##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C5B16ACXX l03n02 tsrexp_lib#16.3420000000cbfd.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 22663879 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.617910067380784 33.0 33.0 33.0 33.0 33.0 2 32.56451192666533 33.0 33.0 33.0 33.0 33.0 3 32.552333914243015 33.0 33.0 33.0 33.0 33.0 4 36.440072504799375 37.0 37.0 37.0 37.0 37.0 5 36.441622901357704 37.0 37.0 37.0 37.0 37.0 6 36.46171037182117 37.0 37.0 37.0 37.0 37.0 7 36.46316091786406 37.0 37.0 37.0 37.0 37.0 8 36.48954073572313 37.0 37.0 37.0 37.0 37.0 9 36.44849149609385 37.0 37.0 37.0 37.0 37.0 10-11 36.43923729914019 37.0 37.0 37.0 37.0 37.0 12-13 36.43823608041677 37.0 37.0 37.0 37.0 37.0 14-15 38.82222343756777 40.0 40.0 40.0 37.0 40.0 16-17 38.81040198370279 40.0 40.0 40.0 37.0 40.0 18-19 38.80595267915082 40.0 40.0 40.0 37.0 40.0 20-21 38.78140191712107 40.0 40.0 40.0 37.0 40.0 22-23 38.794228207801496 40.0 40.0 40.0 37.0 40.0 24-25 38.74808067939297 40.0 40.0 40.0 37.0 40.0 26-27 38.73835544656764 40.0 40.0 40.0 37.0 40.0 28-29 38.68773858173175 40.0 40.0 40.0 37.0 40.0 30-31 38.66709524878773 40.0 40.0 40.0 37.0 40.0 32-33 38.634148991000174 40.0 40.0 40.0 37.0 40.0 34-35 38.60215142341697 40.0 40.0 40.0 37.0 40.0 36-37 38.57844027935377 40.0 40.0 40.0 37.0 40.0 38-39 38.5454563404614 40.0 40.0 40.0 37.0 40.0 40-41 38.49745065705655 40.0 40.0 40.0 37.0 40.0 42-43 38.4288027437845 40.0 38.5 40.0 37.0 40.0 44-45 38.38088751268042 40.0 37.0 40.0 37.0 40.0 46-47 38.293199963695535 40.0 37.0 40.0 37.0 40.0 48-49 38.208216828196086 40.0 37.0 40.0 37.0 40.0 50-51 36.8498858690518 38.5 37.0 38.5 35.0 40.0 52-53 37.175238890041726 38.5 37.0 40.0 33.0 40.0 54-55 37.82670177510214 40.0 37.0 40.0 37.0 40.0 56-57 37.78548826527003 40.0 37.0 40.0 37.0 40.0 58-59 37.60595533977215 40.0 37.0 40.0 35.0 40.0 60-61 37.414018624084605 37.0 37.0 40.0 33.0 40.0 62-63 37.19200572417458 37.0 37.0 40.0 33.0 40.0 64-65 36.933410406047436 37.0 37.0 40.0 33.0 40.0 66-67 36.65316078505361 37.0 37.0 40.0 33.0 40.0 68-69 36.3587995682469 37.0 37.0 37.0 33.0 40.0 70-71 36.09779089448898 37.0 37.0 37.0 33.0 40.0 72-73 35.8745288041822 37.0 37.0 37.0 33.0 40.0 74-75 35.66570466600179 37.0 37.0 37.0 33.0 37.0 76-77 35.49243141476356 37.0 37.0 37.0 33.0 37.0 78-79 35.366705849426744 37.0 37.0 37.0 33.0 37.0 80-81 35.267232387712625 37.0 37.0 37.0 33.0 37.0 82-83 35.186299397380296 37.0 37.0 37.0 33.0 37.0 84-85 35.12170275441375 37.0 37.0 37.0 33.0 37.0 86-87 35.06411477488033 37.0 37.0 37.0 33.0 37.0 88-89 34.999309518022045 37.0 33.0 37.0 33.0 37.0 90-91 34.93074437963598 37.0 33.0 37.0 33.0 37.0 92-93 34.89210794409907 37.0 33.0 37.0 33.0 37.0 94-95 34.83846092718726 37.0 33.0 37.0 33.0 37.0 96-97 34.791064495181956 37.0 33.0 37.0 33.0 37.0 98-99 34.73914083727679 37.0 33.0 37.0 33.0 37.0 100-101 32.938382414590194 35.0 30.0 37.0 27.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.18956793762723123 2306 2 0.209217423586729 2306 3 0.2072560561172736 2306 4 0.2611948924354621 2306 5 0.25926119404622483 2306 6 0.28487303111896267 2306 7 0.22211848309529358 2306 8 0.2608192084061187 2306 9 0.25136349301656224 2306 10-11 0.2550927194993875 2306 12-13 0.25259508469117264 2306 14-15 0.46149090737017673 2306 16-17 0.4591632334971649 2306 18-19 0.4673932946092805 2306 20-21 0.4781847493753233 2306 22-23 0.5000018096416383 2306 24-25 0.5176553496233254 2306 26-27 0.5185947953400003 2306 28-29 0.5298135860100928 