##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C5B16ACXX l03n02 tsrexp_lib#11.3420000000cb76.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 11055014 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.606367572216556 33.0 33.0 33.0 33.0 33.0 2 32.56585536662369 33.0 33.0 33.0 33.0 33.0 3 32.55618880265552 33.0 33.0 33.0 33.0 33.0 4 36.43989397028353 37.0 37.0 37.0 37.0 37.0 5 36.43953639497879 37.0 37.0 37.0 37.0 37.0 6 36.43718162636429 37.0 37.0 37.0 37.0 37.0 7 36.46896774621904 37.0 37.0 37.0 37.0 37.0 8 36.471617765477276 37.0 37.0 37.0 37.0 37.0 9 36.45447785050295 37.0 37.0 37.0 37.0 37.0 10-11 36.442173931213475 37.0 37.0 37.0 37.0 37.0 12-13 36.4417236377991 37.0 37.0 37.0 37.0 37.0 14-15 38.8226289898864 40.0 40.0 40.0 37.0 40.0 16-17 38.80999454184318 40.0 40.0 40.0 37.0 40.0 18-19 38.80208840079262 40.0 40.0 40.0 37.0 40.0 20-21 38.77688133185539 40.0 40.0 40.0 37.0 40.0 22-23 38.75992689832867 40.0 40.0 40.0 37.0 40.0 24-25 38.73373783153961 40.0 40.0 40.0 37.0 40.0 26-27 38.717318268434575 40.0 40.0 40.0 37.0 40.0 28-29 38.680816550752446 40.0 40.0 40.0 37.0 40.0 30-31 38.66520725346888 40.0 40.0 40.0 37.0 40.0 32-33 38.631305713407514 40.0 40.0 40.0 37.0 40.0 34-35 38.595084094873144 40.0 40.0 40.0 37.0 40.0 36-37 38.56648196013139 40.0 40.0 40.0 37.0 40.0 38-39 38.52690516719382 40.0 40.0 40.0 37.0 40.0 40-41 38.4701014399439 40.0 40.0 40.0 37.0 40.0 42-43 38.411596765051584 40.0 37.0 40.0 37.0 40.0 44-45 38.35075663404859 40.0 37.0 40.0 37.0 40.0 46-47 38.26898030160794 40.0 37.0 40.0 37.0 40.0 48-49 38.164515576371045 40.0 37.0 40.0 37.0 40.0 50-51 36.80802529965136 38.5 37.0 38.5 35.0 40.0 52-53 37.123416306845016 38.5 37.0 40.0 33.0 40.0 54-55 37.759045397862 40.0 37.0 40.0 37.0 40.0 56-57 37.69896121343673 40.0 37.0 40.0 35.0 40.0 58-59 37.51998523023128 40.0 37.0 40.0 33.0 40.0 60-61 37.30571625689483 37.0 37.0 40.0 33.0 40.0 62-63 37.08239577986966 37.0 37.0 40.0 33.0 40.0 64-65 36.83164905987455 37.0 37.0 40.0 33.0 40.0 66-67 36.57221053722772 37.0 37.0 40.0 33.0 40.0 68-69 36.305210694441456 37.0 37.0 37.0 33.0 40.0 70-71 36.06399096373826 37.0 37.0 37.0 33.0 40.0 72-73 35.8561896891311 37.0 37.0 37.0 33.0 38.5 74-75 35.65620310385857 37.0 37.0 37.0 33.0 37.0 76-77 35.48690381577084 37.0 37.0 37.0 33.0 37.0 78-79 35.361284978924495 37.0 37.0 37.0 33.0 37.0 80-81 35.26888627187627 37.0 37.0 37.0 33.0 37.0 82-83 35.186435177739256 37.0 37.0 37.0 33.0 37.0 84-85 35.121451904086236 37.0 37.0 37.0 33.0 37.0 86-87 35.064943924991866 37.0 37.0 37.0 33.0 37.0 88-89 35.00223491349717 37.0 33.0 37.0 33.0 37.0 90-91 34.93129918243432 37.0 33.0 37.0 33.0 37.0 92-93 34.89126368360999 37.0 33.0 37.0 33.0 37.0 94-95 34.83495814659303 37.0 33.0 37.0 33.0 37.0 96-97 34.78533279107562 37.0 33.0 37.0 33.0 37.0 98-99 34.728229245119 37.0 33.0 37.0 33.0 37.0 100-101 32.91407631867314 35.0 30.0 37.0 27.