FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l03n02 tsrexp_lib#25.3420000000cd24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l03n02 tsrexp_lib#25.3420000000cd24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11408286
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCCC4850.025.9550444-45
GGGCGCC17400.024.43687642-43
GGGGGCG17400.022.12003740-41
GGGGCGC17250.021.06901642-43
TCGGGGG32800.020.3541338-39
GGGGCCC7050.017.85559544-45
GGGGGGC20900.017.39262840-41
CGGGTAG12200.016.55276714-15
CTCGGGG26200.016.3225336-37
GTAGGGG18900.015.58858418-19
GAGCGGC27450.015.2284279
AGGGTAG28800.014.4318428-29
GGGCCCG4000.013.65700444-45
CGGGGGG30350.013.62105138-39
GGGTAGA33000.013.53069328-29
GATCGGG37650.013.2465511
GGGGGTC14400.013.03420840-41
CGGGGGT14600.012.85565938-39
GAGGGGC18150.012.8243149
GCGCCCG9100.012.78916644-45
Produced by FastQC (version 0.11.2)