2306 30-31 0.5067843617924837 2306 32-33 0.5112175886319292 2306 34-35 0.5375802866893054 2306 36-37 0.5792920129122621 2306 38-39 0.5465875011511585 2306 40-41 0.5526810692413946 2306 42-43 0.5857722623011128 2306 44-45 0.5936562763370006 2306 46-47 0.5813195546948151 2306 48-49 0.6120379021455662 2306 50-51 0.2989912297081645 2306 52-53 0.4371117455676483 2306 54-55 0.6077573909227283 2306 56-57 0.6158434847702736 2306 58-59 0.6085378707961908 2306 60-61 0.652932176029033 2306 62-63 0.6351659997364862 2306 64-65 0.6503177351147755 2306 66-67 0.6392152150272068 2306 68-69 0.6428587842201097 2306 70-71 0.6349689214653367 2306 72-73 0.6611767137448723 2306 74-75 0.6407318828732542 2306 76-77 0.6052597797585619 2306 78-79 0.6512609342652809 2306 80-81 0.6974385715224258 2306 82-83 0.7140061845538597 2306 84-85 0.6944345066283617 2306 86-87 0.7189696458607102 2306 88-89 0.7545419673619733 2306 90-91 0.7354759000897886 2306 92-93 0.7779107493156587 2306 94-95 0.7479458574541908 2306 96-97 0.7448298135899449 2306 98-99 0.7812395863133119 2306 100-101 0.6625209127858014 2307 1 0.14624497578222417 2307 2 0.17810723769252945 2307 3 0.18059945179586379 2307 4 0.2036088897913615 2307 5 0.19790909513413624 2307 6 0.21403528725703325 2307 7 0.17049244464089952 2307 8 0.186639348403574 2307 9 0.19203331210427876 2307 10-11 0.17400136338790873 2307 12-13 0.20008045200607683 2307 14-15 0.3415849434552456 2307 16-17 0.3569207008657145 2307 18-19 0.3918710462804711 2307 20-21 0.3911462052415189 2307 22-23 0.4136425855928678 2307 24-25 0.41523002959648636 2307 26-27 0.37844788041851274 2307 28-29 0.3941485558455895 2307 30-31 0.3893291951365967 2307 32-33 0.4314008364234567 2307 34-35 0.41997439968288575 2307 36-37 0.4119070164956966 2307 38-39 0.4226929802109751 2307 40-41 0.4395270085038234 2307 42-43 0.46026406688314836 2307 44-45 0.4265991811732235 2307 46-47 0.41267022421314437 2307 48-49 0.4445192930421129 2307 50-51 0.38175134714899883 2307 52-53 0.42687805296115755 2307 54-55 0.45764880310613876 2307 56-57 0.47625266934479527 2307 58-59 0.4442323411626532 2307 60-61 0.4669161654216154 2307 62-63 0.4728296940535728 2307 64-65 0.4696622480399455 2307 66-67 0.4893344150096297 2307 68-69 0.46903256468820587 2307 70-71 0.45442328497798457 2307 72-73 0.5156298988460222 2307 74-75 0.483052608094269 2307 76-77 0.4706071407822421 2307 78-79 0.48259890621192625 2307 80-81 0.5516393072410324 2307 82-83 0.5497175791940023 2307 84-85 0.49244364964845744 2307 86-87 0.5171677940692092 2307 88-89 0.5612824374851115 2307 90-91 0.5569346809601328 2307 92-93 0.5497166629328447 2307 94-95 0.5548230404278698 2307 96-97 0.5388438674089926 2307 98-99 0.5603112268511055 2307 100-101 0.6243861141072884 2201 1 0.23312546584533322 2201 2 0.22513125931063627 2201 3 0.2432477463934859 2201 4 0.30020202772362126 2201 5 0.2864844006904761 2201 6 0.30117624769842166 2201 7 0.2709654941960693 2201 8 0.2934835029426708 2201 9 0.29612206415090014 2201 10-11 0.25977460821850684 2201 12-13 0.2744931964335109 2201 14-15 0.48719320589818693 2201 16-17 0.47542855814267426 2201 18-19 0.5137018815992747 2201 20-21 0.5230890611065391 2201 22-23 0.5280239493404721 2201 24-25 0.5155694450959842 2201 26-27 0.5098684652809311 2201 28-29 0.5424221976989614 2201 30-31 0.532831971108088 2201 32-33 0.5323499441166746 2201 34-35 0.539835134760196 2201 36-37 0.584731386209306 2201 38-39 0.6034027085475415 2201 40-41 0.5651415206418946 2201 42-43 0.5834175359503249 