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.19495193399522037 2306 2 0.21594401797862162 2306 3 0.22079784629068655 2306 4 0.29439430573951597 2306 5 0.27157305754460026 2306 6 0.2740095457729197 2306 7 0.23060370939734298 2306 8 0.24807903513818275 2306 9 0.2789067128532352 2306 10-11 0.26135639881784556 2306 12-13 0.2585042333994281 2306 14-15 0.43395701960108113 2306 16-17 0.4766319056744237 2306 18-19 0.4613211461698228 2306 20-21 0.48420722948547734 2306 22-23 0.5041077817943531 2306 24-25 0.5320644245741661 2306 26-27 0.47399190114236944 2306 28-29 0.5182511224893886 2306 30-31 0.5118877005202407 2306 32-33 0.5138746205491245 2306 34-35 0.5575071032010968 2306 36-37 0.5375798794464259 2306 38-39 0.5657082515062726 2306 40-41 0.5423609843051977 2306 42-43 0.5705327960440911 2306 44-45 0.5807051271207371 2306 46-47 0.564040161334681 2306 48-49 0.5904606266938259 2306 50-51 0.23705771941259002 2306 52-53 0.40292808963388893 2306 54-55 0.5921348711927976 2306 56-57 0.6023586741066538 2306 58-59 0.5988760280949705 2306 60-61 0.6374505847265013 2306 62-63 0.6681658034809104 2306 64-65 0.6223915468086716 2306 66-67 0.6378705587660107 2306 68-69 0.6224500474707639 2306 70-71 0.5965877196560427 2306 72-73 0.6001974467367859 2306 74-75 0.6039582340478873 2306 76-77 0.6245740737174472 2306 78-79 0.6325966541247041 2306 80-81 0.667745923852145 2306 82-83 0.696890813139909 2306 84-85 0.6683177426080604 2306 86-87 0.6985653304418022 2306 88-89 0.7580034008902103 2306 90-91 0.7040560737458534 2306 92-93 0.7548654741348528 2306 94-95 0.7797537311157186 2306 96-97 0.7369030478968241 2306 98-99 0.8193950465710813 2306 100-101 0.7170842103852522 2307 1 0.12719386588675974 2307 2 0.16943804027374654 2307 3 0.1506365659129827 2307 4 0.21161213428678138 2307 5 0.18510603779005663 2307 6 0.17997245267021356 2307 7 0.15635529206407028 2307 8 0.16431617026917422 2307 9 0.18567988471744457 2307 10-11 0.16958751157317664 2307 12-13 0.1954174072518029 2307 14-15 0.29195175474113455 2307 16-17 0.32610687934383265 2307 18-19 0.38949490416494825 2307 20-21 0.373304263220092 2307 22-23 0.3982031985727019 2307 24-25 0.41309917592152345 2307 26-27 0.37707258663116505 2307 28-29 0.3791669237384099 2307 30-31 0.39668069865916067 2307 32-33 0.3885935658657971 2307 34-35 0.43948431984681946 2307 36-37 0.4507393365014494 2307 38-39 0.43710033131470283 2307 40-41 0.42428657160007077 2307 42-43 0.42354005787650806 2307 44-45 0.3924678485399369 2307 46-47 0.3894946015469358 2307 48-49 0.4140109215838663 2307 50-51 0.3574423536721767 2307 52-53 0.4301570795835943 2307 54-55 0.4339896647446011 2307 56-57 0.4470423735516036 2307 58-59 0.3920701396058135 2307 60-61 0.4199694754315857 2307 62-63 0.4345451049306348 2307 64-65 0.4183557875375925 2307 66-67 0.4034714939581505 2307 68-69 0.41791445367031343 2307 70-71 0.48614185497451956 2307 72-73 0.4926819724023517 2307 74-75 0.49381553922982135 2307 76-77 0.460185578896116 2307 78-79 0.47460863847609147 2307 80-81 0.4935943225621031 2307 82-83 0.5595899095318941 2307 84-85 0.5104498095563414 2307 86-87 0.5810098789083327 2307 88-89 0.5527680236486532 2307 90-91 0.558235949749843 2307 92-93 0.5516303535929694 2307 94-95 0.552520009838716 2307 96-97 0.5560206805218328 2307 98-99 0.574692233067168 2307 100-101 0.6455525690047565 2201 1 0.22617899981111123 2201 2 0.22871043219533504 2201 3 0.22051518185034524 2201 4 0.2718694086079054 2201 5 0.3037514820183347 2201 6 0.30179414831331997 2201 7 0.2707380843352212 2201 8 0.3013042324268369 2201 9 0.29337070394791454 2201 10-11 0.27119021916093544 2201 12-13 0.2986455656196014 2201 14-15 0.5312240003393498 2201 16-17 0.5271038081929618 2201 18-19 0.5398595205511185 2201 20-21 0.5447339951443269 2201 22-23 0.5556908616838356 2201 24-25 0.5625460672879043 2201 26-27 0.5593015002041355 2201 28-29 0.5813304156295089 2201 30-31 0.587387133998682 2201 