FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l03n02 tsrexp_lib#16.3420000000cbfd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l03n02 tsrexp_lib#16.3420000000cbfd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22663879
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCCC11700.032.47933244-45
GGGCGCC41150.028.0532142-43
GGGGGCG43500.023.1557340-41
TCGGGGG67850.022.82861938-39
GGGGCGC43100.022.70576542-43
CGGGTAG25000.019.9571314-15
GGGGGGC49600.019.82898940-41
GGGCCCG10000.018.76290344-45
GTAGGGG41300.018.29510118-19
AGGGTAG61250.018.0307428-29
GGGTAGA66850.017.8703628-29
GGGGCCC17200.017.53671344-45
GCGCCCG19000.017.37537444-45
CTCGGGG55100.017.24613236-37
GGGCGGG36200.016.99391412-13
GAGCGGC55600.016.8304929
AAGGGGC47900.015.5693159
CGGGGGG63900.015.54022638-39
CGGGGGT26750.014.919950538-39
TAGGGGA54450.014.35673718-19
Produced by FastQC (version 0.11.2)