2201 44-45 0.6008510162271179 2201 46-47 0.6012303262879044 2201 48-49 0.57074541949153 2201 50-51 0.5288859292071422 2201 52-53 0.5907311965177158 2201 54-55 0.643629739777964 2201 56-57 0.6578375940919017 2201 58-59 0.6376594325807545 2201 60-61 0.648068596123359 2201 62-63 0.6369826879592182 2201 64-65 0.6036618400821965 2201 66-67 0.6154556336856487 2201 68-69 0.6499589356051985 2201 70-71 0.6397934570550916 2201 72-73 0.6351030810976326 2201 74-75 0.6477717594015218 2201 76-77 0.6573952789537074 2201 78-79 0.6748965367026756 2201 80-81 0.6723661848516755 2201 82-83 0.6647294561228776 2201 84-85 0.7067606201116803 2201 86-87 0.7321921975792662 2201 88-89 0.7406427220485341 2201 90-91 0.7148415133874053 2201 92-93 0.7342162212091168 2201 94-95 0.7512464377937462 2201 96-97 0.7408729625972086 2201 98-99 0.7673883186055761 2201 100-101 0.7445633422594398 1101 1 0.16319915203536084 1101 2 0.15936325779561855 1101 3 0.17550976135592578 1101 4 0.22098145338512865 1101 5 0.20514361011626647 1101 6 0.23213954842760387 1101 7 0.19977769562265735 1101 8 0.22936102121710178 1101 9 0.22085179952722456 1101 10-11 0.22445039482150975 1101 12-13 0.20890641940527388 1101 14-15 0.41754951925635453 1101 16-17 0.3842660567662364 1101 18-19 0.3930455958256971 1101 20-21 0.40047348648292314 1101 22-23 0.4104974910519985 1101 24-25 0.4390133298698231 1101 26-27 0.4653792022558889 1101 28-29 0.42227397098746877 1101 30-31 0.3870001363747946 1101 32-33 0.43769751641774235 1101 34-35 0.4789792543033258 1101 36-37 0.4915263835624515 1101 38-39 0.47785019360575376 1101 40-41 0.4688645069655095 1101 42-43 0.498259787770877 1101 44-45 0.48888449351302654 1101 46-47 0.47798260127147785 1101 48-49 0.4816562196282064 1101 50-51 0.42525465099438264 1101 52-53 0.5037214633925515 1101 54-55 0.5422132267812145 1101 56-57 0.5247785423550724 1101 58-59 0.5061105452138932 1101 60-61 0.5156050243727393 1101 62-63 0.4825789282714297 1101 64-65 0.5079817868742893 1101 66-67 0.4777346158029516 1101 68-69 0.49687591620657656 1101 70-71 0.5145242888165242 1101 72-73 0.5077484465442197 1101 74-75 0.5482339007370882 1101 76-77 0.5725908742643213 1101 78-79 0.5566447042279989 1101 80-81 0.5564461481622232 1101 82-83 0.546205748094188 1101 84-85 0.536806941191962 1101 86-87 0.5728790345018098 1101 88-89 0.5911908597323077 1101 90-91 0.6258760166164521 1101 92-93 0.6632895569286106 1101 94-95 0.6190602545817683 1101 96-97 0.6030980365250329 1101 98-99 0.6542378604449723 1101 100-101 0.6737825261869759 2304 1 0.1602218279377965 2304 2 0.17637299866084533 2304 3 0.17868452124452716 2304 4 0.22596156194977368 2304 5 0.21703828781586765 2304 6 0.25728014302912783 2304 7 0.23597653080157244 2304 8 0.24764091882426698 2304 9 0.2306444144413362 2304 10-11 0.2322157812915151 2304 12-13 0.22715496910239352 2304 14-15 0.3801824527577651 2304 16-17 0.4216250030948956 2304 18-19 0.40883198122207887 2304 20-21 0.3886411588105787 2304 22-23 0.43531456663659185 2304 24-25 0.428392946502413 2304 26-27 0.4449630799148565 2304 28-29 0.4404961331132782 2304 30-31 0.41458447286510136 2304 32-33 0.43894966808390734 2304 34-35 0.4588241697891675 2304 36-37 0.448240225912393 2304 38-39 0.4810146035567655 2304 40-41 0.49049053462607617 2304 42-43 0.49869280388100634 2304 44-45 0.5391766526960211 2304 46-47 0.5274337445580173 2304 48-49 0.5297221883179759 2304 50-51 0.40119666224958195 2304 52-53 0.47966968699508783 2304 54-55 0.5310639592181587 2304 56-57 0.5660785732140994 