32-33 0.5887256353184327 2201 34-35 0.5931772882648119 2201 36-37 0.6227656388834788 2201 38-39 0.6290234959056562 2201 40-41 0.6383235740044455 2201 42-43 0.6835343664020996 2201 44-45 0.6770442051814456 2201 46-47 0.6731836204954362 2201 48-49 0.6254937234795648 2201 50-51 0.6011535708845557 2201 52-53 0.6495506272529141 2201 54-55 0.7287872257359069 2201 56-57 0.6822447734566452 2201 58-59 0.6604591079844511 2201 60-61 0.6295826935781506 2201 62-63 0.6406759909299709 2201 64-65 0.6265247688254831 2201 66-67 0.6571410380233687 2201 68-69 0.6923856429147364 2201 70-71 0.7281332302211752 2201 72-73 0.6889499066330274 2201 74-75 0.6776503643677216 2201 76-77 0.713063349563754 2201 78-79 0.7118391054734232 2201 80-81 0.7307987793195778 2201 82-83 0.718081502308415 2201 84-85 0.7839084158742082 2201 86-87 0.7714730768786708 2201 88-89 0.7754099248331983 2201 90-91 0.8035569384366497 2201 92-93 0.7969323833125088 2201 94-95 0.8163752748372701 2201 96-97 0.85259589382283 2201 98-99 0.8388209713926784 2201 100-101 0.8639168579293326 1101 1 0.2041200210173244 1101 2 0.22965292319190667 1101 3 0.220810272324222 1101 4 0.2813586656160538 1101 5 0.2298455066360745 1101 6 0.2698128689062713 1101 7 0.26531061780683984 1101 8 0.28226989768432986 1101 9 0.2869594470789849 1101 10-11 0.2627675510168217 1101 12-13 0.27429150900818655 1101 14-15 0.4561618431613752 1101 16-17 0.47101650137980045 1101 18-19 0.4624879640088153 1101 20-21 0.46284262507522556 1101 22-23 0.42527679246280314 1101 24-25 0.49561335291183184 1101 26-27 0.47176787371206785 1101 28-29 0.48526894088988826 1101 30-31 0.4524123515779479 1101 32-33 0.5139902092407667 1101 34-35 0.5782638287086925 1101 36-37 0.5495262149293936 1101 38-39 0.5517648464408325 1101 40-41 0.5576312837593136 1101 42-43 0.5657089367307151 1101 44-45 0.5651220504481245 1101 46-47 0.5586386243605901 1101 48-49 0.586103308457794 1101 50-51 0.48131503133593156 1101 52-53 0.5697822099936474 1101 54-55 0.6142901235176979 1101 56-57 0.5868380598486169 1101 58-59 0.6045989142572239 1101 60-61 0.6249181325119153 1101 62-63 0.563780842303963 1101 64-65 0.5648965222057853 1101 66-67 0.5434533696411137 1101 68-69 0.5294031441778628 1101 70-71 0.5741633141287821 1101 72-73 0.5919411448439789 1101 74-75 0.601405880244819 1101 76-77 0.645755443970053 1101 78-79 0.6156093692130327 1101 80-81 0.6174334456558483 1101 82-83 0.6317243076129841 1101 84-85 0.6344377302484787 1101 86-87 0.6295490470219178 1101 88-89 0.6487337459950524 1101 90-91 0.701582063758508 1101 92-93 0.7308918013781067 1101 94-95 0.7300125846646139 1101 96-97 0.6637953766484728 1101 98-99 0.695074508065332 1101 100-101 0.761945613596744 2304 1 0.13567367672490604 2304 2 0.1633052419365555 2304 3 0.1873608812485017 2304 4 0.196203568206748 2304 5 0.22725363886330285 2304 6 0.24260239273218787 2304 7 0.21028085676592667 2304 8 0.221525198261034 2304 9 0.22104342699221746 2304 10-11 0.20052228620266987 2304 12-13 0.18775571403491398 2304 14-15 0.34556034726226414 2304 16-17 0.38269144791661347 2304 18-19 0.38913649981047627 2304 20-21 0.37206617180839885 2304 22-23 0.3987295107269162 2304 24-25 0.4112070612550909 2304 26-27 0.41196990750948714 2304 28-29 0.43019672095228856 2304 30-31 0.39955843989262263 2304 32-33 0.4220387858488479 2304 34-35 0.45358892523868377 2304 36-37 0.4445196269080114 2304 38-39 0.45361992949169405 2304 40-41 