FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l03n01 tsrexp_lib#33.3410000000cde1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l03n01 tsrexp_lib#33.3410000000cde1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6495659
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC260000.40026731698816087TruSeq Adapter, Index 9 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGAT12150.025.822311
CCGGGGT5150.019.3838251
CGCGCCT9700.019.1126231
CGCGGGG2253.5184137E-516.9018761
CGCGCGT6300.015.845511
CGCGGCT12000.015.0532341
CGGGCAT8300.014.890961
GCGGGTT14300.014.6266221
CGGGGGT5006.530172E-1014.2609581
CGCGTAT5501.8371793E-1013.8288071
GGGGGTC2751.9678504E-413.8130523
CGGGCGA4503.6092388E-813.7327741
CGCGGTT11900.013.5818631
CGGATGG19100.013.4243353
CGGGTGT8200.013.3334161
CGGGGTG3651.1251848E-513.0088682
TGAAAAA103450.012.85870762-63
GCGGACT5553.1141099E-912.847711
CGCGGTA9400.012.64269351
GGGAAAT30600.012.5831991
Produced by FastQC (version 0.11.2)