##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C5B16ACXX l03n02 tsrexp_lib#2.3420000000ca41.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 17801708 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.514111174051386 33.0 33.0 33.0 33.0 33.0 2 32.42920347867744 33.0 33.0 33.0 33.0 33.0 3 32.40381647648641 33.0 33.0 33.0 33.0 33.0 4 36.260681503145655 37.0 37.0 37.0 37.0 37.0 5 36.26663941459999 37.0 37.0 37.0 37.0 37.0 6 36.31840326782127 37.0 37.0 37.0 37.0 37.0 7 36.273320683610805 37.0 37.0 37.0 37.0 37.0 8 36.33674139582561 37.0 37.0 37.0 37.0 37.0 9 36.25301431750257 37.0 37.0 37.0 37.0 37.0 10-11 36.25116205141664 37.0 37.0 37.0 37.0 37.0 12-13 36.25080694504145 37.0 37.0 37.0 37.0 37.0 14-15 38.60526621939872 40.0 40.0 40.0 37.0 40.0 16-17 38.587538257564944 40.0 40.0 40.0 37.0 40.0 18-19 38.58835329171785 40.0 40.0 40.0 37.0 40.0 20-21 38.57061210081639 40.0 40.0 40.0 37.0 40.0 22-23 38.63215591447742 40.0 40.0 40.0 37.0 40.0 24-25 38.54799157474103 40.0 40.0 40.0 37.0 40.0 26-27 38.54906079798635 40.0 40.0 40.0 37.0 40.0 28-29 38.469742790972646 40.0 40.0 40.0 37.0 40.0 30-31 38.43583969583143 40.0 40.0 40.0 37.0 40.0 32-33 38.40548381649671 40.0 40.0 40.0 37.0 40.0 34-35 38.378321844173605 40.0 40.0 40.0 37.0 40.0 36-37 38.35310417966636 40.0 40.0 40.0 37.0 40.0 38-39 38.32950436553616 40.0 40.0 40.0 37.0 40.0 40-41 38.290782126074646 40.0 38.5 40.0 37.0 40.0 42-43 38.1961819056913 40.0 37.0 40.0 37.0 40.0 44-45 38.16135064118566 40.0 37.0 40.0 37.0 40.0 46-47 38.04076021806447 40.0 37.0 40.0 37.0 40.0 48-49 37.9663575259183 40.0 37.0 40.0 37.0 40.0 50-51 36.58649403192098 38.5 37.0 38.5 35.0 40.0 52-53 36.91433704001886 38.5 37.0 40.0 33.0 40.0 54-55 37.55209042300885 40.0 37.0 40.0 33.0 40.0 56-57 37.53546738324211 40.0 37.0 40.0 33.0 40.0 58-59 37.34495226525455 40.0 37.0 40.0 33.0 40.0 60-61 37.20483677745978 37.0 37.0 40.0 33.0 40.0 62-63 36.992864532998745 37.0 37.0 40.0 33.0 40.0 64-65 36.7312833128147 37.0 37.0 40.0 33.0 40.0 66-67 36.45019376792384 37.0 37.0 40.0 33.0 40.0 68-69 36.13317368198602 37.0 37.0 37.0 33.0 40.0 70-71 35.867281695666506 37.0 37.0 37.0 33.0 40.0 72-73 35.649858625925106 37.0 37.0 37.0 33.0 40.0 74-75 35.448583135955275 37.0 37.0 37.0 33.0 37.0 76-77 35.27600904924404 37.0 37.0 37.0 33.0 37.0 78-79 35.15177560490263 37.0 37.0 37.0 33.0 37.0 80-81 35.05472663072555 37.0 37.0 37.0 33.0 37.0 82-83 34.97368362069527 37.0 37.0 37.0 33.0 37.0 84-85 34.90733198185252 37.0 37.0 37.0 33.0 37.0 86-87 34.850174236090155 37.0 35.0 37.0 33.0 37.0 88-89 34.78672785218137 37.0 33.0 37.0 33.0 37.0 90-91 34.717637543543574 37.0 33.0 37.0 33.0 37.0 92-93 34.678028956547315 37.0 33.0 37.0 33.0 37.0 94-95 34.621231344767594 37.0 33.0 37.0 33.0 37.0 96-97 34.570250646735694 37.0 33.0 37.0 33.0 37.0 98-99 34.51203013778229 37.0 33.0 37.0 33.0 37.0 100-101 32.71685163580933 35.0 30.0 37.0 27.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.1805472798866461 2306 2 0.20364758191181664 2306 3 0.19766167733932605 2306 4 0.2744924692991333 2306 5 0.27972728536349933 2306 6 0.29815522272910044 2306 7 0.25688586566958804 2306 8 0.28031066911403 2306 9 0.2799728020641936 2306 10-11 0.28473869938807894 2306 12-13 0.2763028073706266 2306 14-15 0.49105677904400835 2306 16-17 0.4962667339832123 2306 18-19 0.49422564836280003 2306 20-21 0.5034399085039851 2306 22-23 0.5525493732097218 2306 24-25 0.56308242970821 2306 26-27 0.541333529271256 