FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l03n01 tsrexp_lib#2.3410000000ca44.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l03n01 tsrexp_lib#2.3410000000ca44.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17801708
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC1419270.7972661949066909TruSeq Adapter, Index 2 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAAAAA324300.022.9127162-63
CTGCTTG359500.021.14595256-57
GCCGTCT360800.020.78265448-49
TGCCGTC367100.020.22535748-49
ATGCCGT374400.019.94091646-47
TATGCCG374050.019.7690646-47
TTGAAAA383900.019.70880160-61
GAAAAAA384200.019.5753862-63
TGCTTGA392850.019.22952858-59
CCGTCTT403050.018.60469850-51
GTATGCC409150.018.25687444-45
CTTGAAA421150.017.87533860-61
GCTTGAA444500.017.28369158-59
CGTATGC432000.017.1647544-45
TTCTGCT463750.016.61993454-55
TCGTATG468650.016.00136442-43
CTTCTGC484300.015.85093354-55
CGTCTTC497100.015.39063950-51
TCTGCTT510300.015.06930856-57
CTCGTAT496450.014.88522942-43
Produced by FastQC (version 0.11.2)