FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l03n01 tsrexp_lib#19.3410000000cc85.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l03n01 tsrexp_lib#19.3410000000cc85.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8812976
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC227480.2581193912249392TruSeq Adapter, Index 12 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGGT7250.023.607731
CGCGCGT10000.022.8208071
CGCGGAT19200.022.5335671
CGGGGGA13700.020.4748731
CGGGCGT7200.017.8287561
GCGGGTT21450.017.7317851
GCGGCCC10250.017.6014278
CGCGCCT13100.016.694611
CGGGCGA7700.016.6710431
CGGGGGT6850.016.6575241
GCCGGGT13450.016.613661
CTCGGGA36550.015.7393851
CCCCCGT3951.0944677E-714.4435491
CCCGGGA14200.014.3969311
CGGGGCA8900.013.8890681
CCCGGCT20450.013.7166631
CGGATGG27400.013.5158853
CGCGGCT19000.013.51232051
GCGGGAA25300.013.3422051
CCCCCAT21950.013.21250251
Produced by FastQC (version 0.11.2)