FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l03n01 tsrexp_lib#36.3410000000ce19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l03n01 tsrexp_lib#36.3410000000ce19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7528959
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC133860.17779350372342312TruSeq Adapter, Index 10 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGAT13600.024.8183961
CCGGGGT6300.019.6194611
CCGGGGA16750.014.7585191
CGCGGCT14850.014.4058991
CCCGTCT15150.013.493051
CGGGAAT23600.013.0934961
CCCGGGT8750.013.0393951
CGCGCCT9500.013.01081
CGCGAAT11050.012.9066421
CGGGTGT10000.012.8356541
CGGGCGT6351.3278623E-1012.7270991
CGCGGGT6752.7284841E-1112.677191
CGGGTAT13500.012.3250461
CGCGTAT7005.2750693E-1112.2244341
CGCGCGT7055.820766E-1112.1377351
CGGATGG24000.012.067383
CCGGAAC30700.011.9235781
CGGGGGA10400.011.8848661
GCGGATG26900.011.648932
CGGGCAT12300.011.5949911
Produced by FastQC (version 0.11.2)