FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l03n02 tsrexp_lib#19.3420000000cc82.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l03n02 tsrexp_lib#19.3420000000cc82.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8812976
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCCC3900.027.40408144-45
GGGCGCC12750.026.26747142-43
GGGGCGC11850.024.25360942-43
TCGGGGG21800.022.77739138-39
GGGGGCG14050.020.79896540-41
CTCGGGG19300.017.72640436-37
GGGGGGC16800.017.6772140-41
GGGGCCC6200.017.62111944-45
GGGCCCG3550.017.39451644-45
GAGCGGC20700.016.9811129
CGGGTAG11350.016.53680614-15
GTAGGGG15000.016.47429818-19
AGGGTAG21950.016.23034928-29
CGGGGGT9650.015.5105638-39
GCGCCCG5850.015.02149744-45
GGGCGGG12200.014.40655712-13
GGGGGTC12100.014.33344240-41
CGGGGGG22500.014.04375938-39
GGGTAGG14400.013.86054916-17
GGGTAGA27400.013.7821728-29
Produced by FastQC (version 0.11.2)