FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l03n01 tsrexp_lib#16.3410000000cbf0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l03n01 tsrexp_lib#16.3410000000cbf0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22663879
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC830950.366640679647116TruSeq Adapter, Index 7 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGGT18650.019.8831521
CGCGGAT47250.019.016451
CGCGCGT32200.016.8313311
CGGGGGA30450.016.549621
CGCGCCT36250.016.2623441
CGGGGGT19250.016.0528491
GCGGGTT50200.015.6260691
GCGGGGT14250.015.0129881
CCCCCCT14000.013.9227581
CGGGTGT24900.013.5559041
CGCGGCT46500.013.3933071
CCGGGTT87550.013.3582151
GCCGGGT32250.013.2672921
GCGGCCC35600.013.0693238
GCGGGAT36750.013.0657241
TGAAAAA334400.012.93617862-63
CCCGGGT26150.012.9079171
CGGGCGT19650.012.8227991
CGCGTAT22250.012.820081
CCGGCCT42250.012.6026181
Produced by FastQC (version 0.11.2)