 1101 26-27 0.4702724195406134 1101 28-29 0.440914127336562 1101 30-31 0.386983253749527 1101 32-33 0.46260913040890017 1101 34-35 0.48604485123819074 1101 36-37 0.5137185768876478 1101 38-39 0.4977824039046084 1101 40-41 0.4961693458207108 1101 42-43 0.5360274500854487 1101 44-45 0.5491853322173839 1101 46-47 0.55512599100053 1101 48-49 0.5094361661093174 1101 50-51 0.4534620336586741 1101 52-53 0.5404603012070055 1101 54-55 0.5729434193542033 1101 56-57 0.5717959936738097 1101 58-59 0.5598447772349076 1101 60-61 0.570099714632498 1101 62-63 0.525712348737315 1101 64-65 0.5431608478417616 1101 66-67 0.5483684309228636 1101 68-69 0.5321587996411594 1101 70-71 0.5564656573907882 1101 72-73 0.5084420422055942 1101 74-75 0.5536206483087653 1101 76-77 0.5854344382453505 1101 78-79 0.6026703640115088 1101 80-81 0.6068866400269499 1101 82-83 0.5783519473411118 1101 84-85 0.5467938594272042 1101 86-87 0.5758223073092097 1101 88-89 0.6140662073154743 1101 90-91 0.6941914546588208 1101 92-93 0.6937277666247539 1101 94-95 0.660194347987165 1101 96-97 0.6074923772144061 1101 98-99 0.6785015450249006 1101 100-101 0.6762096807161768 2304 1 0.15600937789830027 2304 2 0.16438674848658508 2304 3 0.20065614296557044 2304 4 0.24677887343528937 2304 5 0.20859225841404339 2304 6 0.2514134830675232 2304 7 0.22637391712821398 2304 8 0.24486326256170088 2304 9 0.22635780187521704 2304 10-11 0.21159036989420343 2304 12-13 0.2125159614818415 2304 14-15 0.3528264107429919 2304 16-17 0.38170578325975413 2304 18-19 0.39799093981027767 2304 20-21 0.40408757196814094 2304 22-23 0.4165646686403477 2304 24-25 0.43924031852076695 2304 26-27 0.43529363436234547 2304 28-29 0.42833140805864645 2304 30-31 0.407324958082242 2304 32-33 0.4438904886850352 2304 34-35 0.45948189101064685 2304 36-37 0.4704067880913101 2304 38-39 0.49345653972092407 2304 40-41 0.48601042620655477 2304 42-43 0.4929923080912175 2304 44-45 0.5345203825821727 2304 46-47 0.4942479153066941 2304 48-49 0.4985284788451807 2304 50-51 0.4198908186171266 2304 52-53 0.4692176263809813 2304 54-55 0.531937439261462 2304 56-57 0.5555089234733472 2304 58-59 0.5085214571969487 2304 60-61 0.53694621742126 2304 62-63 0.5712281270254707 2304 64-65 0.5514927524089757 2304 66-67 0.537491517082799 2304 68-69 0.5648604299418096 2304 70-71 0.5413776516782391 2304 72-73 0.4791278436617361 2304 74-75 0.5103229116627048 2304 76-77 0.5298259189055088 2304 78-79 0.531115558741341 2304 80-81 0.48852404367944047 2304 82-83 0.5426877469445728 2304 84-85 0.4770641066131631 2304 86-87 0.5313210314681669 2304 88-89 0.5434990562499991 2304 90-91 0.583914810996859 2304 92-93 0.6401170862369057 2304 94-95 0.6494339335382264 2304 96-97 0.597750390394225 2304 98-99 0.5917753631841052 2304 100-101 0.6322018787111361 2202 1 0.1283582088623021 2202 2 0.15804088634031643 2202 3 0.14150409944263487 2202 4 0.20795438415955658 2202 5 0.17875842800192743 2202 6 0.22517958597364185 2202 7 0.21847067737314063 2202 8 0.2234107380424959 2202 9 0.23327319000063085 2202 10-11 0.2132028652145337 2202 12-13 0.21610949403358148 2202 14-15 0.35498652719712 2202 16-17 0.3256699884666219 2202 18-19 0.38627867503134183 2202 