2306 28-29 0.5574610744794981 2306 30-31 0.5709853446323621 2306 32-33 0.5464360067980181 2306 34-35 0.5850888755957371 2306 36-37 0.5912628317870912 2306 38-39 0.5941596448517004 2306 40-41 0.5741619090800185 2306 42-43 0.6245620832294421 2306 44-45 0.6628448553329136 2306 46-47 0.6080492980728422 2306 48-49 0.6313669899098855 2306 50-51 0.2813127735221812 2306 52-53 0.46863363985605844 2306 54-55 0.6073919022043057 2306 56-57 0.6161506643816637 2306 58-59 0.636701412018347 2306 60-61 0.6651763092714162 2306 62-63 0.6552375995756634 2306 64-65 0.6425396590864594 2306 66-67 0.6420343285185908 2306 68-69 0.676301826794294 2306 70-71 0.6022627610846811 2306 72-73 0.6248662796506324 2306 74-75 0.638908612262675 2306 76-77 0.6349758132297083 2306 78-79 0.6437789851480531 2306 80-81 0.6645962909173946 2306 82-83 0.660333581293358 2306 84-85 0.6829891658820557 2306 86-87 0.7657505028372782 2306 88-89 0.7538451740168455 2306 90-91 0.7472123494776213 2306 92-93 0.7797779332217587 2306 94-95 0.7452274353111292 2306 96-97 0.7215548000383265 2306 98-99 0.751486006752188 2306 100-101 0.6315494587926764 2307 1 0.13562491683588007 2307 2 0.15797667661842496 2307 3 0.14709599850395705 2307 4 0.19120700096100762 2307 5 0.1570477711335272 2307 6 0.18550816477640097 2307 7 0.15907815048467455 2307 8 0.17434852822434976 2307 9 0.1740045380758488 2307 10-11 0.1466999746695734 2307 12-13 0.14842747600718553 2307 14-15 0.3175499045386445 2307 16-17 0.34327053624353 2307 18-19 0.3574914633937496 2307 20-21 0.36828841752420516 2307 22-23 0.39625124414310875 2307 24-25 0.39885270229366654 2307 26-27 0.3411784037637702 2307 28-29 0.3865369825599956 2307 30-31 0.3693049324705626 2307 32-33 0.39890624700552024 2307 34-35 0.42539736291303853 2307 36-37 0.41664352427819296 2307 38-39 0.42499828266600304 2307 40-41 0.4723906481791289 2307 42-43 0.4611790866312049 2307 44-45 0.4516988903489434 2307 46-47 0.4268528934722866 2307 48-49 0.4477546330727975 2307 50-51 0.33568183831022225 2307 52-53 0.3982282409684146 2307 54-55 0.4348329735990504 2307 56-57 0.44235913148659733 2307 58-59 0.40752141914425977 2307 60-61 0.41228887843724493 2307 62-63 0.45544201120956274 2307 64-65 0.4617251291117199 2307 66-67 0.47305233633279187 2307 68-69 0.4613241817107152 2307 70-71 0.4935037350109184 2307 72-73 0.5186964833739225 2307 74-75 0.49336058152917417 2307 76-77 0.4974970147873492 2307 78-79 0.5345117900635188 2307 80-81 0.5508848781114537 2307 82-83 0.5592034762737086 2307 84-85 0.5449351815957684 2307 86-87 0.568071535656685 2307 88-89 0.6030747576830962 2307 90-91 0.5963677420363069 2307 92-93 0.5897021420495179 2307 94-95 0.5997220604130717 2307 96-97 0.5965058699976993 2307 98-99 0.6114558558785106 2307 100-101 0.6407468610641232 2201 1 0.19684752182386234 2201 2 0.1558803923591796 2201 3 0.18345467532837034 2201 4 0.2289644985826058 2201 5 0.21853744271489717 2201 6 0.2901029933908532 2201 7 0.22045828033719772 2201 8 0.2556969523925048 2201 9 0.2465529595820115 2201 10-11 0.22987051018525761 2201 12-13 0.24348631127860187 2201 14-15 0.43379948459770645 2201 16-17 0.4562513558921282 2201 18-19 0.46230718523187875 2201 20-21 0.4851161536598596 2201 22-23 0.5142065618435865 2201 24-25 0.48320411233678584 2201 26-27 0.47412226542422076 2201 28-29 0.5087423211059772 2201 30-31 0.48359648288815293 2201 32-33 0.48667890241295453 2201 34-35 0.4827789143478114 2201 36-37 0.5400596803504314 2201 38-39 