FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l03n01 tsrexp_lib#30.3410000000cd77.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l03n01 tsrexp_lib#30.3410000000cd77.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10816036
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC451870.41777782544362835TruSeq Adapter, Index 8 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGAT18400.031.5216961
CCGGGGT7000.019.0163671
CGCGCGT11450.017.0233861
CCCGGGT10450.015.4678581
CGCGCCT12550.015.1524831
CCGGGCT17650.014.814451
GCGGGGT7400.014.7762311
CGGATGG35350.014.7743463
CGGGGGT8500.014.5419281
GCGGGTT23350.014.2520951
CGCGTAT8600.013.8200351
TGAAAAA172450.013.73346562-63
CCCGACT21050.013.3250071
ATGGACG37500.013.2939196
CGGGCGT8600.013.2672341
CCGGACT19500.013.1651771
GCGGATG38950.013.1650152
CCGGGAT32350.013.0792641
CGCGGTA14650.012.9804551
CCCGGCT20700.012.6316441
Produced by FastQC (version 0.11.2)