0.47564377929531076 2304 42-43 0.4765444060659192 2304 44-45 0.4695160196922643 2304 46-47 0.463963101858873 2304 48-49 0.49911239246981154 2304 50-51 0.3601113288927422 2304 52-53 0.44325635891281223 2304 54-55 0.48129253140548656 2304 56-57 0.5248376079469068 2304 58-59 0.4537386956405669 2304 60-61 0.5153384499030764 2304 62-63 0.467627358058742 2304 64-65 0.510051657287832 2304 66-67 0.4892217057155719 2304 68-69 0.5083997237494273 2304 70-71 0.48940371205230093 2304 72-73 0.45954463851165883 2304 74-75 0.5258924371108478 2304 76-77 0.4854876986299388 2304 78-79 0.536010419563425 2304 80-81 0.49278820947299096 2304 82-83 0.5474769863806515 2304 84-85 0.5094361270162935 2304 86-87 0.5258296168495846 2304 88-89 0.539636009108186 2304 90-91 0.5174628384231781 2304 92-93 0.5663024698373107 2304 94-95 0.6072201197959473 2304 96-97 0.6125998791580329 2304 98-99 0.5665027101052829 2304 100-101 0.6092944069686723 2202 1 0.17483123877159557 2202 2 0.1697380825009276 2202 3 0.18274567660763097 2202 4 0.2264249520391104 2202 5 0.21241206279586322 2202 6 0.27359411018158397 2202 7 0.23071710788296684 2202 8 0.25692822392433357 2202 9 0.2541920631564665 2202 10-11 0.2358826491318382 2202 12-13 0.2608603819976949 2202 14-15 0.42159596663793764 2202 16-17 0.3949201627109886 2202 18-19 0.4402703760250404 2202 20-21 0.3889366321497789 2202 22-23 0.39480450065462946 2202 24-25 0.4318086998246713 2202 26-27 0.4474468069028106 2202 28-29 0.45999237138789795 2202 30-31 0.47091023667497467 2202 32-33 0.4937322544123717 2202 34-35 0.49895151840542695 2202 36-37 0.46897634750624917 2202 38-39 0.5061759405137138 2202 40-41 0.4799622714019307 2202 42-43 0.5474058048918877 2202 44-45 0.55204541428526 2202 46-47 0.5511677186818602 2202 48-49 0.5117564755277471 2202 50-51 0.5358605160967613 2202 52-53 0.52634878445879 2202 54-55 0.5369357260674619 2202 56-57 0.563989814032567 2202 58-59 0.6132886207495929 2202 60-61 0.598560127504129 2202 62-63 0.5931935948819174 2202 64-65 0.6352468839937231 2202 66-67 0.5819229796482404 2202 68-69 0.5498402275765883 2202 70-71 0.5640137056128154 2202 72-73 0.5668791454811952 2202 74-75 0.5807152526725119 2202 76-77 0.5217360413645906 2202 78-79 0.6051339053677438 2202 80-81 0.5721229698295645 2202 82-83 0.6151531528010707 2202 84-85 0.6181955122783549 2202 86-87 0.6121031518670961 2202 88-89 0.6419311193238428 2202 90-91 0.5973106381943936 2202 92-93 0.6636201043736563 2202 94-95 0.6634313468766706 2202 96-97 0.6680181928148201 2202 98-99 0.6916799423234181 2202 100-101 0.770954611546351 1102 1 0.10865686491582949 1102 2 0.1077935076686174 1102 3 0.09573117519496321 1102 4 0.13220491387623667 1102 5 0.1618288630648621 1102 6 0.15906863211454692 1102 7 0.1602136481933556 1102 8 0.20079833556918913 1102 9 0.1804990129437556 1102 10-11 0.18247448568818925 1102 12-13 0.1727275019855412 1102 14-15 0.2874559164899537 1102 16-17 0.3020836552889534 1102 18-19 0.29311535573602043 1102 20-21 0.3020661696683362 1102 22-23 0.30481219506933854 1102 24-25 0.29859882863117804 1102 26-27 0.3166866203265428 1102 28-29 0.34377766774463225 1102 30-31 0.35144814464357665 1102 32-33 0.3641382599577341 1102 34-35 0.3668805859042621 1102 36-37 0.3568925457089378 1102 38-39 0.3802147857442435 1102 40-41 0.38874748565744 1102 42-43 0.37097019275718424 1102 44-45 0.34232818145138566 1102 46-47 0.3888075391412471 1102 48-49 0.3707993108774019 