0.5427221317375697 2201 40-41 0.5272822226644038 2201 42-43 0.5381513859899698 2201 44-45 0.5582440006824569 2201 46-47 0.5405564159601255 2201 48-49 0.5349379330937509 2201 50-51 0.4845563797911794 2201 52-53 0.5595458484942171 2201 54-55 0.578472786836862 2201 56-57 0.5866079597449669 2201 58-59 0.5485924708760166 2201 60-61 0.572141531271896 2201 62-63 0.5907084708012462 2201 64-65 0.5722502246191326 2201 66-67 0.5966247429111249 2201 68-69 0.5869185216971928 2201 70-71 0.5745467512106472 2201 72-73 0.5852217425656008 2201 74-75 0.6151248814284003 2201 76-77 0.6326980484619682 2201 78-79 0.6252600503385608 2201 80-81 0.5942722922677035 2201 82-83 0.6265269661673472 2201 84-85 0.6743316364048866 2201 86-87 0.6606752352927288 2201 88-89 0.6652949640983721 2201 90-91 0.655791957328411 2201 92-93 0.7011893870399959 2201 94-95 0.7096799557867186 2201 96-97 0.6910462891699396 2201 98-99 0.7088396298298392 2201 100-101 0.7049931115785313 1101 1 0.1967737267735572 1101 2 0.19588155170551147 1101 3 0.19726759159493668 1101 4 0.24008598139906212 1101 5 0.22327004444392173 1101 6 0.2600531582949728 1101 7 0.22872190413118432 1101 8 0.23184669837730354 1101 9 0.2580913461523764 1101 10-11 0.2433083263517588 1101 12-13 0.24441598750965454 1101 14-15 0.39802010757995987 1101 16-17 0.4030448355689771 1101 18-19 0.3960289490648279 1101 20-21 0.4074617026937446 1101 22-23 0.39094107852526605 1101 24-25 0.4439636724617202 1101 26-27 0.4692289801683458 1101 28-29 0.4476660617806729 1101 30-31 0.401683883305175 1101 32-33 0.45726964522778957 1101 34-35 0.5070110222023345 1101 36-37 0.5097634763464072 1101 38-39 0.4867662028353408 1101 40-41 0.4940407672675704 1101 42-43 0.5145030488570512 1101 44-45 0.5209555945715678 1101 46-47 0.5333417740409203 1101 48-49 0.5374615581931792 1101 50-51 0.45978905470199294 1101 52-53 0.5512716155875594 1101 54-55 0.555837940268809 1101 56-57 0.5471618726111132 1101 58-59 0.5228939813113271 1101 60-61 0.5397461213615173 1101 62-63 0.49807952291546087 1101 64-65 0.5390344248379293 1101 66-67 0.519186313947273 1101 68-69 0.5181723699907081 1101 70-71 0.517072434071693 1101 72-73 0.49904540075405635 1101 74-75 0.5514287415712431 1101 76-77 0.5364410262234927 1101 78-79 0.5512331229575054 1101 80-81 0.5607480430821496 1101 82-83 0.554585854398745 1101 84-85 0.5515112983296504 1101 86-87 0.5772161036376673 1101 88-89 0.5868085805780865 1101 90-91 0.6034447321640926 1101 92-93 0.6560378706692873 1101 94-95 0.6014886231739709 1101 96-97 0.5812954521496252 1101 98-99 0.6067462089366842 1101 100-101 0.6135324512112703 2304 1 0.1445799617793 2304 2 0.14222687724765848 2304 3 0.16637539596355566 2304 4 0.20603593199204084 2304 5 0.19251099142027073 2304 6 0.22179274971669116 2304 7 0.19288016250096973 2304 8 0.2145473345543394 2304 9 0.19865434840424 2304 10-11 0.21137836770669338 2304 12-13 0.19961657749429662 2304 14-15 0.3372643975895926 2304 16-17 0.3726732199380507 2304 18-19 0.36601794457450865 2304 20-21 0.3937497623259105 2304 22-23 0.4113799427475655 2304 24-25 0.3937412783103511 2304 26-27 0.41886446415025347 2304 28-29 0.40997304049150785 2304 30-31 0.3761959024758781 2304 32-33 0.3734703534589343 2304 34-35 0.4204616598035713 2304 36-37 0.41585943168003325 2304 38-39 0.44329790240772127 2304 40-41 0.5030644161761941 2304 42-43 0.49356841741042956 2304 44-45 0.49041082301865657 2304 46-47 0.464369433202954 2304 48-49 0.5123949243449673 2304 50-51 