2304 58-59 0.5116279562413979 2304 60-61 0.5326024597822183 2304 62-63 0.5390868688675567 2304 64-65 0.5554088377001136 2304 66-67 0.534781504358925 2304 68-69 0.5309793302074155 2304 70-71 0.5160217745448179 2304 72-73 0.49917738659137 2304 74-75 0.5361976745246579 2304 76-77 0.5577491829986698 2304 78-79 0.5698948183396411 2304 80-81 0.5144052859408674 2304 82-83 0.5767147766261544 2304 84-85 0.521634576574634 2304 86-87 0.5758331308592517 2304 88-89 0.5986455453684485 2304 90-91 0.6100157507899908 2304 92-93 0.6181037326228562 2304 94-95 0.6329462459650799 2304 96-97 0.6408681901667563 2304 98-99 0.5995728645539486 2304 100-101 0.582149127176848 2202 1 0.1371528578126231 2202 2 0.15039394359541092 2202 3 0.1564904599774053 2202 4 0.20791686495358164 2202 5 0.1847712860876527 2202 6 0.2403119299314156 2202 7 0.20276150935350756 2202 8 0.21607758223538553 2202 9 0.22299035652842747 2202 10-11 0.21452802888549627 2202 12-13 0.21890421283725203 2202 14-15 0.3833366142563577 2202 16-17 0.3725741528403219 2202 18-19 0.40534829170647413 2202 20-21 0.3500021221663516 2202 22-23 0.3479582166551083 2202 24-25 0.36856958923226557 2202 26-27 0.40476937917971156 2202 28-29 0.3974468935936031 2202 30-31 0.4161009492659531 2202 32-33 0.4541596510528123 2202 34-35 0.43705839718798245 2202 36-37 0.41680380195908384 2202 38-39 0.4603177903186051 2202 40-41 0.4591677679288253 2202 42-43 0.4816071398699009 2202 44-45 0.48722626440667227 2202 46-47 0.48107529685443495 2202 48-49 0.4669663787757088 2202 50-51 0.48449092898405155 2202 52-53 0.4798043932198226 2202 54-55 0.4483420594119636 2202 56-57 0.4703082231124469 2202 58-59 0.48247444405909334 2202 60-61 0.46547032122673215 2202 62-63 0.4529131624105318 2202 64-65 0.49793592468248704 2202 66-67 0.4583215068162829 2202 68-69 0.4521601979773777 2202 70-71 0.47367762614914 2202 72-73 0.4920974354874019 2202 74-75 0.494058383624683 2202 76-77 0.45808889918287576 2202 78-79 0.49395930513313857 2202 80-81 0.5292806288722502 2202 82-83 0.5444303581543508 2202 84-85 0.525797303846538 2202 86-87 0.5004451957657707 2202 88-89 0.5524553817927682 2202 90-91 0.52231723623553 2202 92-93 0.534247861108561 2202 94-95 0.5733941127727391 2202 96-97 0.5804845375115093 2202 98-99 0.5468714487036337 2202 100-101 0.6558334141710631 1102 1 0.1036999143434798 1102 2 0.1313393374308447 1102 3 0.14170372117190055 1102 4 0.16883359623704308 1102 5 0.17847752900024005 1102 6 0.15256012156731202 1102 7 0.15417368814320298 1102 8 0.1664915155250739 1102 9 0.17879988273126912 1102 10-11 0.16504912013729722 1102 12-13 0.1601615827561318 1102 14-15 0.2767526683622634 1102 16-17 0.27609188869040935 1102 18-19 0.29839755116861966 1102 20-21 0.28991642285961206 1102 22-23 0.28792572179767717 1102 24-25 0.30445215959611005 1102 26-27 0.29611566454568106 1102 28-29 0.31729552477672485 1102 30-31 0.3252099318017372 1102 32-33 0.3589457527516302 1102 34-35 0.3453774318658347 1102 36-37 0.34573240195216925 1102 38-39 0.38074205987900456 1102 40-41 0.39438781982684645 1102 42-43 0.3869543158965172 1102 44-45 0.34951527033739893 1102 46-47 0.3732640547323882 1102 48-49 0.3747037245763636 1102 50-51 0.37083866858026227 1102 52-53 0.39850448132740723 1102 54-55 0.4088563921780235 1102 56-57 0.3932750751199663 1102 58-59 0.4047271582664891 1102 60-61 0.4155564369483429 1102 62-63 0.36555016471321977 1102 64-65 0.3831306268222292 1102 66-67 0.3977114372927204 1102 68-69 0.3711801732453992 1102 70-71 