FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l03n02 tsrexp_lib#2.3420000000ca41.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l03n02 tsrexp_lib#2.3420000000ca41.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17801708
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG269460.1513674979951362Illumina Single End PCR Primer 1 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGCGC55300.036.29636842-43
TCGGGGG86500.035.4017938-39
GGCGCCC20850.034.857744-45
GGGCGCC72250.031.7264242-43
GGGGGCG68600.030.26727340-41
AAGGGGC38250.029.804239
GTAGGGG48050.028.6823118-19
AGGGTAG70950.028.18475728-29
CTCGGGG71950.028.16515736-37
GAGCGGC59650.027.7119359
GATCGGG71700.027.4948561
GAGGGGC30900.026.44049
GGGTAGA82750.025.39977628-29
GGGCCCG12950.025.3100344-45
GGGCGGG41400.025.24329612-13
CGGGTAG38450.025.21181914-15
GGGGGGC81100.024.92841540-41
AGAGCGG68100.024.268538
GGGTAGG43750.023.89752416-17
CGGGGGG84750.023.77084438-39
Produced by FastQC (version 0.11.2)