0.3896920820204386 2304 52-53 0.44084873046754325 2304 54-55 0.48230976334503595 2304 56-57 0.5022509949968992 2304 58-59 0.4740584742528142 2304 60-61 0.5050294245183338 2304 62-63 0.4974003002968459 2304 64-65 0.5008575333151271 2304 66-67 0.4812101430740583 2304 68-69 0.49502151033140507 2304 70-71 0.4996625460899793 2304 72-73 0.49138304247389897 2304 74-75 0.4968253973985952 2304 76-77 0.5062834750419398 2304 78-79 0.5142763828209667 2304 80-81 0.49092482476802246 2304 82-83 0.5526989289805186 2304 84-85 0.47722093935611554 2304 86-87 0.5496514031634732 2304 88-89 0.5414024330801865 2304 90-91 0.5616046224742632 2304 92-93 0.5605026723206521 2304 94-95 0.6183306837954561 2304 96-97 0.5944103275566093 2304 98-99 0.5612890282618537 2304 100-101 0.5927229717662499 2202 1 0.1523468880100296 2202 2 0.15387296175318 2202 3 0.15691242196212585 2202 4 0.218780107329394 2202 5 0.19297385630452624 2202 6 0.24373326360403524 2202 7 0.19507634956594444 2202 8 0.20268341539355816 2202 9 0.21679304531182453 2202 10-11 0.21995880888122343 2202 12-13 0.20937421432198278 2202 14-15 0.37095206521303936 2202 16-17 0.3705085467906599 2202 18-19 0.3798344681211603 2202 20-21 0.35702851352159115 2202 22-23 0.37010619270717626 2202 24-25 0.3681425681594206 2202 26-27 0.41822560312400725 2202 28-29 0.39578478558950536 2202 30-31 0.41756357311886205 2202 32-33 0.4508948799083754 2202 34-35 0.46288138865133277 2202 36-37 0.4349168231555751 2202 38-39 0.4595796574530837 2202 40-41 0.45424495577764645 2202 42-43 0.48374992137178197 2202 44-45 0.45680477452427937 2202 46-47 0.44333826152289646 2202 48-49 0.4794010787330123 2202 50-51 0.5175264968085784 2202 52-53 0.491299196114646 2202 54-55 0.49199010478879046 2202 56-57 0.5058561099296099 2202 58-59 0.47931908125304545 2202 60-61 0.4881523024601364 2202 62-63 0.4659188688674689 2202 64-65 0.521280404556073 2202 66-67 0.5058564626119875 2202 68-69 0.47389191335987135 2202 70-71 0.47580389894916664 2202 72-73 0.5074579572215896 2202 74-75 0.4875800663060659 2202 76-77 0.462009116090762 2202 78-79 0.4797149756961616 2202 80-81 0.5018975474077081 2202 82-83 0.5399995736771217 2202 84-85 0.5292981297170627 2202 86-87 0.5499193889413192 2202 88-89 0.5438104350453088 2202 90-91 0.4774436659868684 2202 92-93 0.5316675214533859 2202 94-95 0.5537734365401761 2202 96-97 0.5501554403121602 2202 98-99 0.5707915776294854 2202 100-101 0.7102676698117207 1102 1 0.13498623107798835 1102 2 0.15810155937718662 1102 3 0.15092450510862676 1102 4 0.18753707173483036 1102 5 0.1787343954598697 1102 6 0.18415409835640162 1102 7 0.18047928635845523 1102 8 0.19423849543804295 1102 9 0.19397908603781389 1102 10-11 0.18569455155679293 1102 12-13 0.20014616959659293 1102 14-15 0.34720767279757325 1102 16-17 0.33314107121316283 1102 18-19 0.3432131285757123 1102 20-21 0.33762112293209867 1102 22-23 0.34367433039596307 1102 24-25 0.34995280151024843 1102 26-27 0.35604454743076275 1102 28-29 0.3861396847627816 1102 30-31 0.38206610587874934 1102 32-33 0.4104617897956615 1102 34-35 0.41163939546002126 1102 36-37 0.39631922714691825 1102 38-39 0.41820593719851473 1102 40-41 0.4411260055097088 1102 42-43 0.41844150277588454 1102 44-45 0.3836721452537617 1102 46-47 0.4292986084097379 1102 48-49 0.434578718355759 1102 50-51 0.4442014614433347 1102 52-53 0.42887364819444684 1102 54-55 0.45315192763151657 1102 56-57 0.4040944284963075 1102 58-59 0.4117259293086448 1102 60-61 