0.3618038631610503 1102 72-73 0.4135770291856957 1102 74-75 0.3988624435165775 1102 76-77 0.4077470177464164 1102 78-79 0.41668145984568383 1102 80-81 0.4479859298173636 1102 82-83 0.482313622328185 1102 84-85 0.4722646004192228 1102 86-87 0.5062079151658594 1102 88-89 0.4843333024859504 1102 90-91 0.4178981579267429 1102 92-93 0.44000570112519455 1102 94-95 0.48762660258808666 1102 96-97 0.48710700298875054 1102 98-99 0.472234002278789 1102 100-101 0.5332063115881027 2305 1 0.1303441264479801 2305 2 0.1348277610173838 2305 3 0.14514317927568499 2305 4 0.1637130857276432 2305 5 0.15628177043211622 2305 6 0.20639956795868386 2305 7 0.16166377128612908 2305 8 0.20742996683160442 2305 9 0.20322502225547368 2305 10-11 0.1880342342760457 2305 12-13 0.1972979907739827 2305 14-15 0.32680629466560873 2305 16-17 0.3243955739989488 2305 18-19 0.3426833858204006 2305 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62-63 -1.0254606397271218 1213 64-65 -0.9243919579009798 1213 66-67 -1.0944417941684534 1213 68-69 -1.0911146538492176 1213 70-71 -1.06855330557525 1213 72-73 -1.0529811943231735 1213 74-75 -1.0792345255094844 1213 76-77 -1.0539015085824062 1213 78-79 -1.088804794854397 1213 80-81 -1.1187559108403633 1213 82-83 -1.149696335681 1213 84-85 -1.1098046198907738 1213 86-87 -1.1862096417176815 1213 88-89 -1.2457504228108291 1213 90-91 -1.3868163747700706 1213 92-93 -1.2627295688907836 1213 94-95 -1.1883374359809764 1213 96-97 -1.3077649780687324 1213 98-99 -1.294962432313575 1213 100-101 -1.2429635529170255 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 20879.0 3 1688.0 4 1178.0 5 925.0 6 867.0 7 1500.0 8 2862.0 9 5736.0 10 15590.0 11 27729.0 12 25847.0 13 22701.0 14 21934.0 15 21351.0 16 22153.0 17 24540.0 18 27329.0 19 30383.0 20 33747.0 21 37621.0 22 42948.0 23 48825.0 24 55217.0 25 62707.0 26 70971.0 27 84436.0 28 101158.0 29 125462.0 30 157835.0 31 206081.0 32 276795.0 33 382229.0 34 545723.0 35 827419.0 36 1712464.0 37 8255162.0 38 9358323.0 39 3564.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 27.73692448774481 15.22945558145778 18.7744490151824 38.25917091561501 2 24.844017270365413 26.479408158976998 29.749359324298986 18.927215246358607 3 22.635357275824276 28.065301413723226 29.835798796765307 19.463542513687187 4 25.12375666181922 31.201091668143523 21.939524274783555 21.735627395253708 5 26.920005818105725 33.67126182730583 21.940877300356526 17.467855054231922 6 19.484155181961334 37.86743086812246 22.50496714011786 20.14344680979835 7 18.403921119753957 17.37482995700645 42.194270616336574 22.026978306903015 8 22.073178397828634 22.504060531125024 28.2280454889042 27.194715582142138 9 24.251560509409224 23.402107769525756 27.34448174246734 25.00184997859768 10-11 24.103198789110607 31.7712485666154 22.16440141609599 21.961151228178004 12-13 23.01705678650403 25.984084572529188 27.67396505610679 23.324893584859993 14-15 22.974522014488898 27.310042265451905 27.653469913994723 22.061965806064475 16-17 23.832026128526504 27.53895194283732 26.79038766156745 21.83863426706873 18-19 23.696512981947055 27.75811896862247 26.838676195605814 21.706691853824662 20-21 23.767915589787787 27.9137239442756 26.711258307888137 21.607102158048484 22-23 24.02202186839065 27.704389496545502 26.69104088820702 21.58254774685683 24-25 23.360586871517434 28.218770024406066 26.758275008991063 21.662368095085437 26-27 23.667531419944737 28.383500651497744 26.515590269503047 21.43337765905447 28-29 