1102 50-51 0.392516140288933 1102 52-53 0.3887989107527261 1102 54-55 0.399040623702831 1102 56-57 0.35671614301331545 1102 58-59 0.3637627135297521 1102 60-61 0.3855962507592636 1102 62-63 0.37363737310977996 1102 64-65 0.37927634435939694 1102 66-67 0.4294825404629492 1102 68-69 0.39535489756114117 1102 70-71 0.3566348337124765 1102 72-73 0.4266358787411306 1102 74-75 0.42603786842952474 1102 76-77 0.4175130275196466 1102 78-79 0.4494003446335242 1102 80-81 0.4604354942616382 1102 82-83 0.4927294959394075 1102 84-85 0.4318186325710016 1102 86-87 0.47203339004578737 1102 88-89 0.5003739687225561 1102 90-91 0.4153185200781806 1102 92-93 0.5168245552169921 1102 94-95 0.5121767832318369 1102 96-97 0.44956980868087015 1102 98-99 0.47810735463389165 1102 100-101 0.580054914964613 2305 1 0.12993599571323244 2305 2 0.11957638878509158 2305 3 0.1302986198424776 2305 4 0.13936756888210766 2305 5 0.15917130900604803 2305 6 0.201784416241928 2305 7 0.17249834022104693 2305 8 0.2172662346730192 2305 9 0.1983621116217833 2305 10-11 0.18713145618526994 2305 12-13 0.20537581341790911 2305 14-15 0.33493558858217654 2305 16-17 0.33805119151479346 2305 18-19 0.3635087402679815 2305 20-21 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-1.1368064179367252 1213 72-73 -1.1371966969678482 1213 74-75 -1.131750337702492 1213 76-77 -1.1834591302627757 1213 78-79 -1.25641468293599 1213 80-81 -1.2588268210139972 1213 82-83 -1.2437501287149288 1213 84-85 -1.1420197935010137 1213 86-87 -1.2012771626026861 1213 88-89 -1.3009971225468036 1213 90-91 -1.483395787729755 1213 92-93 -1.3297375235570428 1213 94-95 -1.2607802270241635 1213 96-97 -1.3706921497541273 1213 98-99 -1.430021633301564 1213 100-101 -1.3176597966102115 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9881.0 3 820.0 4 592.0 5 449.0 6 528.0 7 1755.0 8 2971.0 9 4743.0 10 7721.0 11 10445.0 12 10182.0 13 9809.0 14 9584.0 15 9712.0 16 10369.0 17 11613.0 18 13144.0 19 14606.0 20 16616.0 21 18624.0 22 21179.0 23 23763.0 24 26759.0 25 31053.0 26 35674.0 27 42698.0 28 51286.0 29 62956.0 30 80596.0 31 103745.0 32 139679.0 33 192737.0 34 273453.0 35 411295.0 36 857943.0 37 4155971.0 38 4377968.0 39 2095.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 22.726092369366967 14.899400528867861 21.28949922399805 41.085007877767126 2 25.79177016248239 25.201952701566853 29.772494580055763 19.233782555894987 3 23.435229165085413 26.800314976662055 30.30886841898852 19.45558743926401 4 25.05252520915654 30.018577098764244 22.36033593307643 22.56856175900279 5 28.038454128386768 33.230453485636815 21.72480356316807 17.00628882280835 6 19.277917507854607 37.778171630234006 22.072801805192647 20.87110905671874 7 18.507173771427738 16.31985340099246 42.92450493446688 22.24846789311292 8 22.355135139100316 21.834978903859216 28.572684806205217 27.237201150835254 9 24.54182895279653 22.89662005596183 26.933126114266642 25.628424876974996 10-11 24.25408983550847 31.21333778524914 22.0957360472637 22.436836331978686 12-13 22.852779511153138 25.351864132023206 27.83006072467605 23.96529563214761 14-15 23.17411711055467 26.85842723785765 27.79259271606299 22.174862935524686 16-17 24.01465950752679 26.80756203022674 26.876941487892005 22.300836974354468 18-19 23.78716248718816 27.047520803781776 26.789146068828547 22.37617064020152 20-21 23.823358233419217 27.309723674972663 26.653132583045785 