0.4434146829239438 1102 62-63 0.42561163331807705 1102 64-65 0.4164825328282973 1102 66-67 0.43244104463263966 1102 68-69 0.42766266347786086 1102 70-71 0.3905739275910918 1102 72-73 0.45199548511457266 1102 74-75 0.4712267782150974 1102 76-77 0.4690612327435204 1102 78-79 0.43828559853614735 1102 80-81 0.4826694781803482 1102 82-83 0.5379822642604211 1102 84-85 0.5354017308717403 1102 86-87 0.5362189519904916 1102 88-89 0.5396416120189187 1102 90-91 0.5051819232646082 1102 92-93 0.5408239319470596 1102 94-95 0.562275168007865 1102 96-97 0.5858015066526221 1102 98-99 0.5826840803309992 1102 100-101 0.6530810587343154 2305 1 0.14038804260452764 2305 2 0.15089401480035036 2305 3 0.1548091588314051 2305 4 0.17330966406898085 2305 5 0.19177269538420916 2305 6 0.23247366623049004 2305 7 0.16472868211623393 2305 8 0.2160524517434368 2305 9 0.19273017195595799 2305 10-11 0.20593929269825395 2305 12-13 0.2092680390383066 2305 14-15 0.3487736457814208 2305 16-17 0.3544380249829686 2305 18-19 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-1.1791206860792656 1213 56-57 -1.1060910705834175 1213 58-59 -1.044093379922714 1213 60-61 -1.0801259913007328 1213 62-63 -1.059112151581317 1213 64-65 -0.991210964081553 1213 66-67 -1.1414804238131921 1213 68-69 -1.142338240467545 1213 70-71 -1.1444789169581 1213 72-73 -1.1511790769421566 1213 74-75 -1.1439760659033595 1213 76-77 -1.159231569429359 1213 78-79 -1.1960097368560199 1213 80-81 -1.211904629416317 1213 82-83 -1.1785947081208121 1213 84-85 -1.1389171932480906 1213 86-87 -1.2269620245245463 1213 88-89 -1.319547398985037 1213 90-91 -1.4246963505669044 1213 92-93 -1.3053010472139945 1213 94-95 -1.2294038519014308 1213 96-97 -1.3723738873444518 1213 98-99 -1.3789779864541316 1213 100-101 -1.2781321313371166 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 16194.0 3 1364.0 4 874.0 5 756.0 6 1225.0 7 6722.0 8 10932.0 9 15173.0 10 29927.0 11 46908.0 12 39370.0 13 29879.0 14 25884.0 15 23217.0 16 22562.0 17 23194.0 18 24972.0 19 27506.0 20 29579.0 21 32999.0 22 37376.0 23 42414.0 24 47862.0 25 52221.0 26 57230.0 27 66214.0 28 79787.0 29 98368.0 30 124002.0 31 161628.0 32 216433.0 33 300684.0 34 426905.0 35 648266.0 36 1358959.0 37 6590913.0 38 7079510.0 39 3699.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 24.030563127995755 14.530664796970012 21.244726361401 40.19404571363323 2 25.222898179471514 25.18549888238031 29.934799002542917 19.65680393560526 3 23.36969828417061 26.30940455934217 30.96469835982273 19.35619879666449 4 25.14401107166063 29.451920060432197 22.617679567272358 22.786389300634816 5 28.627762146616263 32.607186051735106 21.909880245971276 16.855171555677355 6 20.086451893678017 37.120963079742275 22.21648990823717 20.57609511834254 7 18.752533371038275 16.517958806114432 42.945528677099304 21.78397914574799 8 22.261854473276156 22.317352872579264 28.50377091601548 26.917021738129105 9 24.88289897590036 22.81545283562211 27.105518034232645 25.196130154244884 10-11 24.625954565433336 31.12177154862635 21.98147606468773 22.270797821252582 12-13 23.294067240045344 25.07017853608507 27.730550070172917 23.905204153696673 14-15 23.25410477216613 26.53120535967444 27.95602497562925 22.258664892530174 16-17 24.382076982856184 26.532672176630907 27.02873379617603 22.056517044336882 18-19 24.172964208826127 26.76535703199795 27.010511460610186 22.051167298565737 20-21 24.099128428610932 27.322857563802817 