23.796504239865094 27.789854474460864 26.793905373036697 21.61973591263735 30-31 23.5796722006766 27.76637866398627 26.875819601557556 21.778129533779573 32-33 23.608244660472018 27.99793922584948 26.745573182298838 21.648242931379663 34-35 23.768696326943896 27.851712943871217 26.688483627481812 21.691107101703075 36-37 23.312540264060882 27.787753177421358 27.04810620369364 21.851600354824118 38-39 23.60516140303677 27.788810618084746 26.820443872849275 21.785584106029205 40-41 24.090001244642202 27.545330876815072 26.710704577100984 21.653963301441735 42-43 23.813042953834575 27.524086213335035 26.967992601268175 21.694878231562214 44-45 24.064499494844693 27.65682055098103 26.68536005291533 21.593319901258944 46-47 24.019898160617185 27.53134294735667 26.700627854089316 21.74813103793683 48-49 23.614495834199996 27.53956309155507 26.88133120711668 21.964609867128253 50-51 24.112699811967143 27.697192619385753 26.675300191213715 21.51480737743339 52-53 24.09035751699063 27.553524834456283 26.818332375220272 21.537785273332812 54-55 23.852824827013006 27.64262128878801 26.730404436721972 21.774149447477008 56-57 24.047905963676317 27.64718269228115 26.829687254136037 21.4752240899065 58-59 24.26194581745921 27.840682824496355 26.527059796397474 21.370311561646957 60-61 24.090751299120765 27.970151315407744 26.517880150842966 21.42121723462852 62-63 24.34060767220376 28.028817570386217 26.245671218562876 21.384903538847148 64-65 24.55835044995772 27.928265805329062 26.263676016127153 21.249707728586063 66-67 24.32958670374735 28.016925537520383 26.390736525599024 21.262751233133244 68-69 24.71170847009163 28.010885902658433 26.103436616194344 21.173969011055597 70-71 24.918450709111642 27.93146990961291 26.018493022678157 21.131586358597286 72-73 24.791903601405078 27.983435370092568 26.150402191208826 21.074258837293527 74-75 25.163023439790273 28.066225586911557 25.857614700950094 20.913136272348083 76-77 25.41139890799235 27.847890072729424 25.85328635724984 20.887424662028383 78-79 25.332811071433863 28.015451099592003 25.87309632301305 20.778641505961083 80-81 25.809253522305546 28.044887579051682 25.474994843957283 20.670864054685484 82-83 26.024564759819384 27.91700642298183 25.50550096570342 20.55292785149536 84-85 25.954795628764483 27.99980334383072 25.548644540064753 20.496756487340047 86-87 26.317956692445293 28.082509276676852 25.211076825159296 20.38845720571856 88-89 26.67290690832789 27.947397042363125 25.232509782977026 20.147186266331957 90-91 26.638953523604513 28.03416863431794 25.21889980035659 20.10797804172096 92-93 27.02899897871897 28.10714521019772 24.939475532137244 19.924380278946064 94-95 27.348733586649427 27.97616999298468 24.905355441278584 19.76974097908731 96-97 27.303247751704834 28.10820078828869 24.870010375846473 19.71854108416 98-99 27.71884707352314 28.17481546865432 24.66310374850663 19.443233709315912 100-101 27.968755831340143 28.126780870576436 24.52780526520466 19.376658032878765 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 17822.0 1 9753.5 2 1318.0 3 846.5 4 693.0 5 604.0 6 561.0 7 565.0 8 568.5 9 560.0 10 561.0 11 595.0 12 688.5 13 776.0 14 866.0 15 982.5 16 1122.5 17 1256.0 18 1425.0 19 1714.5 20 2162.5 21 2691.5 22 3436.5 23 4724.5 24 6696.5 25 9679.0 26 14369.0 27 21164.0 28 30546.0 29 44346.5 30 60163.0 31 78650.0 32 102930.0 33 131228.5 34 166669.0 35 216730.5 36 287868.0 37 374246.5 38 479198.0 39 609154.0 40 761769.0 41 926155.0 42 1097648.0 43 