FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l03n01 tsrexp_lib#11.3410000000cb79.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l03n01 tsrexp_lib#11.3410000000cb79.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11055014
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC130900.11840781024791104TruSeq Adapter, Index 6 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGGGT5700.015.8459971
CGGGGCG2406.1130675E-515.8459961
CGCGTAT10000.015.2121551
CCCGGGG10250.014.8411271
CGGGGGT8050.014.763351
CGCGGAT19450.013.4426191
CGGGCCT8600.013.2664151
CGGGGCT13550.012.630021
CCGGGAT35400.012.6230811
CCCGCGT10250.012.5222021
CCGGGTT45650.012.0797521
CGGGCGA11050.012.0458261
CGGGCAT19400.012.0070191
GGCGCGC6150.011.974503538-39
CGCGGGT14250.011.6759971
CGGGGAC41700.011.6133242
CGCCCTA8701.8189894E-1211.4746871
CGGGGTT23800.011.3851481
CCCGGGA20050.011.3806661
CCCGAGT19700.011.341551
Produced by FastQC (version 0.11.2)