FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l03n01 tsrexp_lib#25.3410000000cd27.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l03n01 tsrexp_lib#25.3410000000cd27.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11408286
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC307770.2697775984928849TruSeq Adapter, Index 3 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGAT24250.024.1120721
CCGGGGT9800.021.8286861
CGGGGGA15700.017.864611
CGCGCGT14750.016.1145861
CCCCGGT14000.015.61963751
GCGGGTT26700.015.4899191
CGCGGCT25900.015.2341961
CCCGGGA20850.015.048011
CCCCCCT6550.014.51542951
CCCGTGT17950.014.3011331
CGCGCCT17650.014.2748751
GCGGATG42000.014.2430322
CGGGCGA8150.013.9989291
CCCCCGT6551.8189894E-1213.7896581
CGGATGG37900.013.6542693
GCCGGGT16050.013.6246061
CCCGCCT23050.013.4055181
CCCGTAT16750.013.3390281
CCCCCAT28850.013.1821221
GCCCGCT8400.013.0163661
Produced by FastQC (version 0.11.2)