1266275.5 44 1408315.0 45 1513753.0 46 1576328.0 47 1589597.5 48 1549883.0 49 1459832.5 50 1324896.5 51 1159233.0 52 977674.5 53 808381.0 54 644761.0 55 492500.0 56 373721.5 57 281696.0 58 211086.0 59 154616.5 60 110000.0 61 76099.5 62 55136.0 63 40036.0 64 28156.5 65 22035.0 66 17281.5 67 12492.5 68 9949.0 69 8439.5 70 7543.5 71 5761.0 72 3205.5 73 2120.5 74 2083.5 75 1677.5 76 1244.5 77 601.0 78 206.0 79 122.0 80 90.0 81 70.0 82 62.0 83 56.0 84 42.5 85 38.0 86 37.0 87 27.0 88 24.0 89 23.0 90 20.5 91 15.0 92 9.0 93 8.5 94 5.0 95 3.0 96 2.5 97 2.0 98 1.5 99 2.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.13255894986026 2 0.132205965271876 3 0.12968653777228514 4 0.1383699586465318 5 0.13714774950925215 6 0.12184145529545053 7 0.12218561526912493 8 0.11940586163560085 9 0.12567133807941702 10-11 0.08982354697534345 12-13 0.09712150334018285 14-15 0.1497117947020455 16-17 0.15160467455725474 18-19 0.15159584994254516 20-21 0.1826827614107894 22-23 0.15527130196909364 24-25 0.15205914221479913 26-27 0.14030034311425685 28-29 0.15093841614667994 30-31 0.10787429636383075 32-33 0.12233342756551074 34-35 0.13556152501520152 36-37 0.13791769714266477 38-39 0.1398679369934864 40-41 0.17165199302378908 42-43 0.15264597909298755 44-45 0.13563653424023311 46-47 0.12761495946920648 48-49 0.15007139775146172 50-51 0.12563162731322383 52-53 0.1246101781605876 54-55 0.11111513611593143 56-57 0.10453417969624706 58-59 0.11535536348389434 60-61 0.11544581578466775 62-63 0.10012407849512434 64-65 0.1170585141228472 66-67 0.10853393631337337 68-69 0.11542816655524855 70-71 0.09797307865965928 72-73 0.10408853665341224 74-75 0.13582846961016692 76-77 0.16658887033415593 78-79 0.13360246054967026 80-81 0.12273935984215235 82-83 0.1134735143970721 84-85 0.11199539143321405 86-87 0.11616502188350017 88-89 0.12356004901014517 90-91 0.14021209696716083 92-93 0.11530903425666895 94-95 0.11598191112827595 96-97 0.1135110190095879 98-99 0.10511439811340328 100-101 0.09801499557952989 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2.2663879E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.37724325017179 #Duplication Level Percentage of deduplicated Percentage of total 1 63.620249068535315 24.41569774139695 2 15.883624576739642 12.191394481518882 3 7.2475839007458704 8.344268710048594 4 3.6891574579548476 5.663187726084743 5 2.2333075491469114 4.285409353302801 6 1.5269039777449371 3.5159019224143404 7 1.077733830908649 2.8952317371404512 8 0.7772164171182437 2.3861938798219056 9 0.6405975910936093 2.212593262098617 >10 3.0069241989744073 20.983787378611524 >50 0.19993274419613738 5.253480491985905 >100 0.09220194787814125 6.304739569540977 >500 0.003615795130987007 0.9709131810937582 >1k 9.263501964392475E-4 0.47783150160450977 >5k 1.3063492877063529E-5 0.02983980702535404 >10k+ 1.1530143138602113E-5 0.06952925631052302 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 2.6915074864280737E-4 0.0 4.41230735480012E-6 2 3.220984369004088E-4 0.0 8.82461470960024E-6 3 4.1916919870601144E-4 8.82461470960024E-6 8.82461470960024E-6 4 6.530214885104178E-4 1.3236922064400361E-5 8.82461470960024E-6 5 7.368553282516201E-4 1.3236922064400361E-5 8.82461470960024E-6 6 8.956983930244244E-4 1.3236922064400361E-5 1.3236922064400361E-5 7 9.486460812820259E-4 1.3236922064400361E-5 1.3236922064400361E-5 8 0.0010457168430876285 1.3236922064400361E-5 1.3236922064400361E-5 9 0.0011957352931508327 1.3236922064400361E-5 1.3236922064400361E-5 