FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l03n01 tsrexp_lib#39.3410000000ce5d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l03n01 tsrexp_lib#39.3410000000ce5d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8835098
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGAT16650.025.9847931
CGCGCGT11850.020.4618071
CCGGGGT7800.019.5050721
CGCGCCT14900.017.8687381
CGGGGGT6600.017.2885861
CGGGCAT14850.016.3281081
GCGGCCC12750.014.8943698
CGCGGCT18150.014.6691021
GCGCCTG17950.014.5473992
CGGGCGA6650.014.2988291
GCGGATG30300.013.4754382
CCGGGAT25150.013.4218531
CGGGGGA12050.013.4147931
CGCGGTT16150.012.9530561
CCGGAAC39150.012.8725991
CTGGGAT35450.012.7408821
CCGGGAA43600.012.6492171
CGGGGTG4901.0975964E-712.613611
CCCGGGT9450.012.5776741
CCGCCCT8350.012.5264591
Produced by FastQC (version 0.11.2)