26.677268719898585 21.900745287687666 22-23 24.544794683153174 26.86993763688777 26.709391857753378 21.875875822205675 24-25 23.60254115008856 27.7425330966529 26.78727973480765 21.86764601845089 26-27 24.01472568227504 27.859432117884207 26.40063722411495 21.725204975725802 28-29 24.25893126328503 27.114861273080155 26.709931318249335 21.916276145385478 30-31 24.059128942472864 27.02477879773861 26.93508520202723 21.9810070577613 32-33 23.788605198307692 27.41521378104512 26.93163222470238 21.864548795944806 34-35 24.249824726323315 27.09106108748879 26.793897393574678 21.865216792613214 36-37 23.500012726766727 26.998799183855652 27.33989903244229 22.161289056935335 38-39 23.8732352474201 27.002521923918703 27.120600784070408 22.003642044590787 40-41 24.662795944804245 26.593207974478183 26.75877813168473 21.985217949032844 42-43 24.271031772297107 26.704886029256535 27.106490884678085 21.917591313768273 44-45 24.61582478966118 26.808507754013007 26.825562614216448 21.750104842109373 46-47 24.37055848912383 26.704324580999224 26.76326935978124 22.161847570095706 48-49 23.841276977063945 26.67667471835794 26.87876914072234 22.603279163855774 50-51 24.499764478259586 26.90845305102596 26.61755065826569 21.974231812448767 52-53 24.217088545246206 26.98747768562764 27.07306667383588 21.72236709529028 54-55 23.783584145943696 27.083822586631573 26.928597136462308 22.203996130962423 56-57 24.1192189204106 26.94191500075773 27.41907521845593 21.51979086037574 58-59 24.400739771169885 27.43270098219523 26.74359217554007 21.422967071094813 60-61 23.894523544453236 27.7152459296295 26.583528611987745 21.806701913929523 62-63 24.543365576627206 27.760450669689064 26.245165523169373 21.451018230514354 64-65 24.52574935729525 27.686309578977774 26.434185413909056 21.35375564981792 66-67 24.37213151919723 27.79812272166753 26.515754398307944 21.3139913608273 68-69 24.850974903496073 27.75097698994584 26.274771599830633 21.12327650672745 70-71 24.945545382296775 27.72617207737914 26.172634042590037 21.15564849773404 72-73 24.857119384179992 27.71129871951212 26.169398187453474 21.262183708854415 74-75 25.21950752363586 27.758335779032368 25.9645628404965 21.057593856835272 76-77 25.50252176625593 27.652372794228196 26.003164220785592 20.841941218730277 78-79 25.431470543658186 27.64059668975742 26.051159838686555 20.876772927897836 80-81 25.866878747197315 27.75009176723661 25.55750371406676 20.825525771499315 82-83 26.143453351733836 27.714616767817606 25.541398195156113 20.60053168529245 84-85 26.097850574736235 27.712393240343662 25.630575899777412 20.559180285142688 86-87 26.444787240300645 27.80658811418825 25.353777471237372 20.394847174273732 88-89 26.755280520925005 27.68193354084665 25.274200362833337 20.288585575395004 90-91 26.810944487777537 27.72373798041089 25.294368764352093 20.170948767459475 92-93 27.332288275026173 27.85363454887629 24.80105687585679 20.013020300240743 94-95 27.589631438470786 27.760861055463813 24.854127657592954 19.795379848472447 96-97 27.554414187666502 27.94149292963177 24.88107983550018 19.62301304720154 98-99 28.101310581144425 27.97064553307898 24.585441496202947 19.342602389573653 100-101 28.35709033531051 27.920340594343113 24.43043332090112 19.29213574944526 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 13824.0 1 7610.0 2 1109.5 3 732.0 4 585.5 5 530.0 6 529.0 7 522.0 8 531.5 9 564.5 10 617.0 11 674.5 12 719.5 13 780.0 14 881.0 