FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l03n02 tsrexp_lib#22.3420000000ccfc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l03n02 tsrexp_lib#22.3420000000ccfc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7639951
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGCC12300.029.35281842-43
GGGGCGC11550.026.3232442-43
GGCGCCC3850.025.29216844-45
GGGGGCG12600.024.32293140-41
TCGGGGG21650.023.92203738-39
GGGGCCC5000.023.27496144-45
GGGCCCG3800.021.87496644-45
CGGGTAG8500.019.56745114-15
GGGGGGC16600.019.03444940-41
CTCGGGG18150.017.67093736-37
GCGCCCG5550.016.26123444-45
CGGGGGG21200.015.80093838-39
GTAGGGG14750.015.30531418-19
GAGCGGC19350.014.9739419
CGGGGGT8300.013.73924138-39
AGGGTAG22500.013.72256128-29
GGGCGGG10950.013.66512312-13
GGGTAGA25050.013.55821828-29
GATCGGG27800.013.49844551
GGGCCCC3550.013.38175242-43
Produced by FastQC (version 0.11.2)