22.213785508562335 22-23 23.890051042398174 27.155862202420845 26.788084064428574 22.16600269075241 24-25 23.448415748337368 27.51571677517755 26.859347318536425 22.176520157948655 26-27 23.884450232383887 27.64713120859051 26.397300425654795 22.07111813337081 28-29 23.964868290059325 26.9799748274956 26.83673696327303 22.218419919172046 30-31 23.501103483903215 27.25210209778527 26.929398988946595 22.317395429364918 32-33 23.706373891022043 27.3379837782045 26.76749765052128 22.18814468025218 34-35 24.01511073161482 26.916939479947473 26.849396426232445 22.218553362205267 36-37 23.562312007153892 27.14180222682713 27.039720257360695 22.256165508658285 38-39 24.014860355583846 27.218551603562137 26.764967275124867 22.00162076572915 40-41 24.08712939683865 26.760758318385026 26.84338967705586 22.30872260772046 42-43 23.75284918838926 26.908210531903915 27.05844900966852 22.280491270038308 44-45 24.154822333416153 27.011678321317284 26.750029505349048 22.083469839917516 46-47 24.06164711968614 26.921311168958766 26.80439518598508 22.212646525370015 48-49 23.87082332429519 26.857323726106152 26.98145436997391 22.290398579624746 50-51 24.15664304276077 27.086910934828683 26.496664990867636 22.259781031542907 52-53 24.270995617831744 26.872927264121778 26.720168135570628 22.135908982475854 54-55 23.84901539644735 26.995441814139227 26.93744632111664 22.218096468296785 56-57 24.222965043840077 27.092613070022427 26.804672604732293 21.8797492814052 58-59 24.47239373446604 27.01039711113527 26.710447964478455 21.806761189920234 60-61 23.938348621992194 27.11939516462053 26.677583773688244 22.264672439699027 62-63 24.600912955655566 27.174001177174866 26.400127088888926 21.82495877828064 64-65 24.59511066434833 27.07947547634572 26.564872672607404 21.760541186698543 66-67 24.415436274671375 27.234726664809745 26.63759430550329 21.71224275501559 68-69 24.87111480026541 27.25975969925142 26.363655627910838 21.50546987257234 70-71 24.94478709803595 27.25386888701365 26.266143752738913 21.535200262211486 72-73 24.83394326743582 27.29686912567178 26.23257667458422 21.63661093230818 74-75 25.173001781250676 27.429358313625567 26.0147515327175 21.382888372406253 76-77 25.449199590211354 27.33103081668971 26.06554063279855 21.15422896030039 78-79 25.35643260212137 27.355753016504657 26.12590050415507 21.161913877218904 80-81 25.83314348520233 27.50181006664677 25.563561403315727 21.10148504483518 82-83 26.077125802077873 27.46622517245962 25.559250018281137 20.897399007181374 84-85 26.02824705503458 27.469084026546696 25.637196886540302 20.86547203187842 86-87 26.40138236274018 27.60970457620133 25.320114216444527 20.66879884461396 88-89 26.730153296210407 27.497888684662996 25.256869529542342 20.515088489584254 90-91 26.833663917447797 27.471211554735692 25.249183579144646 20.44594094867186 92-93 27.375164787845623 27.6341344197917 24.717345971048957 20.273354821313717 94-95 27.60492174523959 27.54651604737187 24.82707630014597 20.02148590724257 96-97 27.58250968650686 27.723606026165715 24.84553410071957 19.848350186607856 98-99 28.219402643219116 27.72228972383838 24.508522611642935 19.549785021299567 100-101 28.493900609135526 27.63862187144806 24.348525859252405 19.518951660164007 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 8294.0 1 4587.5 2 695.5 3 457.0 4 353.0 5 295.0 6 281.0 7 291.0 8 282.0 9 265.5 10 306.0 11 