10-11 0.001570781418308843 2.20615367740006E-5 1.3236922064400361E-5 12-13 0.0020075998464340545 2.6473844128800722E-5 1.3236922064400361E-5 14-15 0.0024532428892688673 2.6473844128800722E-5 1.3236922064400361E-5 16-17 0.00292094746887768 3.309230516100091E-5 1.3236922064400361E-5 18-19 0.0035033720397112957 4.853538090280132E-5 1.3236922064400361E-5 20-21 0.004335091976091119 6.177230296720169E-5 1.3236922064400361E-5 22-23 0.005945584160593162 7.059691767680193E-5 1.3236922064400361E-5 24-25 0.00803260553941362 7.942153238640217E-5 1.3236922064400361E-5 26-27 0.012365491361827338 9.045230077340248E-5 1.3236922064400361E-5 28-29 0.02237481059619141 1.0810153019260296E-4 1.3236922064400361E-5 30-31 0.040321870761840906 1.1471999122480314E-4 1.3236922064400361E-5 32-33 0.07069178228493013 1.1913229857960326E-4 1.3236922064400361E-5 34-35 0.11038269309503462 1.2354460593440338E-4 1.3236922064400361E-5 36-37 0.1691766885977462 1.323692206440036E-4 1.3236922064400361E-5 38-39 0.24753706106531898 1.3678152799880374E-4 1.3236922064400361E-5 40-41 0.34651173349451786 1.433999890310039E-4 1.3236922064400361E-5 42-43 0.4742017021887559 1.500184500632041E-4 1.3236922064400361E-5 44-45 0.6221044508753334 1.544307574180042E-4 1.3236922064400361E-5 46-47 0.7950933730276268 1.566369110954043E-4 1.3236922064400361E-5 48-49 0.9964931422374783 1.6325537212760446E-4 1.3236922064400361E-5 50-51 1.232867065695153 1.6766767948240457E-4 1.3236922064400361E-5 52-53 1.5134964319214728 1.7649229419200484E-4 1.544307574180042E-5 54-55 1.836141994933877 1.9855383096600542E-4 2.20615367740006E-5 56-57 2.1994734440648926 2.0296613832080556E-4 2.6473844128800722E-5 58-59 2.6131802062656617 2.1179075303040578E-4 2.6473844128800722E-5 60-61 3.0797199367328076 2.1399690670780583E-4 2.6473844128800722E-5 62-63 3.6058589087949153 2.1840921406260597E-4 2.6473844128800722E-5 64-65 4.186882572043382 2.2061536774000602E-4 2.6473844128800722E-5 66-67 4.81822418836599 2.2061536774000602E-4 2.6473844128800722E-5 68-69 5.511172646129994 2.3385228980440638E-4 2.8679997806200784E-5 70-71 6.268432689743888 2.3826459715920652E-4 3.0886151483600846E-5 72-73 7.095625598777685 2.448830581914067E-4 3.0886151483600846E-5 74-75 7.987584561318917 2.515015192236069E-4 3.529845883840096E-5 76-77 8.931469762965111 2.713569023202074E-4 3.529845883840096E-5 78-79 9.940679174999126 2.779753633524076E-4 3.529845883840096E-5 80-81 11.024200667502681 2.779753633524076E-4 3.529845883840096E-5 82-83 12.180136948313216 2.8459382438460776E-4 3.529845883840096E-5 84-85 13.402562288653236 2.8900613173940787E-4 3.9710766193201086E-5 86-87 14.686720221194262 3.000369001264082E-4 3.9710766193201086E-5 88-89 16.032928432065845 3.0665536115860836E-4 3.9710766193201086E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGCGCCC 1170 0.0 32.479332 44-45 GGGCGCC 4115 0.0 28.05321 42-43 GGGGGCG 4350 0.0 23.15573 40-41 TCGGGGG 6785 0.0 22.828619 38-39 GGGGCGC 4310 0.0 22.705765 42-43 CGGGTAG 2500 0.0 19.95713 14-15 GGGGGGC 4960 0.0 19.828989 40-41 GGGCCCG 1000 0.0 18.762903 44-45 GTAGGGG 4130 0.0 18.295101 18-19 AGGGTAG 6125 0.0 18.03074 28-29 GGGTAGA 6685 0.0 17.87036 28-29 GGGGCCC 1720 0.0 17.536713 44-45 GCGCCCG 1900 0.0 17.375374 44-45 CTCGGGG 5510 0.0 17.246132 36-37 GGGCGGG 3620 0.0 16.993914 12-13 GAGCGGC 5560 0.0 16.830492 9 AAGGGGC 4790 0.0 15.569315 9 CGGGGGG 6390 0.0 15.540226 38-39 CGGGGGT 2675 0.0 14.9199505 38-39 TAGGGGA 5445 0.0 14.356737 18-19 >>END_MODULE