20-21 0.3458607236828115 2202 22-23 0.35106541169821526 2202 24-25 0.3952317062183255 2202 26-27 0.4045732266705073 2202 28-29 0.4050971235050298 2202 30-31 0.42136589283311565 2202 32-33 0.43001960096415104 2202 34-35 0.42623282495359405 2202 36-37 0.4095826509999796 2202 38-39 0.4559051629018285 2202 40-41 0.43719562057933814 2202 42-43 0.4836875849513973 2202 44-45 0.46800354600597416 2202 46-47 0.45234942236005793 2202 48-49 0.4676659030174335 2202 50-51 0.4920537002023835 2202 52-53 0.4572809791764385 2202 54-55 0.47178289346554436 2202 56-57 0.4699966743976134 2202 58-59 0.44044482494459203 2202 60-61 0.4547163525258 2202 62-63 0.45710907447546134 2202 64-65 0.4842787301217939 2202 66-67 0.4643680226853206 2202 68-69 0.48311496083105254 2202 70-71 0.4724105305666271 2202 72-73 0.4907276683091837 2202 74-75 0.5199096952462483 2202 76-77 0.48535076184492 2202 78-79 0.5354716359419811 2202 80-81 0.5105401201290576 2202 82-83 0.5627436242137165 2202 84-85 0.5417277790486779 2202 86-87 0.5152233170823166 2202 88-89 0.5456329295614779 2202 90-91 0.4810650190644452 2202 92-93 0.5599119260242986 2202 94-95 0.5895618749037936 2202 96-97 0.6338655063306007 2202 98-99 0.6098383262440024 2202 100-101 0.7096144556198993 1102 1 0.128653718920539 1102 2 0.13583742411608313 1102 3 0.1507774932460677 1102 4 0.19181690790931327 1102 5 0.2068234646460212 1102 6 0.16868811362372327 1102 7 0.17678050699370118 1102 8 0.19129793757779368 1102 9 0.18569109475484424 1102 10-11 0.17667638585312062 1102 12-13 0.18132061900271168 1102 14-15 0.3260273314976203 1102 16-17 0.30443562991234074 1102 18-19 0.3253602115647851 1102 20-21 0.3082534429903703 1102 22-23 0.30976782799936586 1102 24-25 0.29566206705990083 1102 26-27 0.32315184569359445 1102 28-29 0.33905046041281395 1102 30-31 0.35635793662981996 1102 32-33 0.38017879106048014 1102 34-35 0.36220193145343416 1102 36-37 0.3951979189300445 1102 38-39 0.42619279505007057 1102 40-41 0.4133209264656159 1102 42-43 0.39548810266822443 1102 44-45 0.3732443229132656 1102 46-47 0.3957808004486125 1102 48-49 0.37363631692112165 1102 50-51 0.3746347677306332 1102 52-53 0.3759941707114791 1102 54-55 0.41291583247732433 1102 56-57 0.41236039829882287 1102 58-59 0.43653111482489493 1102 60-61 0.4478571398281659 1102 62-63 0.4035355015973181 1102 64-65 0.37435502553167765 1102 66-67 0.41277995149086166 1102 68-69 0.4090263373386449 1102 70-71 0.3635556003240836 1102 72-73 0.4408952183381345 1102 74-75 0.45022881969694595 1102 76-77 0.4672857728371085 1102 78-79 0.46294149726472966 1102 80-81 0.4640787510829085 1102 82-83 0.4885408858723892 1102 84-85 0.4692722312511748 1102 86-87 0.5588324870016237 1102 88-89 0.5479980476494717 1102 90-91 0.46813596468342666 1102 92-93 0.46805583987072197 1102 94-95 0.5160931598704792 1102 96-97 0.5030604115683488 1102 98-99 0.4882253841910469 1102 100-101 0.596652121868182 2305 1 0.10072941631077015 2305 2 0.08614328471124111 2305 3 0.11563234707696779 2305 4 0.14409033610727562 2305 5 0.12693996006124308 2305 6 0.17882407575477544 2305 7 0.12709622770496765 2305 8 0.1602326310378217 2305 9 0.16577142210904583 2305 10-11 0.15198873339391383 2305 12-13 