346.0 12 351.5 13 378.0 14 429.5 15 501.5 16 578.5 17 660.5 18 753.5 19 874.5 20 1069.0 21 1304.5 22 1685.5 23 2360.0 24 3326.0 25 4619.5 26 6823.5 27 10124.0 28 14912.5 29 21377.5 30 29665.5 31 40151.0 32 52061.5 33 65806.0 34 82953.5 35 103644.5 36 132138.0 37 168850.5 38 212413.0 39 265829.5 40 332773.5 41 408629.5 42 486905.5 43 567029.5 44 639776.0 45 698126.0 46 735020.5 47 745855.0 48 733059.0 49 698945.5 50 644532.0 51 576790.5 52 500189.5 53 422898.5 54 348924.5 55 283831.0 56 231831.5 57 190042.0 58 155716.0 59 124767.5 60 97832.0 61 69526.0 62 43714.5 63 27725.0 64 17862.0 65 10745.5 66 7038.5 67 4434.5 68 2963.5 69 2356.0 70 1981.0 71 1475.5 72 871.0 73 691.0 74 679.5 75 537.5 76 366.0 77 183.5 78 86.5 79 67.5 80 53.0 81 37.0 82 32.5 83 31.5 84 28.5 85 24.0 86 18.5 87 10.0 88 8.0 89 10.5 90 9.5 91 6.5 92 4.0 93 3.0 94 2.5 95 3.0 96 1.5 97 0.0 98 0.0 99 0.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.13070087473430608 2 0.12944352671104714 3 0.12841232041859016 4 0.13638155501205154 5 0.13524180068880962 6 0.11840781024791104 7 0.11933951417881514 8 0.11711427954772377 9 0.12265022911775597 10-11 0.08667560258177873 12-13 0.09416541670593995 14-15 0.14682025730587045 16-17 0.14874698485230323 18-19 0.14831279272916345 20-21 0.18011284291453633 22-23 0.1523064556951262 24-25 0.14916308563697883 26-27 0.1374896494929812 28-29 0.14819067619453039 30-31 0.10476241821132022 32-33 0.11942997087113594 34-35 0.1325054857461058 36-37 0.13447744163869899 38-39 0.13722732508525093 40-41 0.16867459417057273 42-43 0.14982794232553662 44-45 0.13245573456532936 46-47 0.12439604327954719 48-49 0.14691071399819122 50-51 0.12225221967154451 52-53 0.12164615983299523 54-55 0.10777462606560244 56-57 0.10165523083010117 58-59 0.11284924650479863 60-61 0.11222057249316915 62-63 0.09747160881026475 64-65 0.11410207169344154 66-67 0.1053820465537176 68-69 0.11248289690089944 70-71 0.09492525292143457 72-73 0.10129340406081801 74-75 0.1333105503077608 76-77 0.16377184144678605 78-79 0.13082299126893915 80-81 0.12024860393663905 82-83 0.11046118982752985 84-85 0.10906815676578971 86-87 0.11280854099325428 88-89 0.12047926850205708 90-91 0.13759819752376612 92-93 0.1122250953277852 94-95 0.11241053154704282 96-97 0.1103978701429053 98-99 0.10172307334934175 100-101 0.09432371591750133 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.1055014E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.199053889640595 #Duplication Level Percentage of deduplicated Percentage of total 1 70.22518061289162 31.038865423189403 2 14.70889226921984 13.002382441283311 3 5.444570758205852 7.219346290437054 4 2.7791486653510877 4.913429665087018 5 1.6708964114909592 3.692602026774799 6 1.00160198410139 2.6561916042760965 7 0.6471195880175106 2.0021451480679575 8 0.5489550972053592 1.9410636739498044 9 0.4104101982029638 1.6325768220507786 >10 2.3090062918002707 18.646485561851353 >50 0.17347981965437437 5.211913149372721 >100 0.07589517725496227 6.209630608281423 >500 0.0038252826701945654 1.1121802878219404 >1k 0.0010178439339108701 0.7211872975563426 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 3.07552753890678E-4 0.0 0.0 2 5.608314923888835E-4 0.0 0.0 3 7.236535385663012E-4 0.0 0.0 4 0.001736768492559123 0.0 0.0 5 0.001854362192576147 0.0 0.0 6 0.0024151936849650304 0.0 0.0 7 0.0029850708465859925 0.0 0.0 8 0.00439619524679028 0.0 