FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l03n02 tsrexp_lib#11.3420000000cb76.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l03n02 tsrexp_lib#11.3420000000cb76.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11055014
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGCGC8800.015.38521842-43
GGCGCCC4100.015.06092944-45
TCGGGGG17400.014.06316638-39
GGGGGGC11800.012.482450540-41
CAAAAAT100050.011.7271061
GCGCCCG5200.011.41823544-45
GGGGGCG13000.010.78198840-41
CCCGCTA12100.010.2041537
CGGGGGT8400.010.18165338-39
GGGCCCG3509.712858E-810.1785444-45
CGGCAAC30350.010.17338851
CGAAAAT64700.010.1317811
AGGGTAG22600.09.77326628-29
CGAAAAC65550.09.4931341
CGGGGGG16100.09.44385238-39
GGGGCCC5103.8744474E-109.31369744-45
TAAACGT37250.09.1797048
CGGGCAT7855.135571E-79.0767971
CGACAAT57250.08.9610571
CTCGGCT41400.08.9496341
Produced by FastQC (version 0.11.2)