FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l03n01 tsrexp_lib#8.3410000000cb29.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l03n01 tsrexp_lib#8.3410000000cb29.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10593195
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC116500.10997626306322125TruSeq Adapter, Index 5 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAC11100.017.537628
CGGGCCG2307.9317205E-414.4712351
GGCGCGC6150.014.29113838-39
CGGGGGT6155.456968E-1213.9165891
GCCCCGG5552.1282176E-1013.7076321
CGGGCAT17050.012.8282941
GGCCGTA14900.012.746287
CCCGGGG10900.012.2142531
CGCGACT10650.011.6080461
CGGGGTT20150.011.32665351
CGCGCTA3000.005835132711.0946141
CCCGGAT21450.010.8618611
CGCGAAT20200.010.8278411
CGCCAAT35300.010.7758281
CCCGGGT32250.010.7628811
CGGGAAT41800.010.57893851
GCGCGCA9300.010.47227738-39
CGCGCAC8900.010.40889340-41
GCGGGGT4601.1779295E-410.33659651
CTGGAAT65950.010.3099521
Produced by FastQC (version 0.11.2)