15 978.5 16 1066.0 17 1205.5 18 1386.5 19 1635.0 20 1961.0 21 2349.0 22 3005.0 23 4010.0 24 5522.0 25 7815.0 26 11466.5 27 16797.5 28 24774.0 29 35626.5 30 49913.5 31 67579.5 32 88368.0 33 112751.0 34 140807.5 35 175866.5 36 224669.5 37 285188.0 38 354719.0 39 441420.5 40 549748.0 41 670597.0 42 796858.5 43 923217.5 44 1039018.0 45 1128655.0 46 1183245.5 47 1198859.0 48 1176513.0 49 1119034.5 50 1024426.0 51 909621.0 52 784996.0 53 660894.0 54 541793.5 55 439294.0 56 356979.0 57 292439.5 58 241277.5 59 194012.0 60 153908.0 61 111328.0 62 71702.0 63 46340.5 64 30074.5 65 19594.0 66 13848.5 67 9107.0 68 6450.5 69 5212.0 70 4875.0 71 3912.0 72 2299.0 73 1737.0 74 1647.5 75 1354.5 76 983.5 77 475.5 78 234.5 79 176.0 80 131.0 81 102.0 82 87.5 83 82.0 84 69.0 85 50.5 86 44.0 87 44.5 88 34.0 89 27.0 90 26.0 91 23.5 92 19.5 93 15.5 94 10.0 95 4.0 96 5.5 97 4.0 98 1.5 99 2.0 100 4.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1315772621368691 2 0.13088069976206776 3 0.12897077066987056 4 0.1372284052743703 5 0.13585775027879346 6 0.12029744561589259 7 0.1210277126217327 8 0.11805047021330763 9 0.12463972558138803 10-11 0.08875833712135936 12-13 0.09587844042830047 14-15 0.1477386327199615 16-17 0.14997718196478677 18-19 0.1493424114135565 20-21 0.18084781527705093 22-23 0.1535723426089227 24-25 0.14990415526420275 26-27 0.13820583957449478 28-29 0.14905873076898013 30-31 0.1064195637856772 32-33 0.1205502303486834 34-35 0.1336725666997796 36-37 0.13602908215324058 38-39 0.13821988317076092 40-41 0.16901468106318787 42-43 0.15039848985277143 44-45 0.13360796615695528 46-47 0.1255806465312205 48-49 0.14803916568005723 50-51 0.12376902261288636 52-53 0.12340108039071307 54-55 0.10958499038406877 56-57 0.10320919767923392 58-59 0.11382896517570112 60-61 0.11392165291105776 62-63 0.09884725667896586 64-65 0.11574170298715156 66-67 0.10730711906969825 68-69 0.11407332375073223 70-71 0.09647388890998548 72-73 0.10296764782345605 74-75 0.13425958902370494 76-77 0.1642763716829868 78-79 0.13214743214527505 80-81 0.12172708371578726 82-83 0.11205947204616547 84-85 0.11104833311500223 86-87 0.11501143598131144 88-89 0.12158383903387247 90-91 0.13867208697053113 92-93 0.1137194251248251 94-95 0.11415477660907593 96-97 0.11193027096051682 98-99 0.10365297532124444 100-101 0.0969963106910865 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.7801708E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.70652983463586 #Duplication Level Percentage of deduplicated Percentage of total 1 67.39013660447817 27.43218606250377 2 15.061572841792284 12.262087284819174 3 6.351674686707118 7.756639054030338 4 3.3611846321472503 5.472886500328864 5 1.8213719912931727 3.7070866651772825 6 1.163918980992833 2.842746163493023 7 0.8720531125742547 2.484877923107367 8 0.6948719302977177 2.262865996953204 9 0.4490053713646391 1.6449705490829791 >10 2.527463188727555 18.485984890837777 >50 0.19070348065710455 5.3187391526425705 >100 0.10997582826037186 7.928307806254338 >500 0.004664003519630962 1.2823054689663698 >1k 0.0013755359589845359 0.9146076564181606 >5k 1.4012977799334304E-5 0.052358824158422905 >10k+ 1.379825104756932E-5 0.15135000122640832 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG 26946 0.1513674979951362 Illumina Single End PCR Primer 1 (100% over 50bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 2.527847327908086E-4 0.0 0.0 2 4.493950805169931E-4 0.0 0.0 3 