0.12302937114086632 2305 14-15 0.29283910895662046 2305 16-17 0.2930463707018305 2305 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-1.2047876131291844 1213 56-57 -1.1057740789695103 1213 58-59 -1.0279270312379865 1213 60-61 -1.0626884540642862 1213 62-63 -1.0035756984091222 1213 64-65 -0.9488261153171607 1213 66-67 -1.1337015715081407 1213 68-69 -1.1590798361562449 1213 70-71 -1.1286147923304028 1213 72-73 -1.1438042233298304 1213 74-75 -1.14860281709921 1213 76-77 -1.1489221829267606 1213 78-79 -1.172162372139674 1213 80-81 -1.1487294390457023 1213 82-83 -1.221315412389032 1213 84-85 -1.138757503522136 1213 86-87 -1.2417583651284545 1213 88-89 -1.3072889431729138 1213 90-91 -1.4205050771549068 1213 92-93 -1.2773996090805824 1213 94-95 -1.2355895536129182 1213 96-97 -1.3724154257139745 1213 98-99 -1.4230654352567669 1213 100-101 -1.3056727113946707 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10102.0 3 773.0 4 564.0 5 449.0 6 359.0 7 775.0 8 1191.0 9 2407.0 10 6658.0 11 11550.0 12 10945.0 13 10168.0 14 9984.0 15 10079.0 16 10942.0 17 11729.0 18 13438.0 19 15160.0 20 16745.0 21 18782.0 22 21551.0 23 24157.0 24 27625.0 25 31424.0 26 36197.0 27 42953.0 28 51067.0 29 63932.0 30 80002.0 31 104922.0 32 141124.0 33 194916.0 34 277293.0 35 421965.0 36 869975.0 37 4156454.0 38 4698139.0 39 1790.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 26.240592337690483 16.20416102798626 19.126994916783914 38.42825171753935 2 25.021531433699025 27.340010235384888 29.108374400233387 18.530083930682693 3 22.324466120185917 28.531804949571253 29.739344323155432 19.4043846070874 4 24.812564167133104 31.24329912344306 22.279767778722892 21.664368930700945 5 26.983320325651956 33.668206523360986 21.994576095287147 17.35389705569991 6 19.557542781921896 38.13037297060114 22.31319877139096 19.998885476086002 7 18.55607016312158 17.77517524325629 41.936642569576236 21.732112024045897 8 22.226995159831738 22.77656552963007 28.238246324240812 26.75819298629738 9 24.276632701706298 23.598743532837982 27.40682161409973 24.71780215135599 10-11 24.107940504012685 31.961665425127183 22.178069660673266 21.752324410186866 12-13 23.065117042994316 26.24073365028639 27.573420127513536 23.120729179205753 14-15 23.07242457140838 27.613420773287494 27.518769502759326 21.795385152544796 16-17 23.848680066775525 27.797879823543646 26.698626768726676 21.654813340954153 18-19 23.67958641314548 28.04989844322925 26.75747902544125 21.513036118184026 20-21 23.819078453577472 28.111100246972075 26.656936944737065 21.412884354713388 22-23 23.98289651394558 27.93023501820916 26.66898569910488 21.41788276874038 24-25 23.382179639051195 28.390240172695435 26.735681168311903 21.491899019941467 26-27 23.752361492566436 28.5047288220741 26.476097703725376 21.26681198163409 28-29 23.78014143743135 27.92773033750474 26.785320360320377 21.506807864743532 30-31 23.554241296267854 27.95827783948514 26.85779408273577 21.629686781511236 32-33 23.69241925304644 28.154288839224655 26.664222897383937 21.489069010344963 34-35 23.817032256898035 28.00078886970498 26.649353603867603 21.532825269529386 36-37 23.371210297616717 27.992952438438834 