FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l03n01 tsrexp_lib#5.3410000000cabd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l03n01 tsrexp_lib#5.3410000000cabd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13499843
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC493790.36577462419377765TruSeq Adapter, Index 4 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCGTA19900.021.2340937
GCCGTAC20150.021.2062668
GCCCCGG7600.020.6432551
CGGGGGT7500.016.4812411
GCGGGGT5953.6379788E-1214.3824651
TGAAAAA192100.014.01020262-63
CGGGGCG3154.0364946E-513.583441
CGCGTAT11900.012.7844131
CGCCAAT47150.012.5031031
CGCCCCT10650.012.4993161
CCGTACA34700.012.4511279
GAAAAAA224400.012.35349762-63
GGCGCGC6400.012.24871138-39
CGCGGAT21000.012.2250971
CCGGGTT61000.012.1582931
TGGCCGT36350.011.8859456
CGCCCAT26850.011.8633761
CGGGGTG6905.293259E-1011.7132561
TTGAAAA237450.011.65472760-61
CCGGGAT42750.011.4545741
Produced by FastQC (version 0.11.2)