FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l03n01 tsrexp_lib#22.3410000000ccff.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l03n01 tsrexp_lib#22.3410000000ccff.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7639951
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC236440.3094784246652891TruSeq Adapter, Index 1 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGAT15950.023.548011
CGCGCGT9200.019.120611
CCGGGGT4750.018.0163461
CGGGGGT6550.017.4203851
CGCGCCT11000.016.8562031
CGGGGGA9550.015.9306831
CGCGGCT14700.015.84771251
GCGGGTT15300.015.84771251
CGCCCCC3755.7907528E-815.2138031
CGGGCGT7000.014.9421281
CCGGGTA13500.013.7346851
CGCGGGT6257.2759576E-1213.6924241
GCGGGGT5402.066372E-913.2064271
GCGGGAT11950.013.1290681
GCGGATG30100.013.0920542
GCGGCCC10950.013.0072758
CGGGTGT9900.012.966311
CTCGGGA28850.012.6891561
CGGATGG25150.012.6483253
CCCGGGT8350.012.5263361
Produced by FastQC (version 0.11.2)