0.0 9 0.004884661385322534 0.0 0.0 10-11 0.005676157443129425 0.0 0.0 12-13 0.006426947989391963 0.0 9.045669232078765E-6 14-15 0.007381266093376273 0.0 9.045669232078765E-6 16-17 0.008747162147420166 9.045669232078765E-6 9.045669232078765E-6 18-19 0.010927168432351149 9.045669232078765E-6 9.045669232078765E-6 20-21 0.013283565267307666 9.045669232078765E-6 9.045669232078765E-6 22-23 0.015802784148441603 1.809133846415753E-5 9.045669232078765E-6 24-25 0.019154204598926784 2.7137007696236295E-5 9.045669232078765E-6 26-27 0.023939363622696455 3.618267692831506E-5 9.045669232078765E-6 28-29 0.03511981079354581 5.427401539247259E-5 1.3568503848118148E-5 30-31 0.05552231774649946 6.331968462455135E-5 1.809133846415753E-5 32-33 0.08454534747762418 6.331968462455135E-5 1.809133846415753E-5 34-35 0.12004507637891729 6.331968462455135E-5 1.809133846415753E-5 36-37 0.16876052802827748 6.331968462455135E-5 1.809133846415753E-5 38-39 0.23352751972996144 6.331968462455135E-5 1.809133846415753E-5 40-41 0.3172406656382344 6.331968462455135E-5 1.809133846415753E-5 42-43 0.4250831342230774 7.236535385663012E-5 1.809133846415753E-5 44-45 0.5531652877146966 7.236535385663012E-5 1.809133846415753E-5 46-47 0.7024052615401482 7.236535385663012E-5 1.809133846415753E-5 48-49 0.8813964414699067 7.68881884726695E-5 1.809133846415753E-5 50-51 1.0870316401227533 8.141102308870889E-5 1.809133846415753E-5 52-53 1.3271986810690606 8.141102308870889E-5 1.809133846415753E-5 54-55 1.6017437879318832 9.045669232078766E-5 1.809133846415753E-5 56-57 1.9108116914189344 9.045669232078766E-5 1.809133846415753E-5 58-59 2.255781856088106 9.045669232078766E-5 1.809133846415753E-5 60-61 2.647974936983345 9.045669232078766E-5 1.809133846415753E-5 62-63 3.0929133151708355 9.045669232078766E-5 1.809133846415753E-5 64-65 3.5949931858973674 9.950236155286642E-5 1.809133846415753E-5 66-67 4.152699399566568 9.950236155286642E-5 1.809133846415753E-5 68-69 4.773594135656454 1.1759370001702394E-4 1.809133846415753E-5 70-71 5.47426715153866 1.1759370001702394E-4 2.7137007696236295E-5 72-73 6.263614862902933 1.1759370001702394E-4 2.7137007696236295E-5 74-75 7.145436450826747 1.1759370001702394E-4 2.7137007696236295E-5 76-77 8.116502611394251 1.266393692491027E-4 2.7137007696236295E-5 78-79 9.185800216987513 1.266393692491027E-4 2.7137007696236295E-5 80-81 10.364514237612001 1.266393692491027E-4 2.7137007696236295E-5 82-83 11.652707992952339 1.3568503848118148E-4 2.7137007696236295E-5 84-85 13.044054037380686 1.3568503848118148E-4 2.7137007696236295E-5 86-87 14.517421687570907 1.3568503848118148E-4 2.7137007696236295E-5 88-89 16.05225918302772 1.3568503848118148E-4 2.7137007696236295E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGGCGC 880 0.0 15.385218 42-43 GGCGCCC 410 0.0 15.060929 44-45 TCGGGGG 1740 0.0 14.063166 38-39 GGGGGGC 1180 0.0 12.4824505 40-41 CAAAAAT 10005 0.0 11.727106 1 GCGCCCG 520 0.0 11.418235 44-45 GGGGGCG 1300 0.0 10.781988 40-41 CCCGCTA 1210 0.0 10.204153 7 CGGGGGT 840 0.0 10.181653 38-39 GGGCCCG 350 9.712858E-8 10.17854 44-45 CGGCAAC 3035 0.0 10.1733885 1 CGAAAAT 6470 0.0 10.131781 1 AGGGTAG 2260 0.0 9.773266 28-29 CGAAAAC 6555 0.0 9.493134 1 CGGGGGG 1610 0.0 9.443852 38-39 GGGGCCC 510 3.8744474E-10 9.313697 44-45 TAAACGT 3725 0.0 9.179704 8 CGGGCAT 785 5.135571E-7 9.076797 1 CGACAAT 5725 0.0 8.961057 1 CTCGGCT 4140 0.0 8.949634 1 >>END_MODULE