5.785961661656286E-4 0.0 0.0 4 0.0015167083967448517 0.0 0.0 5 0.0016515269208999496 0.0 0.0 6 0.0021514789479751043 1.1234877012924827E-5 0.0 7 0.00260649146699856 1.1234877012924827E-5 0.0 8 0.003803005868875054 1.1234877012924827E-5 0.0 9 0.004184991687314498 1.1234877012924827E-5 0.0 10-11 0.005041651059550016 1.685231551938724E-5 0.0 12-13 0.005873031958506454 1.685231551938724E-5 0.0 14-15 0.006906640643695537 1.685231551938724E-5 0.0 16-17 0.008159329430636656 1.685231551938724E-5 0.0 18-19 0.010423157148741008 2.8087192532312068E-5 0.0 20-21 0.01321783280570606 5.6174385064624136E-5 0.0 22-23 0.017512364543896576 6.460054282431775E-5 0.0 24-25 0.02312699433110576 7.021798133078017E-5 0.0 26-27 0.031072861098496843 7.302670058401138E-5 0.0 28-29 0.048470068153010935 7.583541983724259E-5 0.0 30-31 0.07830990149933928 8.1452858343705E-5 0.0 32-33 0.11921047126489212 9.268773535662983E-5 0.0 34-35 0.16703453398965987 1.0111389311632344E-4 0.0 36-37 0.22940214500765882 1.1515748938247948E-4 0.0 38-39 0.30853500124819483 1.2920108564863552E-4 0.0 40-41 0.4092275864765336 1.4043596266156034E-4 0.0 42-43 0.5369512857979695 1.5167083967448518E-4 5.617438506462413E-6 44-45 0.686743092292043 1.5447955892771638E-4 5.617438506462413E-6 46-47 0.8595776315396253 1.600969974341788E-4 5.617438506462413E-6 48-49 1.0628558787729807 1.6290571668741E-4 5.617438506462413E-6 50-51 1.2972519266128846 1.7133187444710364E-4 5.617438506462413E-6 52-53 1.5647009826248133 1.7694931295356603E-4 5.617438506462413E-6 54-55 1.864256508420428 1.8537547071325965E-4 5.617438506462413E-6 56-57 2.2015247076291784 1.8537547071325965E-4 1.1234877012924827E-5 58-59 2.580131636806985 1.9661034772618447E-4 1.1234877012924827E-5 60-61 3.0077170123226376 1.9661034772618447E-4 1.1234877012924827E-5 62-63 3.4871260667796595 1.9661034772618447E-4 1.1234877012924827E-5 64-65 4.0223949297449435 1.9661034772618447E-4 1.1234877012924827E-5 66-67 4.613006796875895 1.9941906697941566E-4 1.1234877012924827E-5 68-69 5.265076249986799 2.0222778623264689E-4 1.4043596266156032E-5 70-71 5.990259473978565 2.0222778623264689E-4 1.685231551938724E-5 72-73 6.79631696014787 2.0222778623264689E-4 1.685231551938724E-5 74-75 7.682552146119912 2.0222778623264689E-4 1.685231551938724E-5 76-77 8.646518637425128 2.106539439923405E-4 1.685231551938724E-5 78-79 9.699139543239333 2.1346266324557173E-4 1.9661034772618447E-5 80-81 10.84985496897264 2.1346266324557173E-4 2.8087192532312068E-5 82-83 12.091952075609823 2.1627138249880293E-4 2.8087192532312068E-5 84-85 13.428396870682297 2.1908010175203412E-4 2.8087192532312068E-5 86-87 14.843814424997872 2.2469754025849654E-4 2.8087192532312068E-5 88-89 16.32400385401221 2.3031497876495896E-4 2.8087192532312068E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGGCGC 5530 0.0 36.296368 42-43 TCGGGGG 8650 0.0 35.40179 38-39 GGCGCCC 2085 0.0 34.8577 44-45 GGGCGCC 7225 0.0 31.72642 42-43 GGGGGCG 6860 0.0 30.267273 40-41 AAGGGGC 3825 0.0 29.80423 9 GTAGGGG 4805 0.0 28.68231 18-19 AGGGTAG 7095 0.0 28.184757 28-29 CTCGGGG 7195 0.0 28.165157 36-37 GAGCGGC 5965 0.0 27.711935 9 GATCGGG 7170 0.0 27.494856 1 GAGGGGC 3090 0.0 26.4404 9 GGGTAGA 8275 0.0 25.399776 28-29 GGGCCCG 1295 0.0 25.31003 44-45 GGGCGGG 4140 0.0 25.243296 12-13 CGGGTAG 3845 0.0 25.211819 14-15 GGGGGGC 8110 0.0 24.928415 40-41 AGAGCGG 6810 0.0 24.26853 8 GGGTAGG 4375 0.0 23.897524 16-17 CGGGGGG 8475 0.0 23.770844 38-39 >>END_MODULE