26.990873164649965 21.644964099294484 38-39 23.70811764602103 27.962998486913758 26.741206692092064 21.587677174973148 40-41 24.027061014153674 27.707239530275725 26.737609528469225 21.52808992710138 42-43 23.766402591820302 27.703516221986153 26.95891478322426 21.571166402969293 44-45 24.056838950820403 27.81002460667219 26.671607807798768 21.46152863470864 46-47 23.988593412008687 27.696059233164323 26.740256141397108 21.575091213429882 48-49 23.60398287875312 27.732935866515685 26.9303022552912 21.73277899943999 50-51 24.123506431509515 27.8289398423257 26.658122826896985 21.389430899267804 52-53 24.12667480501746 27.6468786557855 26.790579054482777 21.43586748471426 54-55 23.86069019425325 27.77263715460497 26.751498795119826 21.61517385602195 56-57 24.092219376708854 27.810251551980546 26.717255910801622 21.380273160508978 58-59 24.314595306148302 27.89724663117285 26.544323384590555 21.24383467808829 60-61 24.087948336505956 28.018986162943065 26.56897018099203 21.324095319558946 62-63 24.381738914442145 28.07706524923515 26.274546431010283 21.26664940531242 64-65 24.59690159655396 27.95232430278419 26.3041532499442 21.14662085071765 66-67 24.334965993951528 28.040688361270377 26.460719689506234 21.163625955271865 68-69 24.763244660091512 28.045046027320446 26.136475869907688 21.05523344268035 70-71 24.932148899315578 27.961843612683147 26.064767767332807 21.041239720668468 72-73 24.788769851948715 28.047595102154272 26.189528775690363 20.97410627020665 74-75 25.230285992694146 28.134405445711103 25.831699117369784 20.803609444224968 76-77 25.482577499669883 27.925596734815432 25.81499675504373 20.776829010470955 78-79 25.37441620001139 28.098779576837003 25.86534225887337 20.66146196427824 80-81 25.876216837715376 28.153516848636972 25.419747607988114 20.55051870565953 82-83 26.103553641943435 28.02126519093423 25.467631978710237 20.407549188412098 84-85 26.050349009591798 28.085934461319372 25.507006450972614 20.356710078116215 86-87 26.434972377220227 28.175252915023652 25.16008733739798 20.22968737035814 88-89 26.80001527009641 28.03429540791342 25.16448702129564 20.001202300694526 90-91 26.777722753071174 28.103829397740444 25.14110150724199 19.977346341946387 92-93 27.21349339728942 28.184211700577666 24.834638814261808 19.767656087871107 94-95 27.55055482684326 28.04465028281569 24.846072050596398 19.55872283974465 96-97 27.531163189698887 28.16296514438486 24.761969154336864 19.543902511579386 98-99 27.96905575957929 28.231725475751045 24.55679750021541 19.242421264454247 100-101 28.231446565160095 28.152925786260642 24.43875124806758 19.176876400511684 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 8568.0 1 4708.5 2 650.5 3 408.0 4 318.0 5 277.5 6 267.0 7 242.0 8 248.5 9 273.0 10 294.5 11 320.0 12 342.0 13 375.0 14 421.0 15 481.0 16 557.0 17 646.5 18 762.0 19 905.0 20 1091.5 21 1297.5 22 1661.5 23 2307.5 24 3323.5 25 4903.5 26 7355.5 27 10900.0 28 15847.5 29 22773.0 30 30860.5 31 41002.0 32 54057.5 33 68794.5 34 87298.5 35 113754.0 36 151717.0 37 197141.5 38 251268.0 39 318015.0 40 395283.0 41 477155.5 42 561726.5 43 645033.5 44 714295.5 45 763216.5 46 790803.5 47 794055.0 48 771618.5 49 724845.0 50 656572.5 51 573135.5 52 482505.0 53 398828.0 54 317857.5 55 242820.5 56 183685.5 57 137738.5 58 102991.5 59 75490.5 60 53462.5 61 37130.0 62 27249.5 63 19720.5 64 13675.0 65 10590.5 66 8183.5 67 5849.0 68 4696.5 69 4017.0 70 3591.0 71 2699.5 72 1497.5 73 1051.0 74 1003.0 75 796.0 76 588.0 77 269.5 78 90.0 79 60.5 80 42.5 81 38.0 82 36.0 83 26.5 84 27.5 85 23.0 86 13.5 87 11.0 88 9.0 89 6.5 90 4.0 91 2.0 92 2.5 93 2.5 94 1.5 95 2.0 96 3.0 97 3.0 98 1.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1272320837678859 2 0.12691652365657732 3 0.12525106751355988 4 0.1331225391789792 5 0.1316411597675584 6 0.11645921219015723 7 0.11755490702108976 8 0.11413633914858026 9 0.1209471782176569 10-11 0.08592877142105308 12-13 0.09286232831119416 14-15 0.14435560258569954 16-17 0.14627087715016962 18-19 0.14575370918996947 20-21 0.17668298287753303 22-23 0.14937388491137057 24-25 0.14612624543248653 26-27 0.1343979279621847 28-29 0.14493851223575566 30-31 0.10301722800427689 32-33 0.1167616239634946 34-35 0.12946291844366456 36-37 0.1325834573221604 38-39 0.13452064578324913 40-41 0.16512997658018042 42-43 0.14687131791752064 44-45 0.13000638307980708 46-47 0.12266084493323538 48-49 0.1444169614962318 50-51 0.12027661298112616 52-53 0.11987778006266672 54-55 0.10634375751098807 56-57 0.09990107190510478 58-59 0.11037153170949607 60-61 0.11060820179297748 62-63 0.09604422610022224 64-65 0.11251909357812384 66-67 0.10393761166226023 68-69 0.1105205462065029 70-71 0.09395364036280297 72-73 0.09955044955920636 74-75 0.1310713984554735 76-77 0.16042725436581795 78-79 0.12810863963263192 80-81 0.11774336653201016 82-83 0.10856582662811924 84-85 0.10740000732800703 86-87 0.11140586762989636 88-89 0.11803701274670007 90-91 0.1351999765784273 92-93 0.11000337824630274 94-95 0.11056437399974017 96-97 0.10859650608338535 98-99 0.1004883643344846 100-101 0.09349344853381131 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.1408286E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.92160140102001 #Duplication Level Percentage of deduplicated Percentage of total 1 69.20037791590484 33.161929272859396 2 15.79767189550672 15.140994712811388 3 5.777259118484461 8.305665259992617 4 2.896131160413241 5.551489722975875 5 1.6125558755462504 3.8638129952400115 6 1.0892494575289884 3.131914699798888 7 0.6856546201754825 2.3000367184772075 8 0.4444428633343634 1.70387309937899 9 0.3692380401277244 1.5925030362985177 >10 1.9656617159624468 16.649282285466278 >50 0.10626592999610264 3.5367175742939514 >100 0.05364150349477921 4.353715691821574 >500 0.0015249777964459533 0.4653096039095085 >1k 3.062389865131503E-4 0.1927506857939965 >5k 1.868674161433698E-5 0.05000464088176198 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 2.2790452483396716E-4 8.765558647460275E-6 0.0 2 2.454356421288877E-4 8.765558647460275E-6 0.0 3 3.243256699560302E-4 8.765558647460275E-6 0.0 4 6.398857812646001E-4 8.765558647460275E-6 0.0 5 7.01244691796822E-4 8.765558647460275E-6 0.0 6 9.291492166307893E-4 8.765558647460275E-6 0.0 7 0.0010518670376952332 8.765558647460275E-6 0.0 8 0.0012008815347020578 1.753111729492055E-5 0.0 9 0.0013148337971190414 1.753111729492055E-5 0.0 10-11 0.0017925567434056264 2.191389661865069E-5 0.0 12-13 0.002318490262253243 3.50622345898411E-5 0.0 14-15 0.0026822609461228447 3.50622345898411E-5 0.0 16-17 0.003094242202553477 3.50622345898411E-5 0.0 18-19 0.003589496266134983 3.50622345898411E-5 0.0 20-21 0.004176788695514822 3.9445013913571236E-5 0.0 22-23 0.005526684727223704 4.382779323730138E-5 0.0 24-25 0.007086954166471633 5.2593351884761654E-5 0.0 26-27 0.009992736858104714 6.135891053222193E-5 0.0 28-29 0.017246236638878094 6.574168985595206E-5 0.0 30-31 0.03030253624427017 7.01244691796822E-5 0.0 32-33 0.05262403134002777 7.01244691796822E-5 0.0 34-35 0.08465776541717135 7.889002782714248E-5 0.0 36-37 0.13125547518707018 8.765558647460276E-5 0.0 38-39 0.19603295359180162 9.642114512206304E-5 0.0 40-41 0.28195734223353097 1.0518670376952331E-4 0.0 42-43 0.3965494904317791 1.0518670376952331E-4 0.0 44-45 0.5313900791056606 1.1833504174071372E-4 8.765558647460275E-6 46-47 0.6875222097342231 1.2271782106444386E-4 1.3148337971190412E-5 48-49 0.8713491229094361 1.2271782106444386E-4 1.753111729492055E-5 50-51 1.0918379851276518 1.3148337971190412E-4 1.753111729492055E-5 52-53 1.3558872910444215 1.4463171768309453E-4 1.753111729492055E-5 54-55 1.6624977669739347 1.5778005565428497E-4 1.753111729492055E-5 56-57 2.0122830020215132 1.5778005565428497E-4 1.753111729492055E-5 58-59 2.408977124170975 1.621628349780151E-4 1.753111729492055E-5 60-61 2.8613150126145155 1.7092839362547538E-4 1.753111729492055E-5 62-63 3.3792499591963248 1.7969395227293567E-4 1.753111729492055E-5 64-65 3.961129656111356 1.840767315966658E-4 1.753111729492055E-5 66-67 4.60141865307374 1.840767315966658E-4 1.753111729492055E-5 68-69 5.308939484862144 1.840767315966658E-4 1.753111729492055E-5 70-71 6.092089556660834 1.9284229024412608E-4 1.753111729492055E-5 72-73 6.9476431428875465 1.9284229024412608E-4 1.753111729492055E-5 74-75 7.880745626468341 1.9284229024412608E-4 1.753111729492055E-5 76-77 8.878226755535406 1.9284229024412608E-4 1.753111729492055E-5 78-79 9.951551880799622 2.059906282153165E-4 1.753111729492055E-5 80-81 11.10206213273405 2.191389661865069E-4 1.753111729492055E-5 82-83 12.317748695991668 2.191389661865069E-4 1.753111729492055E-5 84-85 13.614376427799934 2.191389661865069E-4 1.753111729492055E-5 86-87 14.977425180259331 2.191389661865069E-4 1.753111729492055E-5 88-89 16.395990598412418 2.191389661865069E-4 1.753111729492055E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGCGCCC 485 0.0 25.95504 44-45 GGGCGCC 1740 0.0 24.436876 42-43 GGGGGCG 1740 0.0 22.120037 40-41 GGGGCGC 1725 0.0 21.069016 42-43 TCGGGGG 3280 0.0 20.35413 38-39 GGGGCCC 705 0.0 17.855595 44-45 GGGGGGC 2090 0.0 17.392628 40-41 CGGGTAG 1220 0.0 16.552767 14-15 CTCGGGG 2620 0.0 16.32253 36-37 GTAGGGG 1890 0.0 15.588584 18-19 GAGCGGC 2745 0.0 15.228427 9 AGGGTAG 2880 0.0 14.43184 28-29 GGGCCCG 400 0.0 13.657004 44-45 CGGGGGG 3035 0.0 13.621051 38-39 GGGTAGA 3300 0.0 13.530693 28-29 GATCGGG 3765 0.0 13.246551 1 GGGGGTC 1440 0.0 13.034208 40-41 CGGGGGT 1460 0.0 12.855659 38-39 GAGGGGC 1815 0.0 12.824314 9 GCGCCCG